与俄罗斯肉用美利奴羊脂肪厚度相关的新候选基因

A. Krivoruchko, A. Kanibolotskaya, A. Skokova, O. Yatsyk, P. Sheludko
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引用次数: 0

摘要

利用全基因组关联搜索(全基因组关联研究,GWAS)鉴定与绵羊肉产量相关的候选基因和遗传标记是一个前景广阔的遗传研究领域。我们对俄罗斯肉用美利奴羊腰部脂肪较厚与单核苷酸多态性(SNPs)的相关性进行了全基因组搜索。研究对象是 12 月龄的公羊(n = 50)。使用便携式超声波扫描仪在第1-2腰椎水平测量脂肪密度。使用 Ovine Infinium HD BeadChip 600K DNA 生物芯片对动物进行基因分型。GWAS 的结果是,在俄罗斯肉用美利奴羊中发现了属于 "脂肪厚度 "性状的 14 个 SNPs。大多数 SNP 位于基因间空间。一个 SNP 位于基因外显子,两个 SNP 位于基因内含子。三个置换位于小核 RNA 基因附近,一个位于非编码 RNA 基因附近。根据所分析的基因组中的 SNPs,提出了五个与俄罗斯肉用美利奴羊腰部生长有关的新候选基因:KCNH8、MTERF4、RYBP、NDST3、ENSOARG00000004203。该研究的目标应是研究这些基因的结构,以了解它们对动物表型的作用机制,而鉴定出的 SNPs 可用作育种工作中的新分子标记。
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New candidate genes associated with fat thickness in russian meat merino sheep
Identification of candidate genes and genetic markers associated with meat productivity in sheep using genome-wide association searches (genome-wide association studies, GWAS) is a promising area of genetic research. We conducted a genome-wide search for associations of single nucleotide polymorphisms (SNPs) with thicker fat in the lumbar region in Russian beef Merino sheep. The object of the study was rams aged 12 months (n = 50). Fat density is achieved at the 1st–2nd lumbar spine level using a portable ultrasound scanner. Animal genotyping was done using Ovine Infinium HD BeadChip 600K DNA biochips. As a result of GWAS, 14 SNPs belonging to the trait “fat thickness” were identified in sheep of the Russian meat Merino breed. Most SNPs were located in the intergenic space. One SNP is localized in the exon, and two - in the intron of genes. Three substitutions are near the small nuclear RNA gene and one near the non-coding RNA gene. Based on the analyzed SNPs in the genome, five new candidate genes were proposed that are associated with growth in the lumbar region in Russian beef Merino sheep: KCNH8, MTERF4, RYBP, NDST3, ENSOARG00000004203. The study’s goal should be to study the structure of these genes for the mechanism of their action on animal phenotypes, and the identified SNPs can be used as new molecular markers in breeding work.
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