萨拉托夫地区流行的 Puumala 病毒(汉坦病毒科:Orthohantavirus)变种的系统发育分析

Yaroslav M. Krasnov, E. V. Naidenova, Natalia P. Guseva, T. Polunina, Natalya A. Sharapova, E. A. Sosedova, Nina V. Kotova, K. S. Zakharov, Andrey V. Kazantsev, I. V. Domanina, V. N. Chekashov, Mikhail M. Shilov, Evgeniy N. Kondratiev, Natalya A. Osina, V. V. Kutyrev
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摘要

目的是确定萨拉托夫地区分离出的普马拉病毒的完整核苷酸序列,并对其基因组变体进行系统发育分析。材料和方法研究样本是 2019 年至 2022 年期间在萨拉托夫州加加林斯基区(原萨拉托夫斯基区)、恩格尔斯基区、新布拉斯基区和赫瓦林斯基区采集的野外材料。为了在样本中特异性地富集普马拉病毒基因组,我们使用了 PCR 技术并开发了特异性引物组。接下来,对得到的 PCR 产物进行测序,并将片段组装成病毒基因组每个片段的一个序列。为了构建系统发生树,我们使用了最大解析算法。结果在萨拉托夫地区分离出的普马拉病毒基因变种彼此具有高度的相似性,这表明它们的起源是一致的。根据系统发育分析,它们在伏尔加河沿岸联邦区其他地区的汉坦病毒所组成的群中形成了一个独立的分支。乌德穆尔共和国和鞑靼斯坦共和国以及萨马拉州和乌里扬诺夫斯克州的病毒变种与萨拉托夫州的样本最为接近。结论所获得的数据表明,菌株明显局限于其携带者的自然生物群落的某些地区或区域。因此,根据这些病毒变种基因组各个片段的序列,可以相当准确地确定患者可能感染的地区和/或病毒携带者的分布情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phylogenetic analysis of variants of the Puumala virus (Hantaviridae: Orthohantavirus) circulating in the Saratov region
The objective is to determine the complete nucleotide sequence and conduct a phylogenetic analysis of genome variants of the Puumala virus isolated in the Saratov region. Materials and methods. The samples for the study were field material collected in the Gagarinsky (formerly Saratovsky), Engelssky, Novoburassky and Khvalynsky districts of the Saratov region in the period from 2019 to 2022. To specifically enrich the Puumala virus genome in the samples, were used PCR and developed a specific primer panel. Next, the resulting PCR products were sequenced and the fragments were assembled into one sequence for each segment of the virus genome. To construct phylogenetic trees, the maximum parsimony algorithm was used. Results. Genetic variants of the Puumala virus isolated in the Saratov region have a high degree of genome similarity to each other, which indicates their unity of origin. According to phylogenetic analysis, they all form a separate branch in the cluster formed by hantaviruses from other subjects of the Volga Federal District. The virus variants from the Republics of Udmurtia and Tatarstan, as well as from the Samara and Ulyanovsk regions, are closest to the samples from the Saratov region. Conclusion. The data obtained show the presence of a pronounced territorial confinement of strains to certain regions or areas that are the natural biotopes of their carriers. This makes it possible to fairly accurately determine the territory of possible infection of patients and/or the circulation of carriers of these virus variants based on the sequence of individual segments of their genome.
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