急性胰腺炎模型中细胞因子和趋化因子的变化

E. Kınacı, M. Sevinç, Anil Demir, Emre Erdoğan, Fatih Alper Ahlatci, U. Idiz
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摘要

背景急性胰腺炎继发于炎症的免疫反应在疾病的临床过程中起着重要作用。本研究旨在根据胰腺炎的严重程度评估各种细胞因子和趋化因子的变化。对照组不接受任何干预。轻度胰腺炎组和重度胰腺炎组的腹腔注射剂量分别为 50 µg/kg 和 80 µg/kg,每小时一次,连续注射 5 小时。胰腺炎的发展和严重程度在安乐死后通过组织学评估得到确认。从所有大鼠身上抽取血液样本,以测量白细胞介素-10(IL-10)、γ 干扰素(IFN-γ)、C-X-C Motif Chemokine Ligand 1(CXCL-1)、单核细胞趋化蛋白-1(MCP-1)、肿瘤坏死因子-T-1(TNF-γ)、C-X-C Motif Chemokine Ligand 1(CXCL-1)和单核细胞趋化蛋白-1(MCP-1)的水平、肿瘤坏死因子α(TNF-α)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、IL-18、IL-12p70、IL-1β、IL-17A、IL-33、IL-1α 和 IL-6。结果根据组织病理学检查,研究组所有病例均成功诱导出急性胰腺炎模型。研究发现,胰腺炎大鼠的 CXCL-1、MCP-1 和 IL-6 水平在统计学上显著升高,其中重症胰腺炎组的这些参数更高。在相关分析中,MCP-1 和 IL-6 与胰腺炎的严重程度呈中度相关。我们的研究结果突出表明,这些细胞因子的产生和工作途径是治疗干预的潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in cytokines and chemokines in an acute pancreatitis model.
BACKGROUND The immune response secondary to inflammation that develops in acute pancreatitis plays an important role in the clinical course of the disease. This study aims to evaluate the changes in various cytokines and chemokines according to the severity of pancreatitis. METHODS Twenty-one female Wistar albino rats were divided into three equal groups. The control group received no intervention. Intraperitoneal cerulein was administered to the other groups once per hour for five hours at doses of 50 µg/kg and 80 µg/kg for the mild and severe pancreatitis groups, respectively. The development of pancreatitis and its severity level were confirmed by histological evaluation after euthanization. Blood samples were taken from all rats to measure levels of Interleukin-10 (IL-10), Interferon gamma (IFN-γ), C-X-C Motif Chemokine Ligand 1 (CXCL-1), Monocyte Chemoattractant Protein-1 (MCP-1), Tumor Necrosis Factor alpha (TNF-α), Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), IL-18, IL-12p70, IL-1β, IL-17A, IL-33, IL-1α, and IL-6. Additionally, the Schoenberg inflammation scores of pancreatic tissues were evaluated. RESULTS The acute pancreatitis model was successfully induced in all cases within the study groups, according to histopathological examination. It was found that the levels of CXCL-1, MCP-1, and IL-6 were statistically significantly higher in rats with pancreatitis, with these parameters being elevated in the group with severe pancreatitis. In correlation analyses, MCP-1 and IL-6 showed a moderate correlation with the severity of pancreatitis. CONCLUSION CXCL-1, MCP-1, and IL-6 exhibit predictive characteristics for the occurrence and clinical course of pancreatitis. Our results highlight the production and working pathways of these cytokines as potential targets for therapeutic intervention.
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