聚合物浓度和表面活性剂对谷胱甘肽-卡帕卡拉胶纳米球的物理特性、药物释放和抗氧化活性的影响

Q2 Pharmacology, Toxicology and Pharmaceutics

International Journal of Applied Pharmaceutics Pub Date : 2024-05-07 DOI:10.22159/ijap.2024v16i3.49242

Yuyun Nailufa, Bambang Widjaja

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引用次数: 0

摘要

目的谷胱甘肽是被广泛用作抗衰老和美白剂的抗氧化剂之一。口服 250 毫克/天剂量的谷胱甘肽被证明具有抗衰老作用。同时，谷胱甘肽外用晚霜的剂量为 0.1%，对印尼妇女的皮肤有效。谷胱甘肽是一种抗氧化剂，在储存过程中很容易被氧化。研究目的是优化卡帕卡拉胶聚合物和表面活性剂的浓度，以获得纳米球系统的最佳物理特性，并根据尺寸、PDI、产量、载药量、夹带效率、溶解度和抗氧化活性进行分析：制作纳米球最常用的方法是离子凝胶法，因为事实证明这种方法有效、简便、易于应用。离子凝胶化取决于聚电解质在有反离子存在时交联形成水凝胶珠（通常称为凝胶球）的趋势。纳米球是通过气溶胶离子凝胶技术制备的，然后进行冷冻干燥。该方法使用 0.5% 和 1.0% 的卡拉胶聚合物，并添加表面活性剂作为稳定剂。评估参数包括粒度、夹持效率、药物负载、药物释放和抗氧化活性：对获得的纳米球进行了物理和药物活性测试。成功形成的纳米球粒径为 382.67±52.24 nm，F2 为 325.20±4.62 nm，F3 为 495.39±30.61 nm，F4 为 409.80±4.11 nm。聚合物浓度越大，纳米球的包埋效率和药物含量值越高。纳米球的形态良好，呈球形，表面光滑。谷胱甘肽-Kappa 卡拉胶纳米球的抗氧化活性采用 DPPH 法，结果表明纳米球具有中等或中等偏上的抗氧化活性：最理想的配方是含有 1%卡帕卡拉胶和 0.6%氯化钾的 F4。

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EFFECT OF POLYMER CONCENTRATION AND SURFACTANTS ON PHYSICAL CHARACTERISTICS, DRUG RELEASE AND ANTIOXIDANT ACTIVITY OF GLUTATHIONE-KAPPA CARRAGEENAN NANOSPHERES

Objective: Glutathione is one of the antioxidants widely used as an antiaging and skin lightener. Glutathione at a dose of 250 mg/d orally proved useful as an antiaging. At the same time, glutathione topical night cream is effective at a dose of 0.1% for the skin of Indonesian women. Glutathione is one of the antioxidants that has easily oxidized properties in storage. Research purpose to optimize the concentration of kappa carrageenan polymer and surfactan to obtain the optimal physical characteristics of nanosphere system analyzed based on size, PDI, yield, drug loading, entrapment efficiency, dissolution and antioxidant activity. Methods: The most commonly used method of making nanospheres is ionotropic gelation because it has proven effective, easy, and easy to apply. Ionotropic gelation is depend on the tendency of polyelectrolytes to cross connect to develop hydrogel beads often called gelispheres in the existence of counter ions. Nanospheres were prepared by aerosolization ionotropic gelation technique followed by freeze-drying. This method uses carrageenan polymers of 0.5% and 1.0% with the addition of surfactant as a stabilizer. Evaluation parameters are particle size, entrapment efficiency, drug loading, drug release and antioxidant activity. Results: The results of the nanospheres obtained were tested physically and drug activity. Nanospheres successfully formed, with size 382.67±52.24 nm, F2 325.20±4.62 nm, F3 495.39±30.61 nm, and F4 409.80±4.11 nm. The greater the polymer concentration, the greater the value of entrapment efficiency and drug content in the nanosphere. The morphology of the nanosphere is quite good, spherical, with a smooth surface. The release profile shows that glutathione release is quite good but takes a long time, namely F1 73.91±2.17%, F2 75.91±2.76%, F3 78.56±2.82%, and F4 79.56±1.34% in 480 min or 8 h. Antioxidant activity of glutathione-Kappa carrageenan nanospheres with the DPPH method showed that nanospheres have medium or medium category antioxidant activity. Conclusion: The most optimal formula is F4 with 1% kappa-carrageenan concentration and 0.6% KCl.

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来源期刊

International Journal of Applied Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

219

期刊介绍： International Journal of Applied Pharmaceutics (Int J App Pharm) is a peer-reviewed, bimonthly (onward March 2017) open access journal devoted to the excellence and research in the pure pharmaceutics. This Journal publishes original research work that contributes significantly to further the scientific knowledge in conventional dosage forms, formulation development and characterization, controlled and novel drug delivery, biopharmaceutics, pharmacokinetics, molecular drug design, polymer-based drug delivery, nanotechnology, nanocarrier based drug delivery, novel routes and modes of delivery; responsive delivery systems, prodrug design, development and characterization of the targeted drug delivery systems, ligand carrier interactions etc. However, the other areas which are related to the pharmaceutics are also entertained includes physical pharmacy and API (active pharmaceutical ingredients) analysis. The Journal publishes original research work either as a Original Article or as a Short Communication. Review Articles on a current topic in the said fields are also considered for publication in the Journal.