Gustavo Montes-Montes, Román González-Escobedo, L. Muñoz-Castellanos, G. Ávila-Quezada, Obed Ramírez-Sánchez, Alejandra Borrego-Loya, Ismael Ortiz-Aguirre, Zilia Y. Muñoz-Ramírez
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Through ANI results, as well as phylogenomic, pan-, and core-genome analysis, we found that strain LM32 was closely related to the species S. coelicoflavus, while strain LM65 was closely related to the species S. achromogenes subsp. achromogenes. We annotated the functional categories of genes encoded in both strains, which revealed genes involved in nitrogen and phosphorus metabolism. This suggests that these strains have the potential to enhance nutrient availability in the soil, promoting agricultural sustainability. Additionally, we identified gene clusters associated with nitrate and nitrite ammonification, nitrosative stress, allantoin utilization, ammonia assimilation, denitrifying reductase gene clusters, high-affinity phosphate transporter and control of PHO regulon, polyphosphate, and phosphate metabolism. 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引用次数: 0
摘要
农作物根瘤微生物在植物生长和养分循环中起着至关重要的作用。本研究从葡萄根瘤菌中分离了两株链霉菌,分别为 LM32 链霉菌和 LM65 链霉菌,并对其全基因组序列进行了组装、注释和系统进化分析。LM32 链霉菌株的基因组大小为 8.1 Mb,GC 含量为 72.14%;LM65 链霉菌株的基因组大小为 7.3 Mb,GC 含量为 71%。通过 ANI 结果以及系统发生组、泛基因组和核心基因组分析,我们发现菌株 LM32 与 S. coelicoflavus 关系密切,而菌株 LM65 与 S. achromogenes subsp.我们对两株菌株编码基因的功能类别进行了注释,发现了涉及氮和磷代谢的基因。这表明,这些菌株有可能提高土壤中养分的可用性,促进农业的可持续发展。此外,我们还发现了与硝酸盐和亚硝酸盐氨化、亚硝酸盐胁迫、尿囊素利用、氨同化、反硝化还原酶基因簇、高亲和性磷酸盐转运体以及 PHO 调节子、多磷酸盐和磷酸盐代谢控制有关的基因簇。这些发现凸显了这些菌株在可持续农业中的生态作用,特别是在葡萄和其他农作物系统中的作用。
Whole Genome Analysis of Streptomyces spp. Strains Isolated from the Rhizosphere of Vitis vinifera L. Reveals Their Role in Nitrogen and Phosphorus Metabolism
The rhizospheric microorganisms of agricultural crops play a crucial role in plant growth and nutrient cycling. In this study, we isolated two Streptomyces strains, Streptomyces sp. LM32 and Streptomyces sp. LM65, from the rhizosphere of Vitis vinifera L. We then conducted genomic analysis by assembling, annotating, and inferring phylogenomic information from the whole genome sequences. Streptomyces sp. strain LM32 had a genome size of 8.1 Mb and a GC content of 72.14%, while Streptomyces sp. strain LM65 had a genome size of 7.3 Mb and a GC content of 71%. Through ANI results, as well as phylogenomic, pan-, and core-genome analysis, we found that strain LM32 was closely related to the species S. coelicoflavus, while strain LM65 was closely related to the species S. achromogenes subsp. achromogenes. We annotated the functional categories of genes encoded in both strains, which revealed genes involved in nitrogen and phosphorus metabolism. This suggests that these strains have the potential to enhance nutrient availability in the soil, promoting agricultural sustainability. Additionally, we identified gene clusters associated with nitrate and nitrite ammonification, nitrosative stress, allantoin utilization, ammonia assimilation, denitrifying reductase gene clusters, high-affinity phosphate transporter and control of PHO regulon, polyphosphate, and phosphate metabolism. These findings highlight the ecological roles of these strains in sustainable agriculture, particularly in grapevine and other agricultural crop systems.