Meiry L. Lacerda , Carla D. Lopes , Raphael T.C. Silva , Renan D. Zanetti , Mariete B. Moreira , Adelino V.G. Netto , Wesley A. Souza , Sérgio de Albuquerque , Drielly A. Paixão , Wendell Guerra
{"title":"含有 1,4-苯并二恶烷-6-胺的新型 Pt2+ 复合物：稳定性、DNA 结合以及对人类乳腺癌细胞的抗肿瘤作用","authors":"Meiry L. Lacerda , Carla D. Lopes , Raphael T.C. Silva , Renan D. Zanetti , Mariete B. Moreira , Adelino V.G. Netto , Wesley A. Souza , Sérgio de Albuquerque , Drielly A. Paixão , Wendell Guerra","doi":"10.1016/j.cinorg.2024.100049","DOIUrl":null,"url":null,"abstract":"<div><p>In this work, a new platinum(II) complex was prepared by the reaction of K<sub>2</sub>PtCl<sub>4</sub> with 1,4-benzodioxan-6-amine (bda) at room temperature in the molar ratio 2:1 (L:M). The complex was characterized by physicochemical (elemental analysis, TGA/DTA, and conductivity measurements) and spectroscopic methods (UV–Vis, FTIR, <sup>1</sup>H, <sup>13</sup>C and <sup>195</sup>Pt NMR). According to the spectroscopic data, two bda ligands coordinate to the platinum ion in a monodentate manner through the nitrogen atom to give the complex <em>cis</em>-[PtCl<sub>2</sub>(bda)<sub>2</sub>]. Stability studies were carried out under different conditions and showed that the complex is stable in pure DMF, although it undergoes slow solvolysis in DMSO. The interaction of this complex with calf thymus DNA (CT-DNA) was studied using circular dichroism (CD), electronic absorption and fluorescence spectroscopy. The complex binds to CT-DNA with a K<sub>b</sub> value of 1.06 × 10<sup>3</sup> M<sup>−1</sup> and according to data from CD and fluorescence spectroscopy, appears to interact with DNA by electrostatic forces and or other no intercalative binding mode. Compared to cisplatin, the complex exhibited good cytotoxic activity and selectivity against the MCF-7 tumor cell line, displaying an IC<sub>50</sub> value of 17.33 ± 1.1 μM, as well as inhibited MCF-7 cells migration.</p></div>","PeriodicalId":100233,"journal":{"name":"Chemistry of Inorganic Materials","volume":"3 ","pages":"Article 100049"},"PeriodicalIF":0.0000,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S294974692400017X/pdfft?md5=915058a1b1cdd13f5534c142e9e68456&pid=1-s2.0-S294974692400017X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A novel Pt2+ complex containing 1,4-benzodioxan-6-amine: Stability, DNA binding, and antitumor effects against human breast cancer cells\",\"authors\":\"Meiry L. Lacerda , Carla D. Lopes , Raphael T.C. Silva , Renan D. Zanetti , Mariete B. Moreira , Adelino V.G. Netto , Wesley A. Souza , Sérgio de Albuquerque , Drielly A. Paixão , Wendell Guerra\",\"doi\":\"10.1016/j.cinorg.2024.100049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this work, a new platinum(II) complex was prepared by the reaction of K<sub>2</sub>PtCl<sub>4</sub> with 1,4-benzodioxan-6-amine (bda) at room temperature in the molar ratio 2:1 (L:M). The complex was characterized by physicochemical (elemental analysis, TGA/DTA, and conductivity measurements) and spectroscopic methods (UV–Vis, FTIR, <sup>1</sup>H, <sup>13</sup>C and <sup>195</sup>Pt NMR). According to the spectroscopic data, two bda ligands coordinate to the platinum ion in a monodentate manner through the nitrogen atom to give the complex <em>cis</em>-[PtCl<sub>2</sub>(bda)<sub>2</sub>]. Stability studies were carried out under different conditions and showed that the complex is stable in pure DMF, although it undergoes slow solvolysis in DMSO. The interaction of this complex with calf thymus DNA (CT-DNA) was studied using circular dichroism (CD), electronic absorption and fluorescence spectroscopy. The complex binds to CT-DNA with a K<sub>b</sub> value of 1.06 × 10<sup>3</sup> M<sup>−1</sup> and according to data from CD and fluorescence spectroscopy, appears to interact with DNA by electrostatic forces and or other no intercalative binding mode. Compared to cisplatin, the complex exhibited good cytotoxic activity and selectivity against the MCF-7 tumor cell line, displaying an IC<sub>50</sub> value of 17.33 ± 1.1 μM, as well as inhibited MCF-7 cells migration.</p></div>\",\"PeriodicalId\":100233,\"journal\":{\"name\":\"Chemistry of Inorganic Materials\",\"volume\":\"3 \",\"pages\":\"Article 100049\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S294974692400017X/pdfft?md5=915058a1b1cdd13f5534c142e9e68456&pid=1-s2.0-S294974692400017X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemistry of Inorganic Materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S294974692400017X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry of Inorganic Materials","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S294974692400017X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A novel Pt2+ complex containing 1,4-benzodioxan-6-amine: Stability, DNA binding, and antitumor effects against human breast cancer cells
In this work, a new platinum(II) complex was prepared by the reaction of K2PtCl4 with 1,4-benzodioxan-6-amine (bda) at room temperature in the molar ratio 2:1 (L:M). The complex was characterized by physicochemical (elemental analysis, TGA/DTA, and conductivity measurements) and spectroscopic methods (UV–Vis, FTIR, 1H, 13C and 195Pt NMR). According to the spectroscopic data, two bda ligands coordinate to the platinum ion in a monodentate manner through the nitrogen atom to give the complex cis-[PtCl2(bda)2]. Stability studies were carried out under different conditions and showed that the complex is stable in pure DMF, although it undergoes slow solvolysis in DMSO. The interaction of this complex with calf thymus DNA (CT-DNA) was studied using circular dichroism (CD), electronic absorption and fluorescence spectroscopy. The complex binds to CT-DNA with a Kb value of 1.06 × 103 M−1 and according to data from CD and fluorescence spectroscopy, appears to interact with DNA by electrostatic forces and or other no intercalative binding mode. Compared to cisplatin, the complex exhibited good cytotoxic activity and selectivity against the MCF-7 tumor cell line, displaying an IC50 value of 17.33 ± 1.1 μM, as well as inhibited MCF-7 cells migration.
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