Yang Yu, ShiYing Li, Jialin Sun, Yu Wang, LiangZhen Xie, Ying Guo, Jia Li, FengJuan Han
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The migration healing ability of cells was detected using cell scratch assay. Cell migration and invasion were detected using Transwell nesting. TUNEL was applied to detect apoptosis, and ELISA and western blot were used to detect the expression of NF-κB signaling pathway proteins. The pathological changes of the tumor tissues were observed using HE staining in a mouse ovarian cancer xenograft model. Immunofluorescence double staining, RT-PCR, and western blot were used to determine the expression of relevant factors in tumour tissues.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>TRIM44 overexpression promoted the proliferation, migration, and invasion of SKOV3 cells in vitro and inhibited apoptosis while enhancing the growth of tumours in vivo. 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引用次数: 0
摘要
背景卵巢癌(OC)是美国第二大常见癌症,卵巢癌患者通常被诊断为晚期。对卵巢癌的分子机制和潜在治疗靶点的研究正变得日益迫切。本研究探讨了TRIM44的过表达是否介导NF-kB通路以促进OC的进展。方法在SKOV3细胞中构建TRIM44过表达模型,用CCK-8检测细胞的增殖能力。采用细胞划痕试验检测细胞的迁移愈合能力。使用 Transwell 嵌套法检测细胞的迁移和侵袭能力。用 TUNEL 检测细胞凋亡,用 ELISA 和 Western 印迹检测 NF-κB 信号通路蛋白的表达。在小鼠卵巢癌异种移植模型中,采用 HE 染色法观察肿瘤组织的病理变化。结果TRIM44的过表达在体外促进了SKOV3细胞的增殖、迁移和侵袭,在体内抑制了细胞凋亡,同时增强了肿瘤的生长。结论TRIM44过表达可调控NF-κB信号通路,促进OC的进展,TRIM44可能是OC的潜在治疗靶点。
Overexpression of TRIM44 mediates the NF-κB pathway to promote the progression of ovarian cancer
Background
Ovarian cancer (OC) is the second most commonly seen cancer in the US, and patients with OC are commonly diagnosed in the advanced stage. Research into the molecular mechanisms and potential therapeutic targets of OC is becoming increasingly urgent. In our study, we worked to discover the role of TRIM44 in OC development.
Objective
This study explored whether the overexpression of TRIM44 mediates the NF-kB pathway to promote the progression of OC.
Methods
A TRIM44 overexpression model was constructed in SKOV3 cells, and the proliferation ability of the cells was detected using the CCK-8 assay. The migration healing ability of cells was detected using cell scratch assay. Cell migration and invasion were detected using Transwell nesting. TUNEL was applied to detect apoptosis, and ELISA and western blot were used to detect the expression of NF-κB signaling pathway proteins. The pathological changes of the tumor tissues were observed using HE staining in a mouse ovarian cancer xenograft model. Immunofluorescence double staining, RT-PCR, and western blot were used to determine the expression of relevant factors in tumour tissues.
Results
TRIM44 overexpression promoted the proliferation, migration, and invasion of SKOV3 cells in vitro and inhibited apoptosis while enhancing the growth of tumours in vivo. TRIM44 regulated the NF-κB signaling pathway.
Conclusions
TRIM44 overexpression can regulate the NF-κB signaling pathway to promote the progression of OC, and TRIM44 may be a potential therapeutic target for OC.
期刊介绍:
Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.