通过叶酸共轭氧化石墨烯纳米载体向 HepG2 细胞靶向递送白细胞介素-12 质粒。

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Farshad Safari, Hassan Bardania, Ali Dehshahri, Somayeh Hallaj-Nezhadi, Arash Asfaram, Vahid Mohammadi, Marzieh Baneshi, Sima Bahramianpour, Negar Akrami, Bahman Khalvati, Ali Mirzaei
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引用次数: 0

摘要

成功的基因治疗依赖于载体,以高效、低毒的方式定向转移遗传物质。为了增强细胞的靶向结合和吸收,我们开发并合成了一种基于经支链聚乙烯亚胺(BPEI)和叶酸(FA)修饰的氧化石墨烯(GO)的新型基因递送载体。与未改性的聚乙烯亚胺相比,GO-PEI-FA 纳米载体的毒性更低,同时还具有高效凝结和保护 pDNA 的潜力。有趣的是,增加多聚物配方中的聚合物含量可提高质粒转移能力。在 HepG2 和 THP1 细胞系中,用氧化石墨烯替代 PEI(N/P 比为 10),hIL-12 的表达比单纯的 PEI 提高了约八倍,在相同的 N/P 比下,Hek293 中的表达是 GO-PEI-FA 的两倍。因此,本研究中描述的 GO-PEI-FA 可作为一种靶向纳米载体,将 hIL-12 质粒输送到叶酸受体过表达的细胞中,如肝细胞癌中发现的细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Targeted delivery of interleukin-12 plasmid into HepG2 cells through folic acid conjugated graphene oxide nanocarrier

Successful gene therapy relies on carriers to transfer genetic materials with high efficiency and low toxicity in a targeted manner. To enhance targeted cell binding and uptake, we developed and synthesized a new gene delivery vector based on graphene oxide (GO) modified by branched polyethyleneimine (BPEI) and folic acid (FA). The GO-PEI-FA nanocarriers exhibit lower toxicity compared to unmodified PEI, as well as having the potential to efficiently condense and protect pDNA. Interestingly, increasing the polymer content in the polyplex formulation improved plasmid transfer ability. Substituting graphene oxide for PEI at an N/P ratio of 10 in the HepG2 and THP1 cell lines improved hIL-12 expression by up to approximately eightfold compared to simple PEI, which is twice as high as GO-PEI-FA in Hek293 at the same N/P ratio. Therefore, the GO-PEI-FA described in this study may serve as a targeting nanocarrier for the delivery of the hIL-12 plasmid into cells overexpressing folic acid receptors, such as those found in hepatocellular carcinoma.

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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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