Zuxia Li , Guigong Geng , Huichun Xie , Lianyu Zhou , Luhao Wang , Feng Qiao
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引用次数: 0
摘要
轮叶草是西藏高原海拔3100-5200米地区的一种传统药用植物。本研究调查了青海河南县（HN）、果洛州（GL）、玉树州（YS）和称多县（CD）的轮叶黑藻中黄酮类代谢物的含量。在河南，山柰酚 3-新橙皮甙、樱草素和生物茶素 A 的含量较高。在 YS 中，柠檬苦素和异槲皮素的含量较高。在 GL 中，ikarisoside A 和 chrysosplenol D 的含量较高。CD 中的沙酮苷、米曲连苷、氯化麦饭石苷和苁蓉苷含量较高。结果表明，59 种黄酮类化合物与 29 个 DEGs 之间存在明显的相关性。11种黄酮类化合物随海拔升高而增加。PAL2、UFGT6、COMT1、HCT2、4CL4和HCT3基因在调节类黄酮的生物合成中起着关键作用。三种酶 CHS、4CL 和 UFGT 在调节黄酮类化合物的生物合成中起着关键作用。这项研究为轮叶黑藻不同地区植物的不同用途提供了生物学和化学证据。
Metabolomic and transcriptomic reveal flavonoid biosynthesis and regulation mechanism in Phlomoides rotata from different habitats
Phlomoides rotata is a traditional medical plant at 3100–5200 m altitude in the Tibet Plateau. In this study, flavonoid metabolites were investigated in P. rotata from Henan County (HN), Guoluo County (GL), Yushu County (YS), and Chengduo County (CD) habitats in Qinghai. The level of kaempferol 3-neohesperidoside, sakuranetin, and biochanin A was high in HN. The content of limocitrin and isoquercetin was high in YS. The levels of ikarisoside A and chrysosplenol D in GL were high. Schaftoside, miquelianin, malvidin chloride, and glabrene in CD exhibited high levels. The results showed a significant correlation between 59 flavonoids and 29 DEGs. Eleven flavonoids increased with altitude. PAL2, UFGT6, COMT1, HCT2, 4CL4, and HCT3 genes were crucial in regulating flavonoid biosynthesis. Three enzymes CHS, 4CL, and UFGT, were crucial in regulating flavonoid biosynthesis. This study provided biological and chemical evidence for the different uses of various regional plants of P. rotata.
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