干旱胁迫下春大麦(Hordeum vulgare L.)根系结构特征的遗传控制。

IF 3.9 2区 生物学 Q1 GENETICS & HEREDITY
Plant Genome Pub Date : 2024-06-01 Epub Date: 2024-05-19 DOI:10.1002/tpg2.20463
Md Nurealam Siddiqui, Melisa Jahiu, Mohammad Kamruzzaman, Miguel Sanchez-Garcia, Annaliese S Mason, Jens Léon, Agim Ballvora
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引用次数: 0

摘要

根系结构特征在植物适应干旱胁迫的过程中起着关键作用,因此被认为是育种计划中很有前景的目标。在这里,我们对 200 个春大麦近交系在连续两个田间季节中对水分充足和干旱胁迫条件下的 8 个根系结构特征进行了表型分析。与对照处理相比,两个季节的根系结构特征在干旱条件下都发育较差。利用全基因组关联研究(GWAS)和连锁不平衡图谱分析了根系结构特征的遗传变异。全基因组关联研究发现,在对照、干旱和干旱相关指数下,共有 186 个显著的单核苷酸多态性与 8 个根系性状相关。其中,在 3 号和 5 号染色体上发现了几个根系性状位点,这些位点与之前研究中发现的 QTL 位于同一位置。有趣的是,有 13 个位点同时与干旱和干旱相关指数下的多个根系性状相关。这些基因座上的候选基因包括多种干旱响应成分,如转录因子、结合蛋白、蛋白激酶、养分和离子转运体以及胁迫信号转导因子。例如,两个候选基因 HORVU7Hr3G0713160 和 HORVU6H r3G0626550 与 AtACX3 和 AtVAMPs 同源,这两个基因据报道分别具有根长介导的抗旱功能和作为非生物胁迫耐受的关键蛋白的功能。有趣的是,这些基因座中的一个高置信度候选基因 "根缺损新促进基因1(NEW ENHANCER OF ROOT DWARFISM1,NERD1)"与根的发育有关。该基因座非编码区的等位基因变异与干旱条件下根长度的增加显著相关。总之,这些结果提供了有希望的多性状影响位点和根表型对干旱胁迫响应的候选基因,可为大麦耐旱性的遗传改良提供宝贵的资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.).

Root architectural traits play pivotal roles in plant adaptation to drought stress, and hence they are considered promising targets in breeding programs. Here, we phenotyped eight root architecture traits in response to well-watered and drought stress conditions in 200 spring barley (Hordeum vulgare L.) inbred lines over two consecutive field seasons. Root architecture traits were less developed under drought in both seasons when compared with control treatments. Genetic variation in root architectural traits was dissected employing a genome-wide association study (GWAS) coupled with linkage disequilibrium mapping. GWAS uncovered a total of 186 significant single nucleotide polymorphism-trait associations for eight root traits under control, drought, and drought-related indices. Of these, a few loci for root traits were detected on chromosomes 3 and 5, which co-located with QTL identified in previous studies. Interestingly, 13 loci showed simultaneou associations with multiple root traits under drought and drought-related indices. These loci harbored candidate genes, which included a wide range of drought-responsive components such as transcription factors, binding proteins, protein kinases, nutrient and ion transporters, and stress signaling factors. For instance, two candidate genes, HORVU7Hr3G0713160 and HORVU6H r3G0626550, are orthologous to AtACX3 and AtVAMPs, which have reported functions in root length-mediated drought tolerance and as a key protein in abiotic stress tolerance, respectively. Interestingly, one of these loci underlying a high-confidence candidate gene NEW ENHANCER OF ROOT DWARFISM1 (NERD1) showed involvement with root development. An allelic variation of this locus in non-coding region was significantly associated with increased root length under drought. Collectively, these results offer promising multi-trait affecting loci and candidate genes underlying root phenotypic responses to drought stress, which may provide valuable resources for genetic improvement of drought tolerance in barley.

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来源期刊
Plant Genome
Plant Genome PLANT SCIENCES-GENETICS & HEREDITY
CiteScore
6.00
自引率
4.80%
发文量
93
审稿时长
>12 weeks
期刊介绍: The Plant Genome publishes original research investigating all aspects of plant genomics. Technical breakthroughs reporting improvements in the efficiency and speed of acquiring and interpreting plant genomics data are welcome. The editorial board gives preference to novel reports that use innovative genomic applications that advance our understanding of plant biology that may have applications to crop improvement. The journal also publishes invited review articles and perspectives that offer insight and commentary on recent advances in genomics and their potential for agronomic improvement.
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