评估刺槐树皮提取物的体外自由基清除潜力比较

Archana Tiwari, Avinash Tiwari
{"title":"评估刺槐树皮提取物的体外自由基清除潜力比较","authors":"Archana Tiwari, Avinash Tiwari","doi":"10.22159/ajpcr.2024.v17i5.49735","DOIUrl":null,"url":null,"abstract":"Objective: This study was aimed at investigating the comparative 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging potential of different bark extracts of Acacia catechu. The assessment was conducted following concentration-dependent and seasonal-dependent impacts of the same using samples collected in different seasons over 2 successive years.\nMethods: In this, six extracts using ethanol, methanol, aqueous, acetone, chloroform, and benzene solvents were prepared. For the in vitro study, a standard DPPH solution (0.15 mM) was used to check how well the test samples got rid of free radicals. The major biochemical components of test plants, such as quercetin, gallic acid, and catechin, were used as standard drugs.\nResults: Among all test drug concentrations, 31.5–500 μg/mL drug concentrations were observed to be effective, whereas 15.25, 750, and 1000 μg/mL concentrations exerted negligible scavenging effects, and 125 μg/mL concentrations were found to be most effective (p<0.01 or more). The order of scavenging potential of different extracts is seen to be methanolic≥ethanolic>aqueous>acetone>chloroform>benzene. The samples collected during the rainy season were the least effective. Samples collected during the winter and summer seasons, on the other hand, were both more effective (p<0.05) at removing DPPH free radicals.\nConclusion: This study helps to provide primary data on the concentration range, impact of the extraction medium, and sample collected in different seasons. Probably, these findings signify a notable progression in the investigation of the utilization of native plant species for medicinal purposes.","PeriodicalId":504156,"journal":{"name":"Asian Journal of Pharmaceutical and Clinical Research","volume":"1 8","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"EVALUATION OF COMPARATIVE IN VITRO FREE RADICAL SCAVENGING POTENTIAL OF BARK EXTRACTS OF ACACIA CATECHU\",\"authors\":\"Archana Tiwari, Avinash Tiwari\",\"doi\":\"10.22159/ajpcr.2024.v17i5.49735\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: This study was aimed at investigating the comparative 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging potential of different bark extracts of Acacia catechu. The assessment was conducted following concentration-dependent and seasonal-dependent impacts of the same using samples collected in different seasons over 2 successive years.\\nMethods: In this, six extracts using ethanol, methanol, aqueous, acetone, chloroform, and benzene solvents were prepared. For the in vitro study, a standard DPPH solution (0.15 mM) was used to check how well the test samples got rid of free radicals. The major biochemical components of test plants, such as quercetin, gallic acid, and catechin, were used as standard drugs.\\nResults: Among all test drug concentrations, 31.5–500 μg/mL drug concentrations were observed to be effective, whereas 15.25, 750, and 1000 μg/mL concentrations exerted negligible scavenging effects, and 125 μg/mL concentrations were found to be most effective (p<0.01 or more). The order of scavenging potential of different extracts is seen to be methanolic≥ethanolic>aqueous>acetone>chloroform>benzene. The samples collected during the rainy season were the least effective. Samples collected during the winter and summer seasons, on the other hand, were both more effective (p<0.05) at removing DPPH free radicals.\\nConclusion: This study helps to provide primary data on the concentration range, impact of the extraction medium, and sample collected in different seasons. Probably, these findings signify a notable progression in the investigation of the utilization of native plant species for medicinal purposes.\",\"PeriodicalId\":504156,\"journal\":{\"name\":\"Asian Journal of Pharmaceutical and Clinical Research\",\"volume\":\"1 8\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Journal of Pharmaceutical and Clinical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22159/ajpcr.2024.v17i5.49735\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Pharmaceutical and Clinical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22159/ajpcr.2024.v17i5.49735","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

研究目的本研究的目的是调查比较刺槐不同树皮提取物的 1-二苯基-2-苦基肼(DPPH)自由基清除潜力。评估采用连续两年在不同季节采集的样本,根据浓度和季节的影响进行:方法:使用乙醇、甲醇、水、丙酮、氯仿和苯溶剂制备了六种提取物。在体外研究中,使用标准 DPPH 溶液(0.15 mM)来检测测试样本清除自由基的能力。试验植物的主要生化成分,如槲皮素、没食子酸和儿茶素,被用作标准药物:在所有测试药物浓度中,31.5-500 μg/mL 药物浓度的清除效果较好,而 15.25、750 和 1000 μg/mL 药物浓度的清除效果可以忽略不计,125 μg/mL 药物浓度的清除效果最好(水>丙酮>氯仿>苯)。雨季采集的样本效果最差。另一方面,冬季和夏季采集的样本在清除 DPPH 自由基方面都更有效(p<0.05):这项研究有助于提供有关浓度范围、萃取介质的影响以及在不同季节采集的样本的原始数据。这些发现可能标志着在研究本地植物物种的药用价值方面取得了显著进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EVALUATION OF COMPARATIVE IN VITRO FREE RADICAL SCAVENGING POTENTIAL OF BARK EXTRACTS OF ACACIA CATECHU
Objective: This study was aimed at investigating the comparative 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging potential of different bark extracts of Acacia catechu. The assessment was conducted following concentration-dependent and seasonal-dependent impacts of the same using samples collected in different seasons over 2 successive years. Methods: In this, six extracts using ethanol, methanol, aqueous, acetone, chloroform, and benzene solvents were prepared. For the in vitro study, a standard DPPH solution (0.15 mM) was used to check how well the test samples got rid of free radicals. The major biochemical components of test plants, such as quercetin, gallic acid, and catechin, were used as standard drugs. Results: Among all test drug concentrations, 31.5–500 μg/mL drug concentrations were observed to be effective, whereas 15.25, 750, and 1000 μg/mL concentrations exerted negligible scavenging effects, and 125 μg/mL concentrations were found to be most effective (p<0.01 or more). The order of scavenging potential of different extracts is seen to be methanolic≥ethanolic>aqueous>acetone>chloroform>benzene. The samples collected during the rainy season were the least effective. Samples collected during the winter and summer seasons, on the other hand, were both more effective (p<0.05) at removing DPPH free radicals. Conclusion: This study helps to provide primary data on the concentration range, impact of the extraction medium, and sample collected in different seasons. Probably, these findings signify a notable progression in the investigation of the utilization of native plant species for medicinal purposes.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信