寒冷海洋环境中养殖的蓝贻贝(Mytilus edulis)的精子发生:三种配子相关分子标记的季节性时空表达

IF 1.3 4区 生物学 Q3 MARINE & FRESHWATER BIOLOGY
Harry M. Murray, Daria Gallardi, Kimberley D. Hobbs
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引用次数: 0

摘要

为了了解寒冷海洋环境中养殖的雄性贻贝精子发生的季节性,我们研究了精子发生早期和后期睾丸顶端生精上皮细胞内发生的细胞转变,具体涉及上皮细胞的组织学、基因特异性生精反应、条件、养殖环境和季节。我们采用组织学评估、qPCR分析和原位杂交相结合的方法,研究了生精上皮细胞从冬末到夏季的季节性转变。我们观察到,组成生精上皮的生精细胞群(即精原干细胞、精母细胞、精子细胞和精子)从二月到七月发生了明显的季节性变化。生精细胞类型的这些季节性变化与两个生精分子标记(即 Kelch 样蛋白 10 [KLHL10]和含犰狳重复蛋白 4 异构体 X2 [ARMC4])的时空表达的显著变化相吻合,但与配子特异性线粒体细胞色素氧化酶 I (MT-COI) 的表达无关。这些基因的时空表达与精子发生过程中生殖上皮细胞的变化直接相关。这些观察结果不仅与生理条件的季节性变化相对应,还与环境温度和叶绿素a的变化相对应,从而进一步证实了高纬度地区雄性配子发生与环境之间的联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Spermatogenesis in cultured blue mussel (Mytilus edulis) from a cold-ocean environment: Seasonal spatiotemporal expression of three gamete-associated molecular markers

Spermatogenesis in cultured blue mussel (Mytilus edulis) from a cold-ocean environment: Seasonal spatiotemporal expression of three gamete-associated molecular markers

To understand the seasonality of spermatogenesis in cultured males of Mytilus edulis from a cold-ocean environment, we investigated the cellular transitions occurring within the spermatogenic epithelium of the testicular acini during early and advancing spermatogenesis, with specific reference to the histology of the epithelium, gene specific spermatogenic response, condition, culture environment, and season. A combination of histological evaluation, qPCR analysis, and in situ hybridization was used to examine the cellular transitions taking place in the germinal epithelium from late winter through to a seasonal spawn in summer. We observed clear seasonal transitionary changes in the spermatogenic cell population making up the germinal epithelium (i.e., spermatogonial stem cells, spermatocytes, spermatids, and spermatozoa) extending from February to July. These seasonal transitions in spermatogenic cell type coincided with significant variation in the spatiotemporal expression of two molecular markers for spermatogenesis (i.e., Kelch-like protein 10 [KLHL10] and Armadillo repeat-containing protein 4 isoform X2 [ARMC4]) but not for expression of a gamete-specific Mitochondrial cytochrome oxidase I (MT-COI). The spatiotemporal expression of these genes is directly linked to the cellular changes taking place in the germinal epithelium during spermatogenesis. These observations not only corresponded to seasonal changes in physiological condition but also environmental temperature and chlorophyll a, thus further supporting the link between male gametogenesis and environment in higher latitude regions.

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来源期刊
Invertebrate Biology
Invertebrate Biology 生物-动物学
CiteScore
2.20
自引率
8.30%
发文量
28
审稿时长
>12 weeks
期刊介绍: Invertebrate Biology presents fundamental advances in our understanding of the structure, function, ecology, and evolution of the invertebrates, which represent the vast majority of animal diversity. Though ultimately organismal in focus, the journal publishes manuscripts addressing phenomena at all levels of biological organization. Invertebrate Biology welcomes manuscripts addressing the biology of invertebrates from diverse perspectives, including those of: • genetics, cell, and molecular biology • morphology and biomechanics • reproduction and development • physiology and behavior • ecology • evolution and phylogenetics
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