[CRISPRi 在结核分枝杆菌生物学中的应用和优化]。

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases Pub Date : 2024-04-12 DOI:10.3760/cma.j.cn112147-20231019-00250

T. H. U. T. H. U. Y. Le, Y. Huang, J. Xie

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引用次数: 0

摘要

由结核分枝杆菌（MTB）感染引起的结核病仍然是一项全球性的公共卫生挑战。耐多药结核病（MDR-TB）和广泛耐药结核病（XDR-TB）菌株使结核病更加难以控制。研究 MTB 生物学的新工具可以发现新的药物靶点。最近，簇状正则交错短回文重复序列干扰（CRISPRi）与下一代测序相结合，为研究 MTB 的生理学和遗传学提供了许多新见解。本综述总结了CRISPRi在MTB生物学中的应用和优化。

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[Application and optimization of CRISPRi to the biology of Mycobacterium tuberculosis].

Tuberculosis, caused by infection with Mycobacterium tuberculosis (MTB), remains a global public health challenge. Multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) strains make tuberculosis more difficult to control. New tools to study the biology of MTB can identify novel targets for drug discovery. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats interference (CRISPRi) combined with next-generation sequencing has provided many novel insights into the physiology and genetics of MTB. This review summarizes the application and optimization of CRISPRi in MTB biology.

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Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases

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