中欧高山是 Huperzia selago(茄科)令人惊讶的细胞型多样性的庇护所

IF 2.6 3区 生物学 Q2 PLANT SCIENCES
Kateřina Vejvodová, Joel Krejčí, Petr Koutecký, Magdalena Lučanová, Ondřej Hornych, Libor Ekrt
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引用次数: 0

摘要

多倍体化是植物物种形成的关键,影响着植物的适应性、生态耐受性和特定的地理分布模式。虽然对被子植物和蕨类植物的细胞型多样性进行了广泛研究,但对同孢球根植物的了解仍然非常有限。我们的研究弥补了这一空白,重点研究了中欧的同孢石蒜科植物Huperzia selago。通过流式细胞仪对来自 208 个种群的 1330 个个体的基因组大小进行了评估,发现了五种不同的细胞型(2x、3x、4x、5x、6x)。使用gemae根进行染色体计数的平均结果为140条(2x)、204条(3x)和262条(4x)染色体。六倍体基因组大小(29 pg)接近狼尾草的上限。四倍体最多(72.7%),三倍体较少(21.3%),其余细胞型在数据集中所占比例不到 5%。没有观察到细胞型的地理分异。不过,不常见的细胞型与阿尔卑斯山有关,三倍体也出现在西喀尔巴阡山脉的最高处。约 27.3% 的种群表现出多种细胞型。除非典型二倍体外,偶倍体(21.8%)和奇倍体(65.2%)的孢子流产率各不相同。微特征(气孔、孢子)的比例与倍性水平的增加不呈线性关系。中欧H. selago的高倍性水平多样性和细胞型共存与蕨类植物和被子植物的模式相吻合。这些发现为研究狼尾草属植物的多倍体性提供了有价值的见解,强调了进行更广泛的地理取样和应用分子研究来阐明狼尾草属的系统发育关系和分类学分类的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

High mountains of central Europe as a refuge of surprising cytotype diversity of Huperzia selago (Lycopodiaceae)

High mountains of central Europe as a refuge of surprising cytotype diversity of Huperzia selago (Lycopodiaceae)

Polyploidization is pivotal in plant speciation, affecting adaptability, ecological tolerance and specific geographical distribution patterns. While cytotype diversity has been extensively studied in angiosperms and ferns, knowledge in homosporous lycophytes remains very limited. Our study addresses this gap, focusing on the homosporous lycophyte Huperzia selago in Central Europe. The genome size of 1330 individuals from 208 populations were assessed via flow cytometry, revealing five distinct cytotypes (2x, 3x, 4x, 5x, 6x). Challenging chromosome counting using gemmae roots yielded average counts of 140 (2x), 204 (3x), and 262 (4x) chromosomes. The hexaploid genome size (29 pg) approached the upper limits reported for lycophytes. Tetraploids were the most abundant (72.7%), while triploids (21.3%) were less common, while the remaining cytotypes comprised less than 5% of the dataset. Geographical separation of cytotypes was not observed. However, uncommon cytotypes were associated with the Alps and triploids also occured in the highest parts of Western Carpathians. Around 27.3% of populations exhibited multiple cytotypes. Except for atypical diploids, spore abortion differed between even (21.8%) and odd ploidies (65.2%). Microcharacter (stoma, spore) proportions did not linearly correspond with increasing ploidy levels. The high ploidy-level diversity and cytotype coexistence in Central European H. selago match the well documented patterns in ferns and angiosperms. These findings provide valuable insights into lycophyte polyploidy, underscoring the necessity for broader geographical sampling and application of molecular studies to elucidate phylogenetic relationships and taxonomic classifications within the genus Huperzia.

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来源期刊
Alpine Botany
Alpine Botany PLANT SCIENCES-
CiteScore
5.10
自引率
18.50%
发文量
15
审稿时长
>12 weeks
期刊介绍: Alpine Botany is an international journal providing a forum for plant science studies at high elevation with links to fungal and microbial ecology, including vegetation and flora of mountain regions worldwide.
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