大胡子龙(Pogona vitticeps)、玉米蛇(Pantherophis guttatus)和林龟(Glyptemys insculpta)血浆中 N-乙酰基-beta-D-葡萄糖苷酶浓度变化的初步结果

Bastien Rubin, Julie Pujol, Pauline Delnatte, Karine Béland, Annabelle Vigneault, C. Gara‐Boivin, Stéphane Lair, Shannon Ferrell, C. Vergneau-Grosset
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摘要

N-acetyl-beta-D-glucosaminidase (NAG)是一种存在于肾小管上皮细胞中的溶酶体酶,其浓度可在血液中测量,作为监测肾脏疾病的一种可能的诊断工具;然而,目前在爬行动物中还没有参考值。本研究调查了 3 种爬行动物的血浆 NAG 浓度。从临床健康的胡须龙(Pogona vitticeps; n = 13)、玉米蛇(Pantherophis guttatus; n = 9)和木龟(Glyptemys insculpta; n = 13)身上采集了血液样本。为进一步评估肾脏健康状况,对所有动物进行了生化检查,并从胡须龙和蛇身上采集了肾活检样本,但没有从乌龟身上采集样本。为了降低对濒危物种进行麻醉的风险,没有对木龟进行活检。在采样的 35 只动物中,有 29 只被认为是健康的,并被纳入研究范围。木龟血浆中的 NAG 浓度明显低于玉米蛇(P = 0.004),玉米蛇血浆中的 NAG 浓度明显高于胡须龙(P = 0.038)。木龟的 NAG 浓度最低,其次是胡须龙和玉米蛇。总之,应为每种爬行动物确定 NAG 参考区间,并且需要对爬行动物中的这种检测方法进行分析验证。要确定 NAG 作为爬行动物诊断工具的可靠性,还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preliminary Results of Variation in Plasma N-acetyl-beta-D-glucosaminidase Concentration Among Bearded Dragons (Pogona vitticeps), Corn Snakes (Pantherophis guttatus), and Wood Turtles (Glyptemys insculpta)
N-acetyl-beta-D-glucosaminidase (NAG) is a lysosomal enzyme found in renal tubular epithelial cells whose concentration can be measured in blood as a possible diagnostic tool to monitor renal disease; however, no reference values are currently available in reptiles. This study investigated the presence of plasma NAG concentrations in 3 reptile species. Blood samples were obtained from clinically healthy bearded dragons (Pogona vitticeps; n = 13), corn snakes (Pantherophis guttatus; n = 9) and wood turtles (Glyptemys insculpta; n = 13). To further assess renal health, a biochemistry panel was performed for all animals, and renal biopsies were collected from the bearded dragons and snakes, but not from the turtles. Biopsies were not taken from wood turtles to limit the risk associated with anesthetizing an endangered species. Of the 35 animals sampled, 29 were considered healthy and included in the study. Plasma NAG concentrations in the wood turtles were significantly lower than in the corn snakes (P = 0.004) and plasma NAG concentrations in the corn snakes were significantly higher than in bearded dragons (P = 0.038). Wood turtles had the lowest NAG concentrations, followed by bearded dragons and corn snakes. In conclusion, NAG reference intervals should be established for each reptile species and analyte validation is needed for this assay in reptiles. Further research is needed to establish NAG reliability as a diagnostic tool in reptiles.
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