将斑马鱼作为研究高血糖状态下伤口愈合的模型

Pub Date : 2024-05-01 DOI:10.46542/pe.2024.243.111115
Lia Nurkhasanah, Farida Hayati, R. Istikharah
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引用次数: 0

摘要

背景:对于糖尿病伤口治疗的临床前研究,斑马鱼有许多在伤口愈合和再生中发挥重要作用的同源信号通路:本研究比较了高血糖斑马鱼(Danio rerio)和正常斑马鱼的四个重要伤口愈合基因的表达和尾鳍再生。在斑马鱼尾鳍截肢后的第 5 天用体视显微镜观察其再生情况,然后取样分析基因表达。使用 Zeiss Zen 3.3 蓝色记录仪分析尾鳍再生情况;使用血糖仪测量血糖水平;使用 qRT-PCR 方法分析声刺猬(shh)、胰岛素样生长因子 2a(igf2a)、骨形态发生蛋白 2b(bmp2b)、胶原 1a2(col1a2)的相对基因表达情况:结果:高血糖组的血糖水平为 203.2 mg/dL,正常组为 59.5 mg/dL。作者发现,在高血糖组中,igf2a、shh、bmp2b和col1a2的表达均下调,尾鳍再生能力下降:结论:这种新型的成年斑马鱼高血糖模型会严重影响基因表达和尾鳍再生。
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Zebrafish as a model for the study of wound healing in hyperglycemia
Background: For preclinical studies of diabetic wound therapy, zebrafish have many orthologous signalling pathways that play essential roles in wound healing and regeneration. Objectives: This study compares the expression of four essential wound-healing genes and caudal fin regeneration in hyperglycemic zebrafish (Danio rerio) to normal zebrafish. Methods: Hyperglycemia was induced by using the intraperitoneal injection method with 350 mg/BW streptozotocin on days one, three, and five. The regeneration of the zebrafish's caudal fin was observed on day 5 after amputation using a stereomicroscope, followed by sampling of the blastema to analyse gene expression. Caudal fin regeneration was analysed using Zeiss Zen 3.3 blue documentation; blood glucose levels were measured using a glucometer; and relative gene expression analysis of sonic hedgehog (shh), insulin-like growth factor 2a (igf2a), bone morphogenetic protein 2b (bmp2b), collagen 1a2 (col1a2) was performed by qRT-PCR method. Results: The glucose level in the hyperglycemia group was 203.2 mg/dL, and that in the normal group was 59.5 mg/dL. The authors found that igf2a, shh, bmp2b, and col1a2 expression were all downregulated in the hyperglycemia group and decreased in caudal fin regeneration. Conclusion: This novel adult zebrafish model of hyperglycemia significantly impairs gene expression and caudal fin regeneration.
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