新生 BALB/c 小鼠低血糖时脑组织中的细胞腺苷酸能量电荷和腺嘌呤核苷酸

Q4 Biochemistry, Genetics and Molecular Biology
Hassib Narchi, Priyadharshini Yuvaraju, Junu A. George, Richard L. Jayaraj, Radhakrishnan Subramanian
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引用次数: 0

摘要

目的:低血糖发生在新生儿期,但导致细胞水平脑损伤的确切病理生理学尚不清楚。因此,我们利用胰岛素诱导的低血糖建立了一个新生小鼠模型,以分析低血糖在体外对出生后十天内脑核苷酸和腺苷酸能量电荷(AEC)的影响。研究方法使用出生一至十天的 BALB/c 幼崽。在每个年龄组中,六只幼犬接受胰岛素诱导的低血糖治疗,另外六只作为对照组。两组幼鼠均在安乐死后立即采集脑组织。使用液相色谱-串联质谱(LC-MS/MS)分析了低血糖对脑部核苷酸[单磷酸腺苷(AMP)、二磷酸腺苷(ADP)和三磷酸腺苷(ATP)]以及AEC的体外影响。结果显示在 10 天的研究期间,对照组的细胞 AEC 随年龄的增长而稳步下降,降幅至少为 50%(P < 0.05),但顶叶组织除外(P = 0.30),该组织的 AEC 在整个研究期间保持稳定。枕叶组织的下降最为明显(P < 0.001)。在低血糖小鼠中,顶叶组织和枕叶组织中的 AEC 均比对照组明显减少,其中枕叶组织在第 2 天至第 5 天期间减少得更快更明显,从第 5 天起达到极低水平。除枕叶组织外,腺嘌呤核苷酸本身(包括 ATP)都不能反映细胞的 AEC。结论在出生后的头十天,低血糖会逐渐消耗大脑中的细胞腺嘌呤核苷酸,而细胞ATP浓度则不同,它不能适当地反映细胞能量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cellular adenylate energy charge and adenine nucleotides in brain tissue during hypoglycemia in newly born BALB/c mice pups
Aim: Hypoglycemia occurs in the neonatal period but the exact pathophysiology of the resulting brain injury at the cellular level is not well known. Therefore, a neonatal murine model was developed with insulin-induced hypoglycemia, to analyze the in-vitro effects of hypoglycemia on brain nucleotides and adenylate energy charge (AEC) throughout the first ten days of life. Methods: Newly born BALB/c pups between one and ten days of age were used. In each age group, six pups were subjected to insulin-induced hypoglycemia and six others served as controls. In both groups, immediately after euthanasia, brain tissues were collected. The in-vitro effects of hypoglycemia on brain nucleotides [adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP)] were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) as well on AEC. Results: In the controls, the cellular AEC steadily decreased with age by at least 50% over the 10-day study period (P < 0.05) except in the parietal tissue (P = 0.30) where it remained stable throughout that period. The most marked decrease was observed in the occipital tissue (P < 0.001). In the hypoglycemic mice, AEC in both the parietal and occipital tissues decreased significantly more than in the controls, more rapidly and pronounced between day 2 and 5 in the occipital tissue, reaching very low levels from day 5 onward. Except in the occipital tissue, none of the adenine nucleotides on its own, including ATP, reflected the cellular AEC. Conclusions: Over the first ten days of life, hypoglycemia progressively depleted cellular AEC in the brain, unlike cellular ATP concentration which did not appropriately reflect cellular energy.
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CiteScore
2.10
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