在尼日利亚河流州 Eleme 地方政府区 Aleto 河采集的河水样本中检测贾第虫囊蚴和疟原虫卵

S. G. Okere, F.O. Nduka, K. Otokunefor
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引用次数: 0

摘要

本研究旨在检测从尼日利亚里弗州 Eleme 地方政府区 Aleto 河采集的河水样本中的贾第鞭毛虫囊蚴和泰尼亚虫卵,以检查水的可饮用性和纯度。从河水中采集水样时使用了贴有标签、盖子严实的干净样品瓶,并将其储存在装有冰袋的冷却器中,以保持水样的温度并运送到实验室。采集的水样被转移到无菌试管中,以 2000 rpm 的转速离心 5 分钟后,弃去上清液,而沉积物则用抹刀涂抹在贴有标签的载玻片上,用特定染料染色,使用湿装片、毛状体染色技术和改良齐氏-奈尔森技术,并在光学显微镜下观察,以确定寄生虫的存在。这项研究显示,从采集的河水样本中分离出了 10 个蓝氏贾第鞭毛虫滋养体和 10 个疟原虫卵。这些发现凸显了与水传播寄生虫相关的潜在健康风险,并强调了有效的水质管理对保障公众健康的重要性。此外,对寄生虫的观察也强调了对水质进行全面评估和监测的必要性。这项研究凸显了寄生生物对河流的污染,迫切需要有效的水质管理策略来降低对公众健康的风险。重要的干预措施包括加强卫生习惯、遏制粪便污染以及加强水质监测机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Giardia cyst and Taenia eggs in river water samples collected from Aleto River in Eleme Local Government Area, Rivers State, Nigeria
This study aimed at detecting Giardia cyst and Taenia eggs in river water samples collected from Aleto River in Eleme Local Government Area, River State, Nigeria, to check for potability and purity of water.Water samples of river water were collected from two points (downstream and midstream) of Aleto river. Labelled clean sample bottles with tightly-fitted lids were used to collect water samples from the river and stored in coolers with ice packs to maintain their temperature and transportation to the laboratory. Collected water samples were transferred into sterile vial tubes and centrifuged at 2000 rpm for five minutes, after which the supernatant fluids were discarded, while the sediments smeared using spatula on labelled slides stained with specific dyes, using the wet mounts, trichome stain technique, and modified Ziehl–Neelsen techniques, and viewed under the light microscope to identify the presence of parasites.This study showed the presence of ten trophozoites of Giardia lamblia and ten eggs of Taenia species isolated from the river water samples collected. These findings highlight the potential health risks associated with waterborne parasites and emphasizes the importance of effective water quality management to safeguard public health. Furthermore, the observation of parasites underscores the need for comprehensive water quality assessment and monitoring. This study highlights the contamination of the river with parasitic organisms and urgently needs effective water management strategies to mitigate the risks to public health. Vital interventions encompass enhancing sanitation practices, curbing fecal contamination, and augmenting water quality surveillance mechanisms.
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