B. M. Aznabayev, L. A. Musina, T. Ismagilov, Timur R. Mukhamadeyev, T. I. Dibayev
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An important aspect of safety of the new method is histological and electron microscopic examination of rabbits’ cornea in different periods after experimental PE. \nAIM: To evaluate histological and electron microscopic features of the rabbits’ cornea after experimental PE using a new method of adaptive control of infusion. \nMATERIALS AND METHODS: The experiment was conducted on 15 eyes of Chinchilla rabbits using a new method of adaptive control of infusion on the basis of Optimed Profi surgical system (Optimedservice, Russia). Histological and electron microscopic examinations of the cornea were performed on the 1st, 7th and 30th day after surgery. \nRESULTS: On the 1st day after surgery, according to the study data, signs of moderate edema of the corneal stroma with mild separation of collagen fiber bundles were observed. In the cell cytoplasm, signs of dystrophic processes in the epithelial cells were noticeable in the form of vacuoles of different sizes. On the 7th day after surgery, signs of alteration in the rabbits’ cornea reduced. In 30th days after surgery, based on the data of histological and electron microscopic examinations, the cornea fully corresponded to norm. \nCONCLUSION: The identified histological and electron microscopic alterations considerably diminish on the 7th day after surgery, and on the 30th day the ultrastructure of the cornea corresponds to the norm.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"4 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultrastructural Features of Rabbits’ Cornea in Phacoemulsification Using New Method of Adaptive Control of Infusion\",\"authors\":\"B. M. Aznabayev, L. A. Musina, T. Ismagilov, Timur R. Mukhamadeyev, T. I. Dibayev\",\"doi\":\"10.17816/pavlovj544169\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"INTRODUCTION: Employees of the Department of Ophthalmology of Bashkir State Medical University, jointly with engineers of the Department of Microsurgical Equipment of Optimedservice, have developed a new method of adaptive control of infusion during phacoemulsification (PE) permitting to control the infusion flow depending on the changes in the rate of aspiration and infusion flows characteristic of a certain hydrodynamic situation, and also on the vacuum level in the aspiration line. An important aspect of safety of the new method is histological and electron microscopic examination of rabbits’ cornea in different periods after experimental PE. \\nAIM: To evaluate histological and electron microscopic features of the rabbits’ cornea after experimental PE using a new method of adaptive control of infusion. \\nMATERIALS AND METHODS: The experiment was conducted on 15 eyes of Chinchilla rabbits using a new method of adaptive control of infusion on the basis of Optimed Profi surgical system (Optimedservice, Russia). Histological and electron microscopic examinations of the cornea were performed on the 1st, 7th and 30th day after surgery. \\nRESULTS: On the 1st day after surgery, according to the study data, signs of moderate edema of the corneal stroma with mild separation of collagen fiber bundles were observed. In the cell cytoplasm, signs of dystrophic processes in the epithelial cells were noticeable in the form of vacuoles of different sizes. On the 7th day after surgery, signs of alteration in the rabbits’ cornea reduced. In 30th days after surgery, based on the data of histological and electron microscopic examinations, the cornea fully corresponded to norm. \\nCONCLUSION: The identified histological and electron microscopic alterations considerably diminish on the 7th day after surgery, and on the 30th day the ultrastructure of the cornea corresponds to the norm.\",\"PeriodicalId\":113364,\"journal\":{\"name\":\"I.P. Pavlov Russian Medical Biological Herald\",\"volume\":\"4 2\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"I.P. Pavlov Russian Medical Biological Herald\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17816/pavlovj544169\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"I.P. Pavlov Russian Medical Biological Herald","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17816/pavlovj544169","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
引言:巴什基尔国立医科大学眼科系的员工与 Optimedservice 公司显微手术设备部的工程师共同开发了一种新的方法,用于在超声乳化(PE)过程中对输液进行自适应控制,该方法可以根据吸液速度和输液流量在某种流体力学情况下的变化以及吸液管道中的真空度来控制输液流量。新方法安全性的一个重要方面是对实验 PE 后不同时期的兔子角膜进行组织学和电子显微镜检查。目的:评估使用自适应控制输液新方法进行实验性 PE 后兔子角膜的组织学和电子显微镜特征。材料与方法:在 Optimed Profi 手术系统(Optimedservice,俄罗斯)的基础上,使用自适应控制输液的新方法对 15 只栗鼠家兔的眼睛进行了实验。术后第 1 天、第 7 天和第 30 天对角膜进行组织学和电子显微镜检查。结果:研究数据显示,术后第 1 天,角膜基质出现中度水肿迹象,胶原纤维束轻度分离。在细胞胞质中,上皮细胞出现了明显的萎缩过程,表现为大小不一的空泡。术后第 7 天,兔子角膜的变化迹象有所减轻。术后第 30 天,根据组织学和电子显微镜检查的数据,角膜完全符合正常水平。结论:术后第 7 天,组织学和电子显微镜检查发现的改变明显减少,第 30 天,角膜的超微结构恢复正常。
Ultrastructural Features of Rabbits’ Cornea in Phacoemulsification Using New Method of Adaptive Control of Infusion
INTRODUCTION: Employees of the Department of Ophthalmology of Bashkir State Medical University, jointly with engineers of the Department of Microsurgical Equipment of Optimedservice, have developed a new method of adaptive control of infusion during phacoemulsification (PE) permitting to control the infusion flow depending on the changes in the rate of aspiration and infusion flows characteristic of a certain hydrodynamic situation, and also on the vacuum level in the aspiration line. An important aspect of safety of the new method is histological and electron microscopic examination of rabbits’ cornea in different periods after experimental PE.
AIM: To evaluate histological and electron microscopic features of the rabbits’ cornea after experimental PE using a new method of adaptive control of infusion.
MATERIALS AND METHODS: The experiment was conducted on 15 eyes of Chinchilla rabbits using a new method of adaptive control of infusion on the basis of Optimed Profi surgical system (Optimedservice, Russia). Histological and electron microscopic examinations of the cornea were performed on the 1st, 7th and 30th day after surgery.
RESULTS: On the 1st day after surgery, according to the study data, signs of moderate edema of the corneal stroma with mild separation of collagen fiber bundles were observed. In the cell cytoplasm, signs of dystrophic processes in the epithelial cells were noticeable in the form of vacuoles of different sizes. On the 7th day after surgery, signs of alteration in the rabbits’ cornea reduced. In 30th days after surgery, based on the data of histological and electron microscopic examinations, the cornea fully corresponded to norm.
CONCLUSION: The identified histological and electron microscopic alterations considerably diminish on the 7th day after surgery, and on the 30th day the ultrastructure of the cornea corresponds to the norm.