化学诱导的 13 线地松鼠锥体退化。

IF 1.1 4区 医学 Q4 NEUROSCIENCES
Hannah M Follett, Emma Warr, Jenna Grieshop, Ching Tzu Yu, Mina Gaffney, Owen R Bowie, Jong Won Lee, Sergey Tarima, Dana K Merriman, Joseph Carroll
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引用次数: 0

摘要

视网膜变性的动物模型对于了解疾病和测试潜在疗法至关重要。诱导变性通常需要施用化学物质,通过破坏新陈代谢途径、信号传导途径或蛋白质合成来杀死光感受器。虽然化学诱导变性已在多种动物(小鼠、大鼠、兔子、猫科动物、13线地松鼠(13-LGS)、猪、小鸡)中得到证实,但很少有研究使用无创高分辨率视网膜成像来监测体内细胞效应。在这里,我们使用纵向扫描光眼底镜(SLO)、光学相干断层扫描和自适应光学 SLO 成像技术,对信噪比大、锥体占优势的 13-LGS (46 只动物,52 只眼睛)进行了研究,以检查在 2019 年和 2020 年整个活动季节静脉注射化学物质后的视网膜结构。我们发现,除了一只眼睛接受了最低浓度的碘乙酸外,其他所有眼睛都诱发了严重的泛视网膜损伤。虽然硝普钠成功诱导了视网膜外层的变性,但结果不一,50% 的对侧对照眼也观察到了损伤。三磷酸腺苷和妥尼霉素诱导视网膜外层特异性损伤的结果各不相同,而注射了硫辛酸的眼睛则没有出现变性迹象。鉴于我们观察到的损伤的差异性,对这种差异性可能的生理根源进行后续研究至关重要。这些额外的研究将进一步推动化学诱导的光感受器变性模型在视锥显性 13-LGS 中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemically induced cone degeneration in the 13-lined ground squirrel.

Animal models of retinal degeneration are critical for understanding disease and testing potential therapies. Inducing degeneration commonly involves the administration of chemicals that kill photoreceptors by disrupting metabolic pathways, signaling pathways, or protein synthesis. While chemically induced degeneration has been demonstrated in a variety of animals (mice, rats, rabbits, felines, 13-lined ground squirrels (13-LGS), pigs, chicks), few studies have used noninvasive high-resolution retinal imaging to monitor the in vivo cellular effects. Here, we used longitudinal scanning light ophthalmoscopy (SLO), optical coherence tomography, and adaptive optics SLO imaging in the euthermic, cone-dominant 13-LGS (46 animals, 52 eyes) to examine retinal structure following intravitreal injections of chemicals, which were previously shown to induce photoreceptor degeneration, throughout the active season of 2019 and 2020. We found that iodoacetic acid induced severe pan-retinal damage in all but one eye, which received the lowest concentration. While sodium nitroprusside successfully induced degeneration of the outer retinal layers, the results were variable, and damage was also observed in 50% of contralateral control eyes. Adenosine triphosphate and tunicamycin induced outer retinal specific damage with varying results, while eyes injected with thapsigargin did not show signs of degeneration. Given the variability of damage we observed, follow-up studies examining the possible physiological origins of this variability are critical. These additional studies should further advance the utility of chemically induced photoreceptor degeneration models in the cone-dominant 13-LGS.

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来源期刊
Visual Neuroscience
Visual Neuroscience 医学-神经科学
CiteScore
2.20
自引率
5.30%
发文量
8
审稿时长
>12 weeks
期刊介绍: Visual Neuroscience is an international journal devoted to the publication of experimental and theoretical research on biological mechanisms of vision. A major goal of publication is to bring together in one journal a broad range of studies that reflect the diversity and originality of all aspects of neuroscience research relating to the visual system. Contributions may address molecular, cellular or systems-level processes in either vertebrate or invertebrate species. The journal publishes work based on a wide range of technical approaches, including molecular genetics, anatomy, physiology, psychophysics and imaging, and utilizing comparative, developmental, theoretical or computational approaches to understand the biology of vision and visuo-motor control. The journal also publishes research seeking to understand disorders of the visual system and strategies for restoring vision. Studies based exclusively on clinical, psychophysiological or behavioral data are welcomed, provided that they address questions concerning neural mechanisms of vision or provide insight into visual dysfunction.
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