用纳米银颗粒处理的莪术小根的抗氧化活性增强

Sonam Patel, Afreen Anjum, Veenu Joshi, A. Quraishi
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引用次数: 0

摘要

莪术(Curcuma caesia Roxb.在本研究中,研究了添加了 1 毫克/升吲哚-3-丁酸(IBA)和不同蔗糖浓度(1.5%、3%、6%、9% 或 12%)的半强度液体 Murashige and Skoog(MS)培养基对莪术根状茎诱导的影响。蔗糖浓度在 6% 及以上时,铯尾草的芽长、根长和微菌体干重都明显下降。与对照组(1.5% 蔗糖)相比,蔗糖浓度为 6% 时,当前含水量明显降低。蔗糖浓度为 6%时,是体外诱导茜草微根状茎的最佳浓度。因此,在进一步的实验中,我们使用了 6% 的蔗糖。我们还研究了银纳米粒子(AgNP)对草履虫培养物微茎诱导和抗氧化活性的影响。银纳米粒子的绿色合成采用了野外生长的铯根茎提取物。通过扫描电子显微镜和 X 射线衍射对合成的 AgNP 进行了进一步表征。将 0、0.025、0.05、0.075 或 0.1 mg/l 的 AgNP 添加到含 6% 蔗糖和 1 mg/l IBA 的 ½ 强度液体 MS 培养基中。在添加 0.05 毫克/升 AgNP 的 MS 培养基中,茜草培养物的形态发生了显著变化(根数、根长和微根茎鲜重)。在总酚和总萜类化合物含量评估以及抗氧化活性分析中,使用了未经处理的培养物(6% 蔗糖 + 1 mg/l IBA,不含 AgNP)和 AgNP 处理的培养物(6% 蔗糖 + 1 mg/l IBA,含 0.025 和 0.05 mg/l AgNP)的提取物。与田间生长的母株相比,0.025 和 0.05 毫克/升 AgNP 提高了培养物中的酚类和萜类化合物含量。与未处理的培养物和田间生长的母株相比,经 AgNP 处理的培养物的抗氧化活性也有所提高。气相色谱-质谱(GC-MS)分析表明,用 0.05 毫克/升 AgNP 处理的提取物增加了单萜(樟脑)和倍半萜(β-榄香烯酮和莪术酮)的生成。这些增加的萜烯可能是 C. caesia 培养物抗氧化活性增强的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced antioxidant activity in Curcuma caesia Roxb. microrhizomes treated with silver nanoparticles
Curcuma caesia Roxb. is a highly valuable, endangered herb of therapeutic importance that resides in their rhizomes. In the present investigation, the effect of ½ strength liquid Murashige and Skoog (MS) medium supplemented with 1 mg/l Indole-3-butyric acid (IBA) and different sucrose concentrations (1.5%, 3%, 6%, 9%, or 12%) was studied on microrhizomes induction of C. caesia. The shoot length, root length and microrhizomes dry weight of C. caesia decreased significantly at 6% sucrose and above. When compared to the control (1.5% sucrose), the current water content significantly decreased at 6% sucrose. The optimum concentration for in vitro microrhizomes induction in C. caesia was 6% sucrose. Therefore for further experiments, the 6% sucrose was used. We also studied the effect of silver nanoparticles (AgNP) on microrhizome induction and antioxidant activity in C. caesia cultures. Field-grown C. caesia rhizomes extract was used in the green synthesis of AgNP. The synthesized AgNP was further characterized through scanning electron microscopy and X-ray diffraction. The AgNP, ranging from 0, 0.025, 0.05, 0.075 or 0.1 mg/l was supplemented in ½ strength liquid MS medium with 6% sucrose and 1 mg/l IBA. The MS medium with 0.05 mg/l AgNP found with significant morphological changes in C. caesia cultures (root number, root length and microrhizomes fresh weight). For the total phenolic and total terpenoids content estimation as well as for antioxidant activity analysis, the extracts of un-treated cultures (6% sucrose + 1 mg/l IBA, without AgNP), AgNP treated cultures (6% sucrose + 1 mg/l IBA with 0.025 and 0.05 mg/l AgNP) was used. The 0.025 and 0.05 mg/l AgNP enhanced the phenolic and terpenoid content in the cultures compared to the field-grown mother plant. The antioxidant activity of the cultures treated with AgNP also increased compared to un-treated cultures and field-grown mother plant. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed that the extract treated with 0.05 mg/l AgNP had increased production of monoterpene (camphor) and sesquiterpenes (β-elemenone and curcumenone). These increased terpenes could be responsible for the enhanced antioxidant activity of C. caesia cultures.
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