Kavya Gokul, Raniya Samad, Anne Maria Thomas, Swapna P. Antony
{"title":"揭开珍珠斑（Etroplus suratensis）胸腺素抗菌肽的神秘面纱：分子特征、系统发育分析和功能影响","authors":"Kavya Gokul, Raniya Samad, Anne Maria Thomas, Swapna P. Antony","doi":"10.1016/j.egg.2024.100253","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Antimicrobial peptides are small, cationic, amphipathic peptides composed of 10–50 amino acids, playing a crucial role in innate immunity. While they are found in both vertebrates and invertebrates, they are particularly effective in invertebrates. This study presents the identification and molecular characterization of the potential antimicrobial peptide thymosin from Pearl spot, <em>Etroplus suratensis</em> (<em>Es-Thyβ</em>). Phylogenetic analysis and structural characterization of the identified peptide are analyzed and substantiated through the study.</p></div><div><h3>Results</h3><p>The sequence analysis of <em>Es-Thyβ</em> revealed an Open Reading Frame (ORF) of 132 nucleotides, encoding a 43-amino acid peptide. The hydrophobicity of the peptide, determined using PepDraw, was found to be +68.93 Kcal*mol<sup>−1</sup>. Through <em>in silico</em> analysis the identified Thymosin was determined to be <em>Es-Thyβ</em>. The structure and characteristics of the peptide sequence were further analyzed using multiple <em>in silico</em> software tools, providing confirmation of its identity as <em>Es-Thyβ.</em></p></div><div><h3>Conclusion</h3><p>The study identified and described a potential antimicrobial peptide <em>Es-Thyβ</em>, a member of the thymosin β4 family, with conserved sequences and evolutionary links across teleost fishes.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unveiling thymosin antimicrobial peptide from pearl spot, Etroplus suratensis: Molecular characterization, phylogenetic analysis, and functional implications\",\"authors\":\"Kavya Gokul, Raniya Samad, Anne Maria Thomas, Swapna P. Antony\",\"doi\":\"10.1016/j.egg.2024.100253\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Antimicrobial peptides are small, cationic, amphipathic peptides composed of 10–50 amino acids, playing a crucial role in innate immunity. While they are found in both vertebrates and invertebrates, they are particularly effective in invertebrates. This study presents the identification and molecular characterization of the potential antimicrobial peptide thymosin from Pearl spot, <em>Etroplus suratensis</em> (<em>Es-Thyβ</em>). Phylogenetic analysis and structural characterization of the identified peptide are analyzed and substantiated through the study.</p></div><div><h3>Results</h3><p>The sequence analysis of <em>Es-Thyβ</em> revealed an Open Reading Frame (ORF) of 132 nucleotides, encoding a 43-amino acid peptide. The hydrophobicity of the peptide, determined using PepDraw, was found to be +68.93 Kcal*mol<sup>−1</sup>. Through <em>in silico</em> analysis the identified Thymosin was determined to be <em>Es-Thyβ</em>. The structure and characteristics of the peptide sequence were further analyzed using multiple <em>in silico</em> software tools, providing confirmation of its identity as <em>Es-Thyβ.</em></p></div><div><h3>Conclusion</h3><p>The study identified and described a potential antimicrobial peptide <em>Es-Thyβ</em>, a member of the thymosin β4 family, with conserved sequences and evolutionary links across teleost fishes.</p></div>\",\"PeriodicalId\":37938,\"journal\":{\"name\":\"Ecological Genetics and Genomics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ecological Genetics and Genomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405985424000375\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecological Genetics and Genomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405985424000375","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Unveiling thymosin antimicrobial peptide from pearl spot, Etroplus suratensis: Molecular characterization, phylogenetic analysis, and functional implications
Background
Antimicrobial peptides are small, cationic, amphipathic peptides composed of 10–50 amino acids, playing a crucial role in innate immunity. While they are found in both vertebrates and invertebrates, they are particularly effective in invertebrates. This study presents the identification and molecular characterization of the potential antimicrobial peptide thymosin from Pearl spot, Etroplus suratensis (Es-Thyβ). Phylogenetic analysis and structural characterization of the identified peptide are analyzed and substantiated through the study.
Results
The sequence analysis of Es-Thyβ revealed an Open Reading Frame (ORF) of 132 nucleotides, encoding a 43-amino acid peptide. The hydrophobicity of the peptide, determined using PepDraw, was found to be +68.93 Kcal*mol−1. Through in silico analysis the identified Thymosin was determined to be Es-Thyβ. The structure and characteristics of the peptide sequence were further analyzed using multiple in silico software tools, providing confirmation of its identity as Es-Thyβ.
Conclusion
The study identified and described a potential antimicrobial peptide Es-Thyβ, a member of the thymosin β4 family, with conserved sequences and evolutionary links across teleost fishes.
期刊介绍：
Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms
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