探索脱氧黄酮生物合成：柚皮苷查尔酮是合成异桔梗苷的还原酶的底物。

IF 1.7 4区医学 Q3 PHARMACOLOGY & PHARMACY

Biological & pharmaceutical bulletin Pub Date : 2024-04-06 DOI:10.1248/bpb.b24-00132

Masaaki Shibuya

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引用次数: 0

摘要

Isoliquiritigenin 的形成是脱氧类黄酮生物合成过程中的一个关键反应，由两种酶催化，即查尔酮合成酶（CHS）和还原酶（CHR）。CHS 的底物已经确定。但 CHR 的底物尚不清楚。本研究通过体外反应来确认柚皮苷查尔酮是否可以作为底物。在 CHR 反应中，柚皮苷查尔酮被用作底物。对产物进行分析后发现，柚皮苷查尔酮生成了isisiquiritigenin，这表明柚皮苷查尔酮是一种底物。这项研究首次确定了 CHR 的底物，揭示了脱氧黄酮生物合成从柚皮苷查尔酮分化而来，认可了 "查尔酮还原酶 "这一术语，并回答了长期以来关于脱氧黄酮生物合成中双标记乙酸吸收模式的问题。

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Probing the Deoxyflavonoid Biosynthesis: Naringenin Chalcone Is a Substrate for the Reductase Synthesizing Isoliquiritigenin.

Isoliquiritigenin formation is a key reaction during deoxyflavonoid biosynthesis, which is catalyzed by two enzymes, chalcone synthase (CHS) and reductase (CHR). The substrates for CHS are established. However, the substrate for CHR is unknown. In this study, an in vitro reaction was performed to confirm whether naringenin chalcone can be a substrate. Naringenin chalcone was used as a substrate during the CHR reaction. Analyzing the product revealed that isoliquiritigenin was produced from naringenin chalcone, indicating that naringenin chalcone is a substrate. This study is the first to identify a substrate for CHR, reveals that deoxyflavonoid biosynthesis diverges from naringenin chalcone, endorses the term "chalcone reductase," and answers the long-standing questions about doubly-labeled acetic acid uptake pattern in deoxyflavonoid biosynthesis.

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来源期刊

Biological & pharmaceutical bulletin 医学-药学

CiteScore

3.50

自引率

5.00%

发文量

247

审稿时长

2 months

期刊介绍： Biological and Pharmaceutical Bulletin (Biol. Pharm. Bull.) began publication in 1978 as the Journal of Pharmacobio-Dynamics. It covers various biological topics in the pharmaceutical and health sciences. A fourth Society journal, the Journal of Health Science, was merged with Biol. Pharm. Bull. in 2012. The main aim of the Society’s journals is to advance the pharmaceutical sciences with research reports, information exchange, and high-quality discussion. The average review time for articles submitted to the journals is around one month for first decision. The complete texts of all of the Society’s journals can be freely accessed through J-STAGE. The Society’s editorial committee hopes that the content of its journals will be useful to your research, and also invites you to submit your own work to the journals.