在硝基蓝四氮唑刺激细胞试验中测试实验性布鲁氏菌病抗原

O. O. Manakova, T. A. Yanchenko, V. S. Vlasenko
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引用次数: 0

摘要

本文介绍了在体外用硝基蓝四氮唑进行刺激活化细胞试验时作为操作者的布鲁氏菌病抗原实验样本的测试结果。在实验室条件下使用了具有不同抗原结构的布鲁氏菌菌株的布鲁氏菌病制剂实验样本。实验研究在布鲁氏菌 R 株免疫的豚鼠身上进行。确定了额外样本的活性和特异性,以及对免疫豚鼠血液中的中性粒细胞具有刺激潜力的最佳浓度。在研究的最初阶段,发现布鲁氏菌会分解。浓度分别为 50 和 100 μg/ml 的尸解抗原在用完整动物血液进行 NBT 试验时不会引起中性粒细胞的非特异性活化。随后,我们使用最佳浓度的布鲁氏菌病抗原实验样本研究了免疫豚鼠在 NBT 试验中的免疫反应动态。研究用生物材料的选择在免疫前和免疫后第 7、14、21、28、42、55、69 和 125 天进行。刺激系数的计算方法是诱导的细胞活性水平与自发的细胞活性水平之比。结果发现,在 NBT 试验中,中性粒细胞特异性活化的最高水平只出现在第 28 天,与血清反应中凝集抗体和补体结合抗体合成的高峰期相对应。通过实验所获得的数据,我们可以得出这样的结论:在与基金会进行 NBT 试验时,可以使用布鲁氏菌病抗原实验样本作为刺激物,以确定在反应形成过程中对机体细胞重组的免疫控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Testing of experimental brucellosis antigens in a stimulated cell test with nitroblue tetrazolium
The results of testing experimental samples of brucellosis antigens as an operator when performing a stimulated activated cell test with nitroblue tetrazolium in vitro are presented. Experimental samples of brucellosis preparations from Brucella strains with different antigenic structures were used in laboratory conditions. Experimental studies were carried out on guinea pigs immunized with Brucella’s R-strain. The activity and specificity of additional samples and the optimal concentration providing the stimulating potential of neutrophils in the blood of immunized guinea pigs were determined. At the first stages of the study, it was noted that Brucella disintegrates. Corpuscular antigens in concentrations were 50 and 100 μg/ml, and this did not cause nonspecific activation of neutrophil granulocytes in the NBT test with the blood of intact animals. Subsequently, we studied the immune response dynamics of immunized guinea pigs in the NBT test using experimental samples of brucellosis antigens in optimal concentrations. The biomaterial selection for research was carried out before immunization and on the 7th, 14th, 21st, 28th, 42nd, 55th, 69th, and 125th day after vaccination. The stimulation coefficient was calculated as the ratio of the induced level of cellular activity to the spontaneous one. It was found that the maximum level of specific activation of neutrophils in the NBT test is characteristic exclusively on the 28th day, corresponding to the peak synthesis of agglutinating and complement-fixing antibodies in serological reactions. The data obtained from the experiment allow us to conclude that it is possible to use experimental samples of brucellosis antigens as a stimulant when performing an NBT test with the Foundation for determining the immune control of cellular restructuring of the body during the formation of the response.
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