根据辐照小鼠的存活标准比较剂量为 100 毫克/千克的利福霉素(肌苷)和吲哚啉的辐射防护特性

Q4 Medicine
L. A. Romodin, O. Nikitenko, T. M. Bychkova, Yu. A. Zrilova, E. D. Rodionova, D. A. Bocharov
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引用次数: 0

摘要

相关性:由于所有已知的有效辐射防护剂都具有很高的化学毒性,因此研究更安全药物的辐射防护特性非常重要。关于核糖核苷核糖核苷酸(肌苷)的辐射防护特性已有大量研究。不过,目前还没有研究利用辐照动物存活试验,比较核糖核苷酸核糖甙(肌苷)和公认的辐射防护剂(如吲哚啉)的直接辐射防护特性。目的:通过在暴露于外部 X 射线辐射的小鼠中进行存活测试,对核糖酸和吲哚啉的辐射防护特性进行比较评估。材料和方法实验在 200 只 SPF 类雄性 ICR (CD-1) 小鼠身上进行,一式两份。在每次实验中,动物按体重分为以下几组,每组 10 只:活体对照组,不接触药物和辐射;辐射对照组,初步腹腔注射无菌水并接受剂量为 6.0、6.5 和 6.75 Gy 的外部 X 射线辐射;实验组,接受指定剂量的辐射并初步腹腔注射剂量为 100 毫升/千克体重的利巴韦林或剂量为 100 毫升/千克的吲哚啉。对照射后 30 天的存活率进行评估。剂量变化系数采用 probit 分析法确定,即导致一半接受药物照射的动物死亡的辐射剂量与导致一半未接受药物照射的动物死亡的辐射剂量之比。研究结果在剂量为 6.0 Gy、6.5 Gy 和 6.75 Gy 的 X 射线照射前使用吲哚林,与接受利福昔康和对照组相比,动物的存活率在统计学上有显著提高(р<0,05,对数秩检验)。利用 Phinney probit 分析得出的方程,计算出了吲哚啉和利巴韦林的半数致死剂量,由此计算出的剂量变化系数分别为 1.8 和 1.07。结论由于利巴韦林未显示出辐射防护特性,因此在本文所述条件下,通过腹腔给药预防性使用利巴韦林来降低辐射影响可被认为是无效的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of the Radioprotective Properties of Riboxin (Inosine) and Indralin with Prophylactic Administration at Dosages of 100 mg/кg According to the Survival Criterion of Irradiated Mice
Relevance: Due to the high chemical toxicity of all known effective radioprotectors, studies of the radioprotective properties of safer drugs are very relevant. A sufficient number of works are devoted to the radioprotective properties of ribonucleoside riboxin (inosine). However, studies comparing the direct radioprotective properties of riboxin and a recognized radioprotector, for example, indralin, using a survival test in irradiated animals have not yet been carried out. Purpose: Conduct a comparative assessment of the radioprotective properties of riboxin and indralin using a survival test in mice exposed to external X-ray radiation. Material and methods: The experiment was carried out on 200 male ICR (CD-1) mice of the SPF category in duplicate. In each experiment, the animals were divided into the following groups, separated by body weight, 10 animals each: vivar control, not exposed to drugs and radiation, radiation control, with preliminary intraperitoneal administration of sterile water and exposed to external X-ray radiation in doses of 6.0, 6.5 and 6.75 Gy, experimental groups exposed to irradiation in the indicated doses with preliminary intraperitoneal administration of riboxin at a dosage of 100 ml/kg body weight or indralin at a dosage of 100 ml/kg. Survival was assessed for 30 days after irradiation. The dose change factor was determined using probit analysis as the ratio of the radiation dose causing the death of half of the irradiated animals that received the drug to the radiation dose causing the death of half of the irradiated animals without administration of the drug. Results: The use of indralin before X-ray irradiation in doses of 6.0 Gy, 6.5 Gy and 6.75 Gy led to a statistically significant increase in the survival of animals compared to the group receiving Riboxin and control irradiation (р<0,05, log-rank test). Using equations derived from Phinney probit analysis, LD50 doses were calculated for indralin and riboxin, from which dose change factors were calculated to be 1.8 and 1.07, respectively. Conclusion: Since riboxin has not demonstrated radioprotective properties, its preventive use with intraperitoneal administration under the conditions described in this paper, for leveling the effects of radiation can be considered ineffective.
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来源期刊
Medical Radiology and Radiation Safety
Medical Radiology and Radiation Safety Medicine-Radiology, Nuclear Medicine and Imaging
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