M. S. Kachurina, L. Zainetdinova, Ye. L. Kurenkov, B. I. Medvedev, T. Shamaeva, L. F. Telesheva, A. Y. Kanaykina
{"title":"褪黑激素水平和核极组织区活性对卵巢子宫内膜异位症妇女不孕症预后的影响","authors":"M. S. Kachurina, L. Zainetdinova, Ye. L. Kurenkov, B. I. Medvedev, T. Shamaeva, L. F. Telesheva, A. Y. Kanaykina","doi":"10.52420/umj.23.2.41","DOIUrl":null,"url":null,"abstract":"Introduction. Endometriosis is a multifactorial disease, affecting about 10 % of women of reproductive age. The purpose of the work is predicting the development of infertility in women with ovarian endometriosis by determining urine melatonin levels and activity indicators of nucleolar organizer regions in endometrioid ovarian heterotopias.Materials and methods. In the Clinic of South Ural State Medical University from 2018 to 2020, 2 groups of women with ovarian endometriosis were studied: group 1 — with infertility (n = 22); group 2 — without it (n = 27). The level of urinary 6-SMT and the activity of ribosomal synthesis were determined.Results. The level of 6-SMT is reduced in patients with stage I ovarian endometriosis. At stagesII and III, the level of 6-SMTis lower in women with infertility than without it: Stage II — (38.82±1.49) ng/ml and (93.04±3.64) ng/ml (p < 0.001); Stage III — (42.84±1.85) ng/ml and (58.06±1.65) ng/ml (p = 0.340). The activity of ribosomal synthesis in epithelial cells of endometrioid heterotopias increases at stage III. In fibroblasts of the stroma of endometrioid heterotopias, the activity of ribosomal synthesis is higherin women with stage III infertility, and in histiocytes— in women with stage II infertility. A logistic stepwise regression analysis was carried out and the most important predictors influencing the development of infertility were identified.Discussion. The decrease in melatonin in women with ovarian endometriosis, suffering from infertility, as well as in stage I ovarian endometriosis is consistent with the research of M. I. Yarmolinskaya et al. (2019). In the presence of infertility, activation of connective tissue histiocytes begins in the early stages. The results obtained may reflect the immunomodulatory effect of melatonin, which is expressed inincreased functional activity of connective tissue histiocytes and fibroblasts.Conclusion. Using mathematical analysis, the most important indicators that influence the reproductive prognosis in women with ovarian endometriosis (activity of nucleolar organizer regions, urine 6-SMT level) were determined, which makes it possible to create a model for calculating an individual reproductive prognosis.","PeriodicalId":247511,"journal":{"name":"Ural Medical Journal","volume":" 45","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Melatonin Level and Activity of Nucleolar Organizer Regions in the Prognosis of Infertility in Women with Ovarian Endometriosis\",\"authors\":\"M. S. Kachurina, L. Zainetdinova, Ye. L. Kurenkov, B. I. Medvedev, T. Shamaeva, L. F. Telesheva, A. Y. Kanaykina\",\"doi\":\"10.52420/umj.23.2.41\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction. Endometriosis is a multifactorial disease, affecting about 10 % of women of reproductive age. The purpose of the work is predicting the development of infertility in women with ovarian endometriosis by determining urine melatonin levels and activity indicators of nucleolar organizer regions in endometrioid ovarian heterotopias.Materials and methods. In the Clinic of South Ural State Medical University from 2018 to 2020, 2 groups of women with ovarian endometriosis were studied: group 1 — with infertility (n = 22); group 2 — without it (n = 27). The level of urinary 6-SMT and the activity of ribosomal synthesis were determined.Results. The level of 6-SMT is reduced in patients with stage I ovarian endometriosis. At stagesII and III, the level of 6-SMTis lower in women with infertility than without it: Stage II — (38.82±1.49) ng/ml and (93.04±3.64) ng/ml (p < 0.001); Stage III — (42.84±1.85) ng/ml and (58.06±1.65) ng/ml (p = 0.340). The activity of ribosomal synthesis in epithelial cells of endometrioid heterotopias increases at stage III. In fibroblasts of the stroma of endometrioid heterotopias, the activity of ribosomal synthesis is higherin women with stage III infertility, and in histiocytes— in women with stage II infertility. A logistic stepwise regression analysis was carried out and the most important predictors influencing the development of infertility were identified.Discussion. The decrease in melatonin in women with ovarian endometriosis, suffering from infertility, as well as in stage I ovarian endometriosis is consistent with the research of M. I. Yarmolinskaya et al. (2019). In the presence of infertility, activation of connective tissue histiocytes begins in the early stages. The results obtained may reflect the immunomodulatory effect of melatonin, which is expressed inincreased functional activity of connective tissue histiocytes and fibroblasts.Conclusion. Using mathematical analysis, the most important indicators that influence the reproductive prognosis in women with ovarian endometriosis (activity of nucleolar organizer regions, urine 6-SMT level) were determined, which makes it possible to create a model for calculating an individual reproductive prognosis.\",\"PeriodicalId\":247511,\"journal\":{\"name\":\"Ural Medical Journal\",\"volume\":\" 45\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ural Medical Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.52420/umj.23.2.41\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ural Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52420/umj.23.2.41","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
简介子宫内膜异位症是一种多因素疾病,约占育龄妇女的10%。这项工作的目的是通过测定尿液中褪黑激素水平和子宫内膜异位症卵巢异位症细胞核组织者区域的活性指标来预测卵巢子宫内膜异位症妇女不孕症的发生。2018年至2020年,南乌拉尔国立医科大学诊所对2组卵巢子宫内膜异位症妇女进行了研究:第1组--不孕(22人);第2组--无不孕(27人)。研究测定了尿液中 6-SMT 的水平和核糖体合成的活性。卵巢子宫内膜异位症 I 期患者的 6-SMT 水平降低。在第二和第三阶段,不孕症妇女的 6-SMT 水平低于无不孕症妇女:II期--(38.82±1.49)纳克/毫升和(93.04±3.64)纳克/毫升(p < 0.001);III期--(42.84±1.85)纳克/毫升和(58.06±1.65)纳克/毫升(p = 0.340)。子宫内膜异位症上皮细胞的核糖体合成活性在 III 期时增加。在子宫内膜异位症基质的成纤维细胞中,第三期不孕妇女的核糖体合成活性较高,而在第二期不孕妇女的组织细胞中,核糖体合成活性较高。通过逻辑逐步回归分析,确定了影响不孕症发展的最重要的预测因素。患有卵巢子宫内膜异位症、不孕的妇女以及I期卵巢子宫内膜异位症患者体内褪黑激素的减少与M. I. Yarmolinskaya等人(2019年)的研究结果一致。在不孕症的早期阶段,结缔组织组织细胞就开始活化。研究结果可能反映了褪黑素的免疫调节作用,这种作用表现为结缔组织组织细胞和成纤维细胞的功能活性增强。通过数学分析,确定了影响卵巢子宫内膜异位症妇女生殖预后的最重要指标(核组织区活性、尿液6-SMT水平),从而建立了计算个体生殖预后的模型。
Melatonin Level and Activity of Nucleolar Organizer Regions in the Prognosis of Infertility in Women with Ovarian Endometriosis
Introduction. Endometriosis is a multifactorial disease, affecting about 10 % of women of reproductive age. The purpose of the work is predicting the development of infertility in women with ovarian endometriosis by determining urine melatonin levels and activity indicators of nucleolar organizer regions in endometrioid ovarian heterotopias.Materials and methods. In the Clinic of South Ural State Medical University from 2018 to 2020, 2 groups of women with ovarian endometriosis were studied: group 1 — with infertility (n = 22); group 2 — without it (n = 27). The level of urinary 6-SMT and the activity of ribosomal synthesis were determined.Results. The level of 6-SMT is reduced in patients with stage I ovarian endometriosis. At stagesII and III, the level of 6-SMTis lower in women with infertility than without it: Stage II — (38.82±1.49) ng/ml and (93.04±3.64) ng/ml (p < 0.001); Stage III — (42.84±1.85) ng/ml and (58.06±1.65) ng/ml (p = 0.340). The activity of ribosomal synthesis in epithelial cells of endometrioid heterotopias increases at stage III. In fibroblasts of the stroma of endometrioid heterotopias, the activity of ribosomal synthesis is higherin women with stage III infertility, and in histiocytes— in women with stage II infertility. A logistic stepwise regression analysis was carried out and the most important predictors influencing the development of infertility were identified.Discussion. The decrease in melatonin in women with ovarian endometriosis, suffering from infertility, as well as in stage I ovarian endometriosis is consistent with the research of M. I. Yarmolinskaya et al. (2019). In the presence of infertility, activation of connective tissue histiocytes begins in the early stages. The results obtained may reflect the immunomodulatory effect of melatonin, which is expressed inincreased functional activity of connective tissue histiocytes and fibroblasts.Conclusion. Using mathematical analysis, the most important indicators that influence the reproductive prognosis in women with ovarian endometriosis (activity of nucleolar organizer regions, urine 6-SMT level) were determined, which makes it possible to create a model for calculating an individual reproductive prognosis.