参与 HLA-B27 阳性强直性脊柱炎相关急性前葡萄膜炎的细胞因子和趋化因子。

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2023-12-30 eCollection Date: 2023-01-01

Huan Li, Zhaoxia He, Bolin Deng, Chen Yang, Liang Wang, Jialing Xiao, Weijia Wu, Xiangmei Li, Lixin Zhang, Yutong Wei, Siyu Zhu, Huining Yang, Huanyue Hai, Jiarui Hu, Lin Li, Yi Shi, Man Yu, Ping Shuai, Yuping Liu, Xueming Ju, Gang Wu, Yu Zhou, Jing Zhu, Bo Gong

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引用次数: 0

摘要

目的：急性前葡萄膜炎（AAU）是强直性脊柱炎（AS）最常见的关节外症状。本研究旨在揭示参与人类白细胞抗原（HLA）-B27+ AS相关性AAU免疫发病机制的细胞因子和趋化因子：本研究招募了 21 名 HLA-B27+ AS 相关 AAU 患者和 21 名健康对照（HCs）。所有42名受试者的血清细胞因子浓度均采用中尺度发现（MSD）电化学发光法进行测定。每个样本中测定了 34 种细胞因子、10 种趋化因子、8 种血管生成介质和 4 种血管损伤介质。比较两组细胞因子和趋化因子浓度的差异：结果：HL-B27+强直性脊柱炎相关 AAU 患者血清中 IL-3、TNF-α、IL-6、IL-17D、IL-22、IP10/CXCL10、MIP-3α/CCL20、sFlt-1/VEGFR-1、CRP 和 MCP-4/CCL13 的浓度明显高于 HCs（P < 0.05）。相比之下，HL-B27+ AS相关AAU患者血清中IL-4、IL-8、MIP-1α/CCL3、Eotaxin-3/CCL26、PlGF、VEGF-C和VEGF-D的浓度明显低于HC（P＜0.05）：结论：与 HCs 相比，HLA-B27+AS 相关 AAU 患者血清中多种细胞因子和趋化因子的水平存在显著差异。此外，还发现了一些在其他类型葡萄膜炎中未报道过的新型差异细胞因子和趋化因子。这些结果揭示了HLA-B27+ AS相关性葡萄膜炎的潜在发病机制，可能有助于临床诊断。

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Cytokines and chemokines involved in HLA-B27-positive ankylosing spondylitis-associated acute anterior uveitis.

Purpose: Acute anterior uveitis (AAU) is the most common extra-articular symptom of ankylosing spondylitis (AS). This study aims to reveal the cytokines and chemokines involved in the immunopathogenesis of human leucocyte antigen (HLA)-B27⁺ AS-associated AAU.

Methods: Twenty-one HLA-B27⁺ AS-associated AAU patients and 21 healthy controls (HCs) were recruited for this study. Serum cytokine concentrations in all 42 subjects were determined by the Meso Scale Discovery (MSD) electrochemiluminescence method. In each sample, 34 cytokines, 10 chemokines, eight angiogenesis mediators, and four vascular injury mediators were measured. The differences in cytokine and chemokine concentrations were compared between the two groups.

Results: Concentrations of serum IL-3, TNF-α, IL-6, IL-17D, IL-22, IP10/CXCL10, MIP-3α/CCL20, sFlt-1/VEGFR-1, CRP, and MCP-4/CCL13 were significantly higher in patients with HL-B27⁺ AS-associated AAU than in HCs (p < 0.05). In contrast, concentrations of serum IL-4, IL-8, MIP-1α/CCL3, Eotaxin-3/CCL26, PlGF, VEGF-C, and VEGF-D were significantly lower in patients with HL-B27⁺ AS-associated AAU than in HCs (p < 0.05).

Conclusions: Significant differences were detected in the levels of several cytokines and chemokines in the serum of HLA-B27⁺ AS-associated AAU compared with HCs. Some novel differential cytokines and chemokines that have not been reported in other kinds of uveitis were also identified. These results reveal the underlying pathogenesis of HLA-B27⁺ AS-associated AAU and could potentially aid in clinical diagnosis.

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.