Van Trinh Toan, Viet Vo Cuong, Van Nguyen Dat, Anh Thi Phan Lan, Ngoc Nguyen Tan, Viet Pham Hung, Anh Thi Phan Lan
{"title":"2020-2022 年在越南北部各省采集的啮齿动物中检测立克次体的种类","authors":"Van Trinh Toan, Viet Vo Cuong, Van Nguyen Dat, Anh Thi Phan Lan, Ngoc Nguyen Tan, Viet Pham Hung, Anh Thi Phan Lan","doi":"10.21055/0370-1069-2024-1-162-167","DOIUrl":null,"url":null,"abstract":"Rickettsiae, which are obligate intracellular bacterial parasites, have a life cycle commonly associated with arthropod vectors and animal hosts. Rodents are recognized as their natural reservoir hosts and play a role in transmitting the bacteria to humans through arthropod vectors. The true extent of rickettsial infections in Vietnam remains underestimated due to a limited understanding of the disease’s epidemiology, resulting in a significant public health burden.The aim of this investigation was to assess the prevalence of Rickettsia in wild-living rodents in Dien Bien, Son La, and Phu Tho provinces of Northern Vietnam between 2020 and 2022.Materials and methods. A total of 396 wild-living rodents were collected from the Northern provinces of Vietnam during 2020–2022. Real-time polymerase chain reaction (PCR) was employed to detect the percentage of Rickettsia-positive specimens. Subsequently, the ompB and 17kDa genes were extracted, amplified, and sequenced from the Rickettsia-positive rodents.Results and discussion. Among the 396 rodents examined, the majority were Rattus norvegicus (56.1%), followed by R. tanezumi (37.4%), R. nitidus (2.5%), and R. germaini (1.8%). R. korodentsensis and R. argentiverter constituted the remaining rodents, accounting for 2.2% of the total. The real-time PCR analysis revealed that 27 blood samples out of the 396 rodent samples collected, tested positive for Rickettsia (6.81%). R. germaini was shown to have the highest positive rate 1/8 (12.5%), followed by R. nitidus 1/10 (10.0%), R. tanezumi 14/149 (9.5%), and R. norvegicus 11/222 (4.9%). No other tick-borne pathogens were detected in any of the provinces. Sequencing of the positive samples as regards the ompB and 17kDa genes revealed the closest relation to R. felis. These findings highlight the potentially high risk of R. felis infection in humans and animals within the studied areas.","PeriodicalId":516710,"journal":{"name":"Problems of Particularly Dangerous Infections","volume":"60 5","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Rickettsia species in rodents collected in the Northern provinces of Vietnam, 2020–2022\",\"authors\":\"Van Trinh Toan, Viet Vo Cuong, Van Nguyen Dat, Anh Thi Phan Lan, Ngoc Nguyen Tan, Viet Pham Hung, Anh Thi Phan Lan\",\"doi\":\"10.21055/0370-1069-2024-1-162-167\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Rickettsiae, which are obligate intracellular bacterial parasites, have a life cycle commonly associated with arthropod vectors and animal hosts. Rodents are recognized as their natural reservoir hosts and play a role in transmitting the bacteria to humans through arthropod vectors. The true extent of rickettsial infections in Vietnam remains underestimated due to a limited understanding of the disease’s epidemiology, resulting in a significant public health burden.The aim of this investigation was to assess the prevalence of Rickettsia in wild-living rodents in Dien Bien, Son La, and Phu Tho provinces of Northern Vietnam between 2020 and 2022.Materials and methods. A total of 396 wild-living rodents were collected from the Northern provinces of Vietnam during 2020–2022. Real-time polymerase chain reaction (PCR) was employed to detect the percentage of Rickettsia-positive specimens. Subsequently, the ompB and 17kDa genes were extracted, amplified, and sequenced from the Rickettsia-positive rodents.Results and discussion. Among the 396 rodents examined, the majority were Rattus norvegicus (56.1%), followed by R. tanezumi (37.4%), R. nitidus (2.5%), and R. germaini (1.8%). R. korodentsensis and R. argentiverter constituted the remaining rodents, accounting for 2.2% of the total. The real-time PCR analysis revealed that 27 blood samples out of the 396 rodent samples collected, tested positive for Rickettsia (6.81%). R. germaini was shown to have the highest positive rate 1/8 (12.5%), followed by R. nitidus 1/10 (10.0%), R. tanezumi 14/149 (9.5%), and R. norvegicus 11/222 (4.9%). No other tick-borne pathogens were detected in any of the provinces. Sequencing of the positive samples as regards the ompB and 17kDa genes revealed the closest relation to R. felis. These findings highlight the potentially high risk of R. felis infection in humans and animals within the studied areas.\",\"PeriodicalId\":516710,\"journal\":{\"name\":\"Problems of Particularly Dangerous Infections\",\"volume\":\"60 5\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Problems of Particularly Dangerous Infections\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21055/0370-1069-2024-1-162-167\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Problems of Particularly Dangerous Infections","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21055/0370-1069-2024-1-162-167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
立克次体是一种细胞内强制性细菌寄生虫,其生命周期通常与节肢动物媒介和动物宿主有关。啮齿动物被认为是立克次体的天然宿主,并通过节肢动物媒介将细菌传播给人类。由于对立克次体疾病流行病学的了解有限,越南立克次体感染的真实程度仍被低估,造成了巨大的公共卫生负担。本调查旨在评估2020年至2022年期间越南北部奠边省、山罗省和富苴省野生啮齿动物中立克次体的流行情况。2020-2022 年期间,在越南北部各省共采集了 396 只野生啮齿动物。采用实时聚合酶链反应(PCR)检测立克次体阳性标本的比例。随后,从立克次体阳性啮齿动物体内提取、扩增并测序了ombB和17kDa基因。在受检的 396 只啮齿类动物中,大多数是诺威格氏鼠类(56.1%),其次是坦兹米立克次体(37.4%)、硝氏立克次体(2.5%)和格尔马尼立克次体(1.8%)。其余的啮齿动物为 R. korodentsensis 和 R. argentiverter,占总数的 2.2%。实时 PCR 分析显示,在采集的 396 个啮齿动物样本中,有 27 个血液样本的立克次体检测呈阳性(6.81%)。其中,R. germaini 的阳性率最高,为 1/8(12.5%),其次是 R. nitidus 1/10(10.0%)、R. tanezumi 14/149(9.5%)和 R. norvegicus 11/222(4.9%)。各省均未检测到其他蜱传病原体。对阳性样本的 ompB 和 17kDa 基因进行测序后发现,它们与 R. felis 的关系最为密切。这些发现突出表明,在所研究的地区,人类和动物感染猫蜱的潜在风险很高。
Detection of Rickettsia species in rodents collected in the Northern provinces of Vietnam, 2020–2022
Rickettsiae, which are obligate intracellular bacterial parasites, have a life cycle commonly associated with arthropod vectors and animal hosts. Rodents are recognized as their natural reservoir hosts and play a role in transmitting the bacteria to humans through arthropod vectors. The true extent of rickettsial infections in Vietnam remains underestimated due to a limited understanding of the disease’s epidemiology, resulting in a significant public health burden.The aim of this investigation was to assess the prevalence of Rickettsia in wild-living rodents in Dien Bien, Son La, and Phu Tho provinces of Northern Vietnam between 2020 and 2022.Materials and methods. A total of 396 wild-living rodents were collected from the Northern provinces of Vietnam during 2020–2022. Real-time polymerase chain reaction (PCR) was employed to detect the percentage of Rickettsia-positive specimens. Subsequently, the ompB and 17kDa genes were extracted, amplified, and sequenced from the Rickettsia-positive rodents.Results and discussion. Among the 396 rodents examined, the majority were Rattus norvegicus (56.1%), followed by R. tanezumi (37.4%), R. nitidus (2.5%), and R. germaini (1.8%). R. korodentsensis and R. argentiverter constituted the remaining rodents, accounting for 2.2% of the total. The real-time PCR analysis revealed that 27 blood samples out of the 396 rodent samples collected, tested positive for Rickettsia (6.81%). R. germaini was shown to have the highest positive rate 1/8 (12.5%), followed by R. nitidus 1/10 (10.0%), R. tanezumi 14/149 (9.5%), and R. norvegicus 11/222 (4.9%). No other tick-borne pathogens were detected in any of the provinces. Sequencing of the positive samples as regards the ompB and 17kDa genes revealed the closest relation to R. felis. These findings highlight the potentially high risk of R. felis infection in humans and animals within the studied areas.