与股骨来源的祖细胞相比,小鼠下颌骨来源的破骨细胞祖细胞在内在特性上存在差异。

IF 3.4 Q2 ENDOCRINOLOGY & METABOLISM
JBMR Plus Pub Date : 2024-03-04 eCollection Date: 2024-05-01 DOI:10.1093/jbmrpl/ziae029
Rachel Clark, Soo Y Park, Elizabeth W Bradley, Kim Mansky, Amy Tasca
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引用次数: 0

摘要

颅面破骨细胞对于牙槽骨吸收、牙齿萌出和正畸牙齿移动等特定部位的过程至关重要。目前对破骨细胞发育和功能的了解大多来自对长骨衍生细胞的研究。对骨骼部位差异的研究很少。本研究的总体目标是确定下颌骨和股骨来源的破骨细胞是否代表不同的群体。为了验证这一假设,我们首先分析了 2 个月大小鼠下颌骨和股骨的骨髓细胞。结果表明,与股骨来源的破骨细胞相比,下颌骨来源的破骨细胞体积(mm2)更大。由于骨髓巨噬细胞是一个异质性群体,我们还对单核细胞进行了筛选,结果表明,与股骨来源的单核细胞相比,下颌骨来源的单核细胞也能形成破骨细胞,且体积增大。我们用流式细胞术分析了两个骨骼部位的破骨细胞前体群。在下颌骨来源的细胞中,新描述的 Ly6CHigh+ 群体和 Ly6Cint 群体均有所增加。单核细胞培养物之间分化潜能的差异表明,Ly6CHigh+群体的增加可能是下颌骨衍生细胞分化潜能增强的原因。与股源性单核细胞相比,下颌源性单核细胞中 Pu.1、C/ebp-a 和 Prdm1 等单核细胞基因的表达量增加。正如预期的那样,随着分化的加强,包括 Nfatc1、Dc-stamp、Ctsk 和 Rank 在内的破骨细胞基因在下颌骨来源的破骨细胞前体中上调。未来的研究将确定下颌骨环境的变化如何导致破骨细胞前体百分比及其分化潜能的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mouse mandibular-derived osteoclast progenitors have differences in intrinsic properties compared with femoral-derived progenitors.

Craniofacial osteoclasts are essential for site-specific processes such as alveolar bone resorption, tooth eruption, and orthodontic tooth movement. Much of the current understanding of osteoclast development and function comes from studies using long bone-derived cells. Minimal investigation has been done to explore skeletal site differences. The overall goal of this study was to determine if mandibular- and femoral-derived osteoclasts represent distinct populations. To test this hypothesis, bone marrow cells were initially analyzed from the mandible and femur of 2-month-old mice. It was shown that mandibular-derived osteoclasts have enhanced size (mm2) compared with femoral-derived osteoclasts. Since bone marrow macrophages are a heterogenous population, we additionally selected for monocytes and demonstrated that mandibular-derived monocytes also form osteoclasts with increased size compared with femoral-derived monocytes. Osteoclast precursor populations from both skeletal sites were analyzed by flow cytometry. A newly described Ly6CHigh+ population as well as the Ly6Cint population was increased in the mandibular-derived cells. The difference in differentiation potential between monocyte cultures suggests that the increase in the Ly6CHigh+ population may explain the enhanced differentiation potential in mandibular-derived cells. Monocyte genes such as Pu.1, C/ebp-a, and Prdm1 are increased in expression in mandibular-derived monocytes compared with femoral-derived monocytes. As expected with enhanced differentiation, osteoclast genes including Nfatc1, Dc-stamp, Ctsk, and Rank are upregulated in mandibular-derived osteoclast precursors. Future studies will determine how changes in the environment of the mandible lead to changes in percentages of osteoclast progenitors and their differentiation potential.

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来源期刊
JBMR Plus
JBMR Plus Medicine-Orthopedics and Sports Medicine
CiteScore
5.80
自引率
2.60%
发文量
103
审稿时长
8 weeks
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