{"title":"实验室获得的万古霉素中间型金黄色葡萄球菌菌株的特征","authors":"Sutanya Jongutsa, Suwanna Trakulsomboon, S. Pannanusorn, Ruthada Chanklan, Sudathip Chantorn, Worawat Surarit, Thrissawan Traijitt, Sugunya Utaida","doi":"10.59796/jcst.v14n2.2024.21","DOIUrl":null,"url":null,"abstract":"Four clinical vancomycin susceptible Staphylococcus aureus (VSSA) isolates were subjected to selection for the vancomycin-intermediate S. aureus (VISA) phenotype using increasing concentrations of vancomycin. The vancomycin MIC achieved for VISA strains was 7 µg/mL. Population analysis profiles of the bacteria revealed that almost 100% of population growing at 4 µg/mL of vancomycin while some subpopulations were found to be resistant to concentrations ranging from 6 to 9 µg/mL of vancomycin. The results correlated with the AUC ratios for VISA, which ranged from 3.02-3.3 in this study. In the absence of vancomycin in the assay buffer, all the laboratory-derived VISA strains exhibited reduced whole cell autolysis compared to that of their VSSA counterparts. Examination of cell wall morphologies revealed that almost all the laboratory-derived VISA strains had thicker and rougher cell wall surfaces compared to their parental strains. Vancomycin had no remarkable effects on the thickness and roughness of the cell wall of all the laboratory-derived VISA derivatives.","PeriodicalId":36369,"journal":{"name":"Journal of Current Science and Technology","volume":"18 3","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characteristics of Laboratory-Derived Vancomycin-Intermediate Staphylococcus aureus Strains\",\"authors\":\"Sutanya Jongutsa, Suwanna Trakulsomboon, S. Pannanusorn, Ruthada Chanklan, Sudathip Chantorn, Worawat Surarit, Thrissawan Traijitt, Sugunya Utaida\",\"doi\":\"10.59796/jcst.v14n2.2024.21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Four clinical vancomycin susceptible Staphylococcus aureus (VSSA) isolates were subjected to selection for the vancomycin-intermediate S. aureus (VISA) phenotype using increasing concentrations of vancomycin. The vancomycin MIC achieved for VISA strains was 7 µg/mL. Population analysis profiles of the bacteria revealed that almost 100% of population growing at 4 µg/mL of vancomycin while some subpopulations were found to be resistant to concentrations ranging from 6 to 9 µg/mL of vancomycin. The results correlated with the AUC ratios for VISA, which ranged from 3.02-3.3 in this study. In the absence of vancomycin in the assay buffer, all the laboratory-derived VISA strains exhibited reduced whole cell autolysis compared to that of their VSSA counterparts. Examination of cell wall morphologies revealed that almost all the laboratory-derived VISA strains had thicker and rougher cell wall surfaces compared to their parental strains. Vancomycin had no remarkable effects on the thickness and roughness of the cell wall of all the laboratory-derived VISA derivatives.\",\"PeriodicalId\":36369,\"journal\":{\"name\":\"Journal of Current Science and Technology\",\"volume\":\"18 3\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Current Science and Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.59796/jcst.v14n2.2024.21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Multidisciplinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Current Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.59796/jcst.v14n2.2024.21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Multidisciplinary","Score":null,"Total":0}
引用次数: 0
摘要
使用浓度不断增加的万古霉素,对四种临床万古霉素敏感金黄色葡萄球菌(VSSA)分离株进行万古霉素中间型金黄色葡萄球菌(VISA)表型选择。VISA 菌株的万古霉素 MIC 值为 7 µg/mL。细菌的种群分析图显示,几乎 100%的种群在万古霉素浓度为 4 µg/mL 时都能生长,而一些亚种群则对浓度为 6 至 9 µg/mL 的万古霉素具有耐药性。这些结果与 VISA 的 AUC 比率相关,在本研究中,VISA 的 AUC 比率为 3.02-3.3。在检测缓冲液中没有万古霉素的情况下,与 VSSA 菌株相比,所有实验室衍生的 VISA 菌株都表现出较低的全细胞自溶能力。细胞壁形态检查显示,与亲本菌株相比,几乎所有实验室衍生的 VISA 菌株的细胞壁表面都更厚、更粗糙。万古霉素对所有实验室衍生的 VISA 菌株的细胞壁厚度和粗糙度没有明显影响。
Characteristics of Laboratory-Derived Vancomycin-Intermediate Staphylococcus aureus Strains
Four clinical vancomycin susceptible Staphylococcus aureus (VSSA) isolates were subjected to selection for the vancomycin-intermediate S. aureus (VISA) phenotype using increasing concentrations of vancomycin. The vancomycin MIC achieved for VISA strains was 7 µg/mL. Population analysis profiles of the bacteria revealed that almost 100% of population growing at 4 µg/mL of vancomycin while some subpopulations were found to be resistant to concentrations ranging from 6 to 9 µg/mL of vancomycin. The results correlated with the AUC ratios for VISA, which ranged from 3.02-3.3 in this study. In the absence of vancomycin in the assay buffer, all the laboratory-derived VISA strains exhibited reduced whole cell autolysis compared to that of their VSSA counterparts. Examination of cell wall morphologies revealed that almost all the laboratory-derived VISA strains had thicker and rougher cell wall surfaces compared to their parental strains. Vancomycin had no remarkable effects on the thickness and roughness of the cell wall of all the laboratory-derived VISA derivatives.