果糖2,6-二磷酸对大鼠颌下腺磷酸果糖激酶的依赖性调节。

H Sugiya, Y Fujita, E Fukushima, T Yamazaki, S Furuyama
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引用次数: 7

摘要

1. 生理ph值为2时,大鼠颌下腺对磷酸果糖激酶的调节为非michaelis - menten型。在pH 7.3时,ATP在磷酸果糖激酶中发挥双重作用,既是底物,又是高浓度ATP的抑制剂。3.胞浆中存在磷酸果糖激酶活化剂,其性质与果糖2,6-二磷酸相似。4. 激活剂和天然果糖2,6-二磷酸均能解除ATP对磷酸果糖激酶的抑制,增加对果糖6-磷酸的亲和力。5. 果糖2,6-二磷酸在大鼠颌下腺组织中的浓度为8.22 nmol/g,约为肝脏的一半。6. 发现大鼠颌下腺磷酸果糖激酶受ATP、6-磷酸果糖和2,6-二磷酸果糖的协同调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fructose 2,6-bisphosphate-dependent regulation of phosphofructokinase in rat submandibular gland.

1. Regulation of phosphofructokinase in rat submandibular gland was non-Michaelis-Menten type at physiological pH. 2. At pH 7.3, ATP played a dual role on phosphofructokinase acting as a substrate and inhibitor at high concentration of ATP. 3. The activator of phosphofructokinase was present in cytosol fraction, and its properties were resemble to those of fructose 2,6-bisphosphate. 4. Both the activator and authentic fructose 2,6-bisphosphate relieved the inhibition of phosphofructokinase by ATP, and increased the affinity for fructose 6-phosphate. 5. Concentration of fructose 2,6-bisphosphate in rat submandibular gland was 8.22 nmol/g tissue, and which was about the half of that in liver. 6. Phosphofructokinase in rat submandibular gland was found to be regulated synergistically by ATP, fructose 6-phosphate and fructose 2,6-bisphosphate.

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