TRIS和HEPES对兔肌轻肌浆网功能的影响。

Membrane biochemistry Pub Date : 1987-01-01 DOI:10.3109/09687688709039987

B S Selinsky, A D Messana, W Scherer, P L Yeagle

{"title":"TRIS和HEPES对兔肌轻肌浆网功能的影响。","authors":"B S Selinsky, A D Messana, W Scherer, P L Yeagle","doi":"10.3109/09687688709039987","DOIUrl":null,"url":null,"abstract":"ATP hydrolysis activity and calcium transport activity were determined on light sarcoplasmic reticulum from rabbit skeletal muscle. The effects of two buffers, TRIS and HEPES, were compared. Titration of TRIS into sarcoplasmic reticulum preparations in HEPES provided evidence for TRIS inhibition of ATPase activity and TRIS stimulation of calcium transport activity.","PeriodicalId":18448,"journal":{"name":"Membrane biochemistry","volume":"7 2","pages":"107-13"},"PeriodicalIF":0.0000,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688709039987","citationCount":"1","resultStr":"{\"title\":\"Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum.\",\"authors\":\"B S Selinsky, A D Messana, W Scherer, P L Yeagle\",\"doi\":\"10.3109/09687688709039987\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ATP hydrolysis activity and calcium transport activity were determined on light sarcoplasmic reticulum from rabbit skeletal muscle. The effects of two buffers, TRIS and HEPES, were compared. Titration of TRIS into sarcoplasmic reticulum preparations in HEPES provided evidence for TRIS inhibition of ATPase activity and TRIS stimulation of calcium transport activity.\",\"PeriodicalId\":18448,\"journal\":{\"name\":\"Membrane biochemistry\",\"volume\":\"7 2\",\"pages\":\"107-13\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/09687688709039987\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Membrane biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/09687688709039987\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Membrane biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/09687688709039987","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

摘要

测定了兔骨骼肌轻肌浆网ATP水解活性和钙转运活性。比较了TRIS和HEPES两种缓冲液的效果。将TRIS滴定到HEPES肌浆网制剂中提供了TRIS抑制atp酶活性和刺激钙转运活性的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum.

ATP hydrolysis activity and calcium transport activity were determined on light sarcoplasmic reticulum from rabbit skeletal muscle. The effects of two buffers, TRIS and HEPES, were compared. Titration of TRIS into sarcoplasmic reticulum preparations in HEPES provided evidence for TRIS inhibition of ATPase activity and TRIS stimulation of calcium transport activity.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Membrane biochemistry

自引率

0.00%

发文量