U. D'Souza, Chandrashekar K.S., Shruthi R, Vasudev Pai
{"title":"莪术叶提取物生物活性化合物的 GCMS 分析及对实验动物抗帕金森活性的评价","authors":"U. D'Souza, Chandrashekar K.S., Shruthi R, Vasudev Pai","doi":"10.2174/0115734072285993240403110533","DOIUrl":null,"url":null,"abstract":"\n\nCurculigo orchioides Gaertn, a plant belonging to the Hypoxidaceae\nfamily, is well-known for its traditional uses of all plant parts. This plant is commonly known\nas golden eye grass, black musli, Kali musli, or Kali Musali. Parkinson's disease is a neurodegenerative disorder that is commonly observed in older adults. It is caused by a variety of mechanisms, including neuroinflammation, which results in the loss of dopamine release when triggered\nand causes motor symptoms.\n\n\n\nEthanolic extract was prepared by shade-drying the leaves, making them into a coarse\npowder, and the extract was prepared by maceration technique with the help of ethanol. GCMS\nanalysis and Haloperidol-induced Parkinsonism study were carried out to evaluate the antiParkinson activity in albino Wistar rats. Ethanolic extract of Curculigo orchioides was subjected\nto GCMS analysis. The National Institute of Standards and Technology database was used to interpret the mass spectrum of the GC-MS. The screening models were catalepsy, rotarod and a few\nin vitro models, namely the estimation of catalase acetylcholinesterase levels. The animals were\ndivided into 6 groups, namely control, disease, standard (levodopa), low dose (100 mg/kg p.o.),\nmedium dose (200 mg/kg p.o.), high dose (400 mg/kg p.o.) groups having 6 animals each were\nused for rotarod and catalepsy models for 14 days\n\n\n\nThe results obtained showed a dose-dependent increase in the anti-Parkinson activity.\nThe evaluation of neuromuscular movements of the ethanolic leaf extract of Curculigo orchioides\nshowed that the pre-treatment with different ranges of dose (100 mg/ml, 200 mg/kg, and 400\nmg/kg) suggested a dose-dependent significant increase in fall-off time when compared with both\nstandard and disease groups GCMS analysis revealed the presence of flavonoids, Vitamin and\nfatty acid esters.\n\n\n\nThe anti-Parkinson activity of Flavonoids has been reported previously. Hence, the\nactivity may be attributed to kaempferol-3-O-glucuronide.\n","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"GCMS Analysis of Bioactive Compounds and Evaluation of Anti-parkinson\\nActivity of Leaf Extract of Curculigo orchioides in Experimental Animals\",\"authors\":\"U. D'Souza, Chandrashekar K.S., Shruthi R, Vasudev Pai\",\"doi\":\"10.2174/0115734072285993240403110533\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n\\nCurculigo orchioides Gaertn, a plant belonging to the Hypoxidaceae\\nfamily, is well-known for its traditional uses of all plant parts. This plant is commonly known\\nas golden eye grass, black musli, Kali musli, or Kali Musali. Parkinson's disease is a neurodegenerative disorder that is commonly observed in older adults. It is caused by a variety of mechanisms, including neuroinflammation, which results in the loss of dopamine release when triggered\\nand causes motor symptoms.\\n\\n\\n\\nEthanolic extract was prepared by shade-drying the leaves, making them into a coarse\\npowder, and the extract was prepared by maceration technique with the help of ethanol. GCMS\\nanalysis and Haloperidol-induced Parkinsonism study were carried out to evaluate the antiParkinson activity in albino Wistar rats. Ethanolic extract of Curculigo orchioides was subjected\\nto GCMS analysis. The National Institute of Standards and Technology database was used to interpret the mass spectrum of the GC-MS. The screening models were catalepsy, rotarod and a few\\nin vitro models, namely the estimation of catalase acetylcholinesterase levels. The animals were\\ndivided into 6 groups, namely control, disease, standard (levodopa), low dose (100 mg/kg p.o.),\\nmedium dose (200 mg/kg p.o.), high dose (400 mg/kg p.o.) groups having 6 animals each were\\nused for rotarod and catalepsy models for 14 days\\n\\n\\n\\nThe results obtained showed a dose-dependent increase in the anti-Parkinson activity.\\nThe evaluation of neuromuscular movements of the ethanolic leaf extract of Curculigo orchioides\\nshowed that the pre-treatment with different ranges of dose (100 mg/ml, 200 mg/kg, and 400\\nmg/kg) suggested a dose-dependent significant increase in fall-off time when compared with both\\nstandard and disease groups GCMS analysis revealed the presence of flavonoids, Vitamin and\\nfatty acid esters.\\n\\n\\n\\nThe anti-Parkinson activity of Flavonoids has been reported previously. Hence, the\\nactivity may be attributed to kaempferol-3-O-glucuronide.\\n\",\"PeriodicalId\":10772,\"journal\":{\"name\":\"Current Bioactive Compounds\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Bioactive Compounds\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/0115734072285993240403110533\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Bioactive Compounds","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/0115734072285993240403110533","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
GCMS Analysis of Bioactive Compounds and Evaluation of Anti-parkinson
Activity of Leaf Extract of Curculigo orchioides in Experimental Animals
Curculigo orchioides Gaertn, a plant belonging to the Hypoxidaceae
family, is well-known for its traditional uses of all plant parts. This plant is commonly known
as golden eye grass, black musli, Kali musli, or Kali Musali. Parkinson's disease is a neurodegenerative disorder that is commonly observed in older adults. It is caused by a variety of mechanisms, including neuroinflammation, which results in the loss of dopamine release when triggered
and causes motor symptoms.
Ethanolic extract was prepared by shade-drying the leaves, making them into a coarse
powder, and the extract was prepared by maceration technique with the help of ethanol. GCMS
analysis and Haloperidol-induced Parkinsonism study were carried out to evaluate the antiParkinson activity in albino Wistar rats. Ethanolic extract of Curculigo orchioides was subjected
to GCMS analysis. The National Institute of Standards and Technology database was used to interpret the mass spectrum of the GC-MS. The screening models were catalepsy, rotarod and a few
in vitro models, namely the estimation of catalase acetylcholinesterase levels. The animals were
divided into 6 groups, namely control, disease, standard (levodopa), low dose (100 mg/kg p.o.),
medium dose (200 mg/kg p.o.), high dose (400 mg/kg p.o.) groups having 6 animals each were
used for rotarod and catalepsy models for 14 days
The results obtained showed a dose-dependent increase in the anti-Parkinson activity.
The evaluation of neuromuscular movements of the ethanolic leaf extract of Curculigo orchioides
showed that the pre-treatment with different ranges of dose (100 mg/ml, 200 mg/kg, and 400
mg/kg) suggested a dose-dependent significant increase in fall-off time when compared with both
standard and disease groups GCMS analysis revealed the presence of flavonoids, Vitamin and
fatty acid esters.
The anti-Parkinson activity of Flavonoids has been reported previously. Hence, the
activity may be attributed to kaempferol-3-O-glucuronide.
Current Bioactive CompoundsPharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.90
自引率
0.00%
发文量
112
期刊介绍:
The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.