K. Dubencovs, A. Suleiko, Anastasija Šuleiko, Elina Didrihsone, Mara Grube, K. Shvirksts, J. Vanags
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The goal of the present research was to develop techniques for increasing the efficiency of PUFA production via C. cohnii cultivation. Multiple experiments were carried out to test and fine-tune the cultivation medium composition and oxygen transfer factors. The biomass yields from individual components, yeast extract, sea salts, and glucose amounted to 5.5, 0.65, and 0.61 g·g−1, respectively. C. cohnii cell susceptibility to mechanical damage was experimentally evaluated. Power inputs of <276.5 W/m3 did not seem to promote cell destruction when Pitched-blade impellers were used. The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. 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The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. 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引用次数: 0
摘要
隐杆线虫(Crypthecodinium cohnii)是一种海洋微藻,可以积累大量的多不饱和脂肪酸(PUFA),从而取代传统的鱼油生产途径。但这些方法会破坏海洋资源,并带来多种下游/提纯问题。将 C. cohnii 用于工业规模生产的主要缺点与低 PUFA 生产率有关。提高 PUFA 合成率的方法之一是在整个培养过程中将培养基成分浓度保持在最佳值,从而提高 PUFA 的生产效率,这样就能成功地将工艺转移到中试和/或工业规模。本研究的目标是开发提高 C. cohnii 生产 PUFA 效率的技术。研究人员进行了多次实验,以测试和微调培养基成分和氧转移因子。单个成分、酵母提取物、海盐和葡萄糖的生物量产量分别为 5.5、0.65 和 0.61 g-g-1。实验评估了 C. cohnii 细胞对机械损伤的敏感性。当使用沟叶叶轮时,小于 276.5 W/m3 的功率输入似乎不会促进细胞破坏。实验表明,所获得的培养条件能有效提高 C. cohnii 的生物量生产率和欧米茄-3 脂肪酸含量。通过使用所应用的方法,最大生物量生产率达到了 8.0 克-升-1-天-1,最高生物量浓度达到了 110 克-升-1。傅立叶变换红外光谱数据显示,在培养过程中,PUFA 的浓度稳步上升。
Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations
Crypthecodinium cohnii is a marine microalga that can accumulate high amounts of polyunsaturated fatty acids (PUFAs) and thus replace conventional routes of fish oil production. They are associated with the destruction of marine resources and multiple downstream/purification complications. The major drawbacks of using C. cohnii for industrial-scale production are associated with low PUFA productivity. One of the means of increasing the PUFA synthesis rate is to maintain the medium component concentrations at optimal values throughout cultivation, thus increasing PUFA production efficiency, which can result in the successful transfer of the process to pilot and/or industrial scale. The goal of the present research was to develop techniques for increasing the efficiency of PUFA production via C. cohnii cultivation. Multiple experiments were carried out to test and fine-tune the cultivation medium composition and oxygen transfer factors. The biomass yields from individual components, yeast extract, sea salts, and glucose amounted to 5.5, 0.65, and 0.61 g·g−1, respectively. C. cohnii cell susceptibility to mechanical damage was experimentally evaluated. Power inputs of <276.5 W/m3 did not seem to promote cell destruction when Pitched-blade impellers were used. The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. A steady increase in the concentration of PUFAs during cultivation was observed from the FTIR data.