利用 SPME-GC-MS 和化学计量学分析鉴别牛肉、猪肉及其混合物中的挥发性化合物

IF 5.4 3区 材料科学 Q2 CHEMISTRY, PHYSICAL
Z. Ahamed, Jin-kyu Seo, Jeong-Uk Eom, Han-Sul Yang
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引用次数: 0

摘要

27 本研究通过基于化学计量学的方法解决了肉类品种鉴别和掺假 28 这一普遍存在的问题,这对维护公众健康和确保市场公平至关重要 29。采用顶空-固相-微萃取-气相色谱-质谱法(HS-SPME-GC-MS)提取和分析挥发性化合物。31 通过主成分分析(PCA)32 和偏最小二乘法判别分析(PLS-DA),有效识别了掺假肉类样品。通过变量重要性 33 预测(VIP)得分和随机森林测试,11 种关键化合物,包括牛肉中的壬醛、34 十八醛、十六醛、苯甲醛、1-辛醇、己酸、庚酸、辛酸、35 和 2-乙酰基吡咯,以及猪肉中的己醛和 1-辛烯-3-醇,被确定为生物标记物 36。根据 37 掺假比率,这些化合物在掺假样品中呈现出明显的趋势,这在热图中很明显。值得注意的是,脂质降解化合物强烈影响肉类 38 的鉴别。PCA 和 PLS-DA 可显著分离样品,39 前两个成分分别占总方差的 80% 和 72.1%。40 该技术可作为检测熟肉中肉类掺假的可靠方法。41
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Volatile compounds for discrimination between beef, pork, and their admixture using SPME-GC-MS and chemometrics analysis
27 This study addresses the prevalent issue of meat species authentication and adulteration 28 through a chemometrics-based approach, crucial for upholding public health and ensuring a 29 fair marketplace. Volatile compounds were extracted and analyzed using headspace-solid-30 phase-microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). 31 Adulterated meat samples were effectively identified through principal component analysis 32 (PCA) and partial least square-discriminant analysis (PLS-DA). Through variable importance 33 in projection (VIP) scores and a Random Forest test, 11 key compounds, including nonanal, 34 octanal, hexadecanal, benzaldehyde, 1-octanol, hexanoic acid, heptanoic acid, octanoic acid, 35 and 2-acetylpyrrole for beef, and hexanal and 1-octen-3-ol for pork, were robustly identified 36 as biomarkers. These compounds exhibited a discernible trend in adulterated samples based on 37 adulteration ratios, evident in a heatmap. Notably, lipid degradation compounds strongly 38 influenced meat discrimination. PCA and PLS-DA yielded significant sample separation, with 39 the first two components capturing 80% and 72.1% of total variance, respectively. This 40 technique could be a reliable method for detecting meat adulteration in cooked meat. 41
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来源期刊
ACS Applied Energy Materials
ACS Applied Energy Materials Materials Science-Materials Chemistry
CiteScore
10.30
自引率
6.20%
发文量
1368
期刊介绍: ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.
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