Ekundare Olugbemi Victor, Fagbuaro Omotayo, Adeniran Idowu Isaac
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引用次数: 0
摘要
Ctenopoma kingsleyae属于Anabantidae科,覆盖的地理区域很广。许多学者对其进行了形态学描述,但缺乏足够的细胞遗传学信息,这对了解其核型进化是一个障碍。因此,本研究描述了来自尼日利亚奥逊州埃萨-奥多水库的 C. kingsleyae(n = 10)的核型。腹腔注射 0.05% 秋水仙素后,从鱼的鳃中获得了染色体的分裂期涂片。用 Giemsa 染色剂对制备的玻片进行染色,以数字方式拍摄分裂期扩散的图像,并制备核型图。研究显示,核型公式为 2n = 48t,单臂染色体。这项研究提供了有关 C. kingsleyae 核型的必要信息,将为进一步研究其进化、遗传资源管理和生产奠定基础。
Preliminary cytogenetic study of Ctenopoma kingsleyae from Esa-Odo water reservoir, Osun State, Nigeria
Ctenopoma kingsleyae belongs to the family Anabantidae covers a wide geographic region. It has been morphologically described by a number of authors, yet it is lacking in adequate cytogenetic information, which is a setback to understanding its karyotypic evolution. This study, therefore, describes the karyotype of C. kingsleyae (n = 10) from Esa-Odo, Reservoir, Osun State, Nigeria. Metaphase chromosome spread was obtained from the gills of fish after intraperitoneal injection with 0.05% colchicines. Slides prepared were stained with Giemsa stain, images of metaphase spread were taken digitally and a karyogram was prepared. The study revealed a karyotypic formula of 2n = 48t with uniarmed chromosomes. This study has provided needful information on C. kingsleyae karyotype and will serve as the basis for further study on its evolution, genetic resource management and production.