Suziane Alves Barcellos, Rafael Kretschmer, Marcelo Santos de Souza, Victoria Tura, Luciano Cesar Pozzobon, Thales Renato Ochotorena de Freitas, Darren K Griffin, Rebecca O'Connor, Ricardo José Gunski, Analía Del Valle Garnero
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Genome organization patterns and hence evolutionary change in the microchromosome formation of four species (<i>Colaptes campestris, Veniliornis spilogaster, Melanerpes candidus</i>, and <i>Picumnus nebulosus)</i> was established through fluorescence in situ hybridization using bacterial artificial chromosomes originally derived from <i>Gallus gallus</i> and <i>Taeniopygia guttata</i>. Findings suggest that <i>P. nebulosus</i> (2<i>n</i> = 110), which was described for the first time, had the most basal karyotype among species of Picidae studied here, and probably arose as a result of fissions of avian ancestral macrochromosomes. We defined a new chromosomal number for <i>V. spilogaster</i> (2<i>n</i> = 88) and demonstrated microchromosomal rearrangements involving <i>C. campestris</i> plus a single, unique hitherto undescribed rearrangement in <i>V. spilogaster</i>. This comprised an inversion after a fusion involving the ancestral microchromosome 12 (homologous to chicken microchromosome 12). We also determined that the low diploid number of <i>M. candidus</i> is related to microchromosome fusions. 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引用次数: 0
摘要
啄木鸟的基因组组织有几个显著特点,如不常见的重复序列积累、增大的 Z 染色体和非典型的二倍体数目。尽管啄木鸟的种类繁多,但详细的细胞基因组研究却很少。通过荧光原位杂交技术,利用最初来源于 Gallus gallus 和 Taeniopygia guttata 的细菌人工染色体,确定了四个物种(Colaptes campestris、Veniliornis spilogaster、Melanerpes candidus 和 Picumnus nebulosus)的基因组组织模式以及微染色体形成过程中的进化变化。研究结果表明,首次描述的 P. nebulosus(2n = 110)在本文研究的鹟科物种中具有最基本的核型,可能是鸟类祖先宏染色体裂解的结果。我们为 V. spilogaster 定义了一个新的染色体数目(2n = 88),并展示了涉及 C. campestris 的微染色体重排,以及 V. spilogaster 中一个迄今未被描述的独特重排。这包括祖先微染色体 12(与鸡微染色体 12 同源)融合后的倒位。我们还确定,M. candidus 的低二倍体数与微染色体融合有关。因此,与推测的祖先核型相比,啄木鸟的核型出现了明显的重新排列。
Understanding microchromosomal organization and evolution in four representative woodpeckers (Picidae, Piciformes) through BAC-FISH analysis.
The genome organization of woodpeckers has several distinctive features e.g., an uncommon accumulation of repetitive sequences, enlarged Z chromosomes, and atypical diploid numbers. Despite the large diversity of species, there is a paucity of detailed cytogenomic studies for this group and we thus aimed to rectify this. Genome organization patterns and hence evolutionary change in the microchromosome formation of four species (Colaptes campestris, Veniliornis spilogaster, Melanerpes candidus, and Picumnus nebulosus) was established through fluorescence in situ hybridization using bacterial artificial chromosomes originally derived from Gallus gallus and Taeniopygia guttata. Findings suggest that P. nebulosus (2n = 110), which was described for the first time, had the most basal karyotype among species of Picidae studied here, and probably arose as a result of fissions of avian ancestral macrochromosomes. We defined a new chromosomal number for V. spilogaster (2n = 88) and demonstrated microchromosomal rearrangements involving C. campestris plus a single, unique hitherto undescribed rearrangement in V. spilogaster. This comprised an inversion after a fusion involving the ancestral microchromosome 12 (homologous to chicken microchromosome 12). We also determined that the low diploid number of M. candidus is related to microchromosome fusions. Woodpeckers thus exhibit significantly rearranged karyotypes compared to the putative ancestral karyotype.
期刊介绍:
Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.