是相关蛋白 1 诱导胰腺干细胞的建立

IF 3.2 4区医学 Q3 CELL & TISSUE ENGINEERING

Cell Transplantation Pub Date : 2024-01-01 DOI:10.1177/09636897241248942

Hirofumi Noguchi, Chika Miyagi-Shiohira, Yoshiki Nakashima, Yuka Onishi, Issei Saitoh, Masami Watanabe

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引用次数: 0

摘要

最近，我们和其他人生成了诱导组织特异性干/祖细胞（iTS/iTP）。与诱导多能干细胞（iPS）相比，iTS/iTP 细胞的优势在于：（1）更容易生成；（2）高效分化；（3）不会形成畸胎瘤。在这项研究中，我们用表达Yes相关蛋白1（YAP）的质粒载体生成了小鼠诱导胰腺干细胞（iTS-P细胞）。iTS-P YAP9细胞表达Foxa2（内胚层标记）和Pdx1（胰腺标记），而Oct3/4和Nanog（胚胎干细胞[ES]标记）在iTS-P YAP9细胞中的表达量明显低于ES细胞。与 ES 细胞相比，iTS-P YAP9 细胞能有效分化成表达胰岛素的细胞。生成自体iTS细胞的能力可应用于再生医学的各种领域。

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Establishment of Induced Pancreatic Stem Cells by Yes-Associated Protein 1.

Recently, we and others generated induced tissue-specific stem/progenitor (iTS/iTP) cells. The advantages of iTS/iTP cells compared with induced pluripotent stem (iPS) cells are (1) easier generation, (2) efficient differentiation, and (3) no teratomas formation. In this study, we generated mouse induced pancreatic stem cells (iTS-P cells) by the plasmid vector expressing Yes-associated protein 1 (YAP). The iTS-P YAP9 cells expressed Foxa2 (endoderm marker) and Pdx1 (pancreatic marker) while the expressions of Oct3/4 and Nanog (marker of embryonic stem [ES] cells) in iTS-P YAP9 cells was significantly lower compared with those in ES cells. The iTS-P YAP9 cells efficiently differentiated into insulin-expressing cells compared with ES cells. The ability to generate autologous iTS cells may be applied to diverse applications of regenerative medicine.

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来源期刊

Cell Transplantation 生物-细胞与组织工程

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

6 months

期刊介绍： Cell Transplantation, The Regenerative Medicine Journal is an open access, peer reviewed journal that is published 12 times annually. Cell Transplantation is a multi-disciplinary forum for publication of articles on cell transplantation and its applications to human diseases. Articles focus on a myriad of topics including the physiological, medical, pre-clinical, tissue engineering, stem cell, and device-oriented aspects of the nervous, endocrine, cardiovascular, and endothelial systems, as well as genetically engineered cells. Cell Transplantation also reports on relevant technological advances, clinical studies, and regulatory considerations related to the implantation of cells into the body in order to provide complete coverage of the field.