大规模转录组和基因组分析揭示了影响鸡胴体性状的新型功能基因 SERPINB6。

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Di Zhao, Ranran Liu, Xiaodong Tan, Huimin Kang, Jie Wang, Zheng Ma, Haiquan Zhao, Hai Xiang, Zhengfen Zhang, Hua Li, Guiping Zhao
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引用次数: 0

摘要

背景胴体性状是肉类生产效率的重要指标。然而,与这些性状相关的分子调控机制仍不清楚:本研究对 399 只天农鹧鸪进行了全面的转录组和基因组分析,以确定与胴体性状相关的关键基因和变异,并阐明其潜在的调控机制。基于弹性网(EN)模型的关联分析,我们确定了12个候选基因(AMY1A、AP3B2、CEBPG、EEF2、EIF4EBP1、FGFR1、FOXD3、GOLM1、LOC107052698、PABPC1、SERPINB6和TBC1D16)与4个胴体相关性状,即活重、胴体重、开膛重和胸肌重有关。通过 EN 模型分析、加权基因共表达网络分析和差异表达分析,SERPINB6 被确定为唯一的重叠基因。细胞水平实验证实,SERPINB6 能促进鸡 DF1 细胞和原代成肌细胞的增殖。进一步的表达全基因组关联研究和关联分析表明,rs317934171是增强SERPINB6表达的关键位点。此外,双荧光素酶报告实验证明,gga-miR-1615 以 SERPINB6 的 3'UTR 为靶点:总之,我们的研究结果表明,SERPINB6 通过促进成纤维细胞和成肌细胞的增殖,成为影响鸡胴体性状的新基因。此外,下游变异 rs317934171 可调节 SERPINB6 的表达。这些结果为鸡胴体性状的分子机制确定了一个新的靶基因和分子标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Large-scale transcriptomic and genomic analyses reveal a novel functional gene SERPINB6 for chicken carcass traits.

Background: Carcass traits are crucial indicators of meat production efficiency. However, the molecular regulatory mechanisms associated with these traits remain unclear.

Results: In this study, we conducted comprehensive transcriptomic and genomic analyses on 399 Tiannong partridge chickens to identify key genes and variants associated with carcass traits and to elucidate the underlying regulatory mechanisms. Based on association analyses with the elastic net (EN) model, we identified 12 candidate genes (AMY1A, AP3B2, CEBPG, EEF2, EIF4EBP1, FGFR1, FOXD3, GOLM1, LOC107052698, PABPC1, SERPINB6 and TBC1D16) for 4 carcass-related traits, namely live weight, dressed weight, eviscerated weight, and breast muscle weight. SERPINB6 was identified as the only overlapping gene by 3 analyses, EN model analysis, weighted gene co-expression network analysis and differential expression analysis. Cell-level experiments confirmed that SERPINB6 promotes the proliferation of chicken DF1 cells and primary myoblasts. Further expression genome-wide association study and association analysis indicated that rs317934171 is the critical site that enhances SERPINB6 expression. Furthermore, a dual-luciferase reporter assay proved that gga-miR-1615 targets the 3'UTR of SERPINB6.

Conclusions: Collectively, our findings reveal that SERPINB6 serves as a novel gene for chicken carcass traits by promoting fibroblast and myoblast proliferation. Additionally, the downstream variant rs317934171 regulates SERPINB6 expression. These results identify a new target gene and molecular marker for the molecular mechanisms of chicken carcass traits.

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CiteScore
10.30
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