子宫肌瘤妇女血浆中褪黑激素的水平以及血管生成、抗氧化系统和脂质过氧化的一些指标

Q4 Medicine
B. Sokolov, A. Berbets, Shashi Kant, O. Yuzko
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The reduced glutathione level in the blood plasma of women with uterine leiomyoma was significantly lower (0.77 ± 0.13 µmol/L) compared to healthy women (1.02 ± 0.14 µmol/L) in the control group, while the concentrations of glutathione-S-transferase and glutathione peroxidase enzymes were higher (161.3 ± 22.3 ng/mL and 235.7 ± 35.9 ng/mL, respectively), whereas in the control group these indicators were 118.9 ± 18.0 ng/mL and 105.3 ± 41.2 ng/mL, respectively. The MDA content in women of the study group was higher, measuring 5.2 ± 0.8 nmol/L in plasma and 10.8 ± 1.1 nmol/L in erythrocytes compared to 2.3 ± 0.8 nmol/L and 5.3 ± 0.8 nmol/L in the control group, respectively. Conclusions. 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引用次数: 0

摘要

子宫肌瘤是最常见的妇科疾病之一,通常会导致丧失生育能力。众所周知,在这种病理情况下,子宫组织的损伤伴随着氧化应激,而氧化应激的补偿机制在子宫肌瘤的再生过程中起着决定性作用,尤其是在进行器官保留手术时。本研究的目的是测定被诊断患有子宫肌瘤的育龄妇女体内褪黑激素、生殖激素的水平以及血管生成、抗氧化系统和脂质过氧化的状态。材料和方法研究对象为 60 名患有子宫肌瘤的育龄妇女(研究组)。对照组由 20 名同年龄组的健康妇女组成。早上 8 点空腹测定一次妇女血液中的褪黑激素水平;还测定了月经周期卵泡期的雌二醇和孕酮浓度。通过检测血浆中血管内皮生长因子(VEGF)的水平和一氧化氮最终代谢物 NO 的含量,研究了血管生成系统的状态。抗氧化系统的活性通过血浆中还原型谷胱甘肽、谷胱甘肽过氧化物酶和谷胱甘肽转移酶的浓度进行评估。血浆和红细胞中丙二醛(MDA)的含量是脂质过氧化的指标。结果发现研究发现,与对照组(153.5 ± 8.5 ng/mL),而雌二醇浓度(107.4 ± 25.3 pg/mL)几乎是对照组(36.2 ± 3.2 pg/mL)的三倍,孕酮浓度是对照组(2.1 ± 0.4 ng/mL,对照组为 1.1 ± 0.5 ng/mL)的 1.9 倍。子宫肌瘤妇女血浆中血管内皮生长因子的水平(90.4 ± 23.6 pg/mL)也高于对照组(35.1 ± 8.3 pg/mL),一氧化氮最终代谢物的浓度也较高,达到 25.3 ± 5.9 pg/mL,而对照组为 9.9 ± 3.9 pg/mL。与健康妇女(1.02 ± 0.14 µmol/L)相比,子宫纵膈肌瘤妇女血浆中的还原型谷胱甘肽水平明显较低(0.77 ± 0.13 µmol/L)。14 µmol/L),而谷胱甘肽-S-转移酶和谷胱甘肽过氧化物酶的浓度较高(分别为 161.3 ± 22.3 ng/mL 和 235.7 ± 35.9 ng/mL),而对照组中这些指标分别为 118.9 ± 18.0 ng/mL 和 105.3 ± 41.2 ng/mL。研究组妇女的 MDA 含量较高,血浆中为 5.2 ± 0.8 nmol/L,红细胞中为 10.8 ± 1.1 nmol/L,而对照组分别为 2.3 ± 0.8 nmol/L 和 5.3 ± 0.8 nmol/L。结论与健康妇女相比,子宫纵膈肌瘤妇女血浆中褪黑激素和还原型谷胱甘肽的水平明显较低,而雌二醇、孕酮、谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶、血管内皮生长因子、一氧化氮的最终代谢产物以及血浆和红细胞中平均 MDA 含量的浓度则明显较高。在研究组中,血浆中孕酮水平与红细胞中丙二醛含量之间的关系用线性回归方程来描述,正如我们所认为的,这表明孕酮对子宫肌层氧化应激机制有激活作用。我们认为,子宫肌瘤中的氧化应激是由于抗氧化系统(褪黑激素是其中不可或缺的一部分)的活性不足导致的,在雌激素诱导子宫肌瘤增殖的背景下,褪黑激素导致血管生成增强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Levels of melatonin and some indicators of angiogenesis, antioxidant system and lipid peroxidation in blood plasma in women with uterine leiomyoma
Uterine leiomyoma is one of the most common gynaecological diseases, which often leads to loss of fertility. It is known that in this pathology, damage to the tissues of the uterus is accompanied by oxidative stress, and the mechanisms of its compensation play a decisive role in the process of myometrial regeneration, especially when performing organ-preserving operations. The aim of the study was to determine the levels of melatonin, reproductive hormones and state of angiogenesis, antioxidant system and lipid peroxidation in women of reproductive age who were diagnosed with uterine leiomyoma. Material and methods. 60 women of reproductive age with uterine leiomyoma (study group) were examined. The control group consisted of 20 healthy women of the same age group. Melatonin levels in women's blood were determined once, on an empty stomach, at 8 a.m.; the concentrations of estradiol and progesterone in the follicular phase of the menstrual cycle were also measured. The state of the angiogenesis system was studied by examining the levels of vascular endothelial growth factor (VEGF) and the content of the final metabolites of nitric oxide NO in blood plasma. The activity of the antioxidant system was assessed by blood plasma concentrations of reduced glutathione, glutathione peroxidase, and glutathione transferase. Indicators of lipid peroxidation were investigated by the content of malondialdehyde (MDA) in blood plasma and erythrocytes. Results. It was found that in the patients of the study group with leiomyoma, there was a significantly lower level of melatonin in blood plasma (111.1 ± 18.5 ng/mL) compared to the control group (153.5 ± 8.5 ng/mL), while the concentration of estradiol was almost three times higher (107.4 ± 25.3 pg/mL) compared to the control group (36.2 ± 3.2 pg/mL), and the concentration of progesterone was 1.9 times higher (2.1 ± 0.4 ng/mL compared to 1.1 ± 0.5 ng/mL in the control group). The level of VEGF in blood plasma in women with uterine leiomyoma was also higher (90.4 ± 23.6 pg/mL) compared to the control group (35.1 ± 8.3 pg/mL), as well as the concentration of final metabolites of nitric oxide, which reached 25.3 ± 5.9 pg/mL compared to 9.9 ± 3.9 pg/mL in the control group. The reduced glutathione level in the blood plasma of women with uterine leiomyoma was significantly lower (0.77 ± 0.13 µmol/L) compared to healthy women (1.02 ± 0.14 µmol/L) in the control group, while the concentrations of glutathione-S-transferase and glutathione peroxidase enzymes were higher (161.3 ± 22.3 ng/mL and 235.7 ± 35.9 ng/mL, respectively), whereas in the control group these indicators were 118.9 ± 18.0 ng/mL and 105.3 ± 41.2 ng/mL, respectively. The MDA content in women of the study group was higher, measuring 5.2 ± 0.8 nmol/L in plasma and 10.8 ± 1.1 nmol/L in erythrocytes compared to 2.3 ± 0.8 nmol/L and 5.3 ± 0.8 nmol/L in the control group, respectively. Conclusions. The levels of melatonin and reduced glutathione in the blood plasma of women with uterine leiomyoma were significantly lower, while the concentrations of estradiol, progesterone, glutathione-S-transferase, glutathione peroxidase, VEGF, final metabolites of nitric oxide, as well as the average MDA content in plasma and erythrocytes, were significantly higher compared to healthy women. In the study group, the relationship between the level of progesterone in blood plasma and the content of malondialdehyde in erythrocytes was described using a linear regression equation, which, as we suggest, indicates an activating effect of progesterone on oxidative stress mechanisms in the myometrium. In our opinion, oxidative stress in uterine leiomyoma occurs due to inadequate activity of the antioxidant system, an integral part of which is melatonin, leading to enhanced angiogenesis against the background of estrogen-induced myometrial proliferation.
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来源期刊
Cell and Organ Transplantology
Cell and Organ Transplantology Medicine-Transplantation
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