elegans 刺猬相关蛋白是角质层和前角质层的组织和亚结构特异性成分。

IF 3.3 3区 生物学 Q2 GENETICS & HEREDITY
Genetics Pub Date : 2024-08-07 DOI:10.1093/genetics/iyae081
Nicholas D Serra, Chelsea B Darwin, Meera V Sundaram
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引用次数: 0

摘要

在秀丽隐杆线虫中,不同的刺猬相关蛋白和斑块相关蛋白家族的扩大促进了从上皮和感觉器官发育到病原体反应再到蜕皮周期中的角质层脱落等众多过程。由于线虫缺乏典型的刺猬信号通路,这些蛋白的分子功能一直是个谜。在这里,我们发现刺猬相关蛋白是角质层和前角质层顶端细胞外基质的组成成分,这些基质包裹、塑造和保护外部上皮。在成像的四种刺猬相关蛋白中,两种(GRL-2 和 GRL-18)与特定管的角质层有稳定的联系,两种(GRL-7 和 WRT-10)标记了角质层前的亚结构,如沟槽或 alae。我们发现,wrt-10 基因突变会破坏角质层 alae 脊,这与它在基质组织中的结构作用一致。我们推测大多数线虫的刺猬相关蛋白是细胞外基质的顶端成分,这一模型可以解释许多已报道的该家族的功能。这些结果突显了刺猬蛋白与细胞外基质之间古老的联系,并表明线虫刺猬相关蛋白的任何信号作用都将与它们的基质关联密切相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Caenorhabditis elegans Hedgehog-related proteins are tissue- and substructure-specific components of the cuticle and precuticle.

In Caenorhabditis elegans, expanded families of divergent Hedgehog-related and patched-related proteins promote numerous processes ranging from epithelial and sense organ development to pathogen responses to cuticle shedding during the molt cycle. The molecular functions of these proteins have been mysterious since nematodes lack a canonical Hedgehog signaling pathway. Here we show that Hedgehog-related proteins are components of the cuticle and precuticle apical extracellular matrices that coat, shape, and protect external epithelia. Of four Hedgehog-related proteins imaged, two (GRL-2 and GRL-18) stably associated with the cuticles of specific tubes and two (GRL-7 and WRT-10) labeled precuticle substructures such as furrows or alae. We found that wrt-10 mutations disrupt cuticle alae ridges, consistent with a structural role in matrix organization. We hypothesize that most nematode Hedgehog-related proteins are apical extracellular matrix components, a model that could explain many of the reported functions for this family. These results highlight ancient connections between Hedgehog proteins and the extracellular matrix and suggest that any signaling roles of C. elegans Hedgehog-related proteins will be intimately related to their matrix association.

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来源期刊
Genetics
Genetics GENETICS & HEREDITY-
CiteScore
6.90
自引率
6.10%
发文量
177
审稿时长
1.5 months
期刊介绍: GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.
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