Gil Martínez-Bautista, Pamela Padilla, Warren W. Burggren
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We compared morphological variability (yolk–chorion ratio, body mass, embryo mass, total length, condition factor, and specific growth rate) in wild-type AB and NHGRI-1 zebrafish lines as a function of their responses to altered temperature and oxygen availability during the first 7 days post-fertilization. Overall, both lines showed similar developmental trajectories for yolk–chorion ratio, embryo mass, and total length. Additionally, condition factor and specific growth rate showed similar responses within each line, regardless of temperature and hypoxia. Importantly, the coefficient of variation for each variable was significantly lower in NHGRI-1 than AB larvae for 151 of 187 assessed morphological endpoints. Thus, the low-heterozygosity NHGRI-1 zebrafish line can be useful for decreasing inter-individual variation in morphological responses to environmental stressors, thereby aiding in the interpretation of results and enhancing experimental reproducibility.","PeriodicalId":505604,"journal":{"name":"Fishes","volume":"20 29","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic Basis for Morphological Variation in the Zebrafish Danio rerio: Insights from a Low-Heterozygosity Line\",\"authors\":\"Gil Martínez-Bautista, Pamela Padilla, Warren W. Burggren\",\"doi\":\"10.3390/fishes9050164\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Data variability complicates reproducibility and the interpretation of experimental results. Different animal models have been employed to decrease variability to enhance experimental power. However, variation frequently persists among and within strains/lines. In zebrafish (Danio rerio), inbred lines (e.g., NHGRI-1) derived from wild-type lines have been produced to greatly decrease genetic variation, with the goal of providing better understanding of genetic backgrounds that may influence the experimental outcome of studies employing such lines. We hypothesized that variations in morphological phenotypes shaped by environmental stressors early in development are correlated with the intrinsic degree of genetic variability of zebrafish lines. We compared morphological variability (yolk–chorion ratio, body mass, embryo mass, total length, condition factor, and specific growth rate) in wild-type AB and NHGRI-1 zebrafish lines as a function of their responses to altered temperature and oxygen availability during the first 7 days post-fertilization. Overall, both lines showed similar developmental trajectories for yolk–chorion ratio, embryo mass, and total length. Additionally, condition factor and specific growth rate showed similar responses within each line, regardless of temperature and hypoxia. Importantly, the coefficient of variation for each variable was significantly lower in NHGRI-1 than AB larvae for 151 of 187 assessed morphological endpoints. 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引用次数: 0
摘要
数据的可变性使实验结果的可重复性和解释变得复杂。人们采用不同的动物模型来降低变异性,以提高实验能力。然而,不同品系/品系之间和品系内部经常存在变异。在斑马鱼(Danio rerio)中,从野生型品系中培育出的近交系(如 NHGRI-1)大大减少了遗传变异,目的是让人们更好地了解可能会影响使用这些品系进行研究的实验结果的遗传背景。我们假设,斑马鱼在发育早期受环境胁迫因素影响而产生的形态表型变化与斑马鱼品系的内在遗传变异程度相关。我们比较了野生型 AB 和 NHGRI-1 斑马鱼品系的形态变异性(卵黄-绒毛比率、体质量、胚胎质量、总长度、条件因子和特定生长率)与它们在受精后 7 天内对温度和氧气供应变化的反应的函数关系。总体而言,两个品系在卵黄-绒毛比率、胚胎质量和总长度方面表现出相似的发育轨迹。此外,无论温度和缺氧情况如何,每个品系的条件因子和特定生长率都表现出相似的反应。重要的是,在187个评估的形态学终点中,NHGRI-1幼虫在151个终点的每个变量的变异系数都明显低于AB幼虫。因此,低杂合度的NHGRI-1斑马鱼品系可以减少个体间对环境胁迫的形态反应差异,从而有助于解释结果并提高实验的可重复性。
Genetic Basis for Morphological Variation in the Zebrafish Danio rerio: Insights from a Low-Heterozygosity Line
Data variability complicates reproducibility and the interpretation of experimental results. Different animal models have been employed to decrease variability to enhance experimental power. However, variation frequently persists among and within strains/lines. In zebrafish (Danio rerio), inbred lines (e.g., NHGRI-1) derived from wild-type lines have been produced to greatly decrease genetic variation, with the goal of providing better understanding of genetic backgrounds that may influence the experimental outcome of studies employing such lines. We hypothesized that variations in morphological phenotypes shaped by environmental stressors early in development are correlated with the intrinsic degree of genetic variability of zebrafish lines. We compared morphological variability (yolk–chorion ratio, body mass, embryo mass, total length, condition factor, and specific growth rate) in wild-type AB and NHGRI-1 zebrafish lines as a function of their responses to altered temperature and oxygen availability during the first 7 days post-fertilization. Overall, both lines showed similar developmental trajectories for yolk–chorion ratio, embryo mass, and total length. Additionally, condition factor and specific growth rate showed similar responses within each line, regardless of temperature and hypoxia. Importantly, the coefficient of variation for each variable was significantly lower in NHGRI-1 than AB larvae for 151 of 187 assessed morphological endpoints. Thus, the low-heterozygosity NHGRI-1 zebrafish line can be useful for decreasing inter-individual variation in morphological responses to environmental stressors, thereby aiding in the interpretation of results and enhancing experimental reproducibility.