微流控芯片上超灵敏检测 hs-cTnI 的电化学发光磁性免疫传感器

IF 2.7
Yun Hui, Zhen Zhao, Weiliang Shu, Fengshan Shen, Weijun Kong, Shengyong Geng, Zhen Xu, Tianzhun Wu, Wenhua Zhou, Xuefeng Yu
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摘要

在复杂的样品基质中对痕量水平的关键生物标记物进行灵敏检测和精确定量已成为一个重要的研究领域。例如,临床指南强烈建议检测高灵敏度心肌肌钙蛋白 I(hs-cTnI),以便早期诊断急性心肌梗死。基于使用单壁碳纳米管(SWCNTs)修饰的电极和掺杂 Ru(bpy)32+ 的二氧化硅纳米粒子(Ru@SiO2)/三丙胺(TPA)系统,开发了一种新型电化学发光(ECL)磁性免疫传感器,用于超灵敏检测 hs-cTnI。在该方法中,大量[Ru(bpy)3]2+被负载在具有高发光效率的二氧化硅纳米颗粒(SiO2)中作为发光体,而 SWCNTs 作为电极表面改性材料对 TPA 具有优异的电氧化能力。随后,通过在电极两端各放置一块磁铁,制作出分层微柱阵列微结构,从而将单层免疫磁性微珠有效地限制在电极表面,使信号增强 7.5 倍。特别是,使用透明的 SWCNT 来修饰透明的 ITO 电极,可实现两个数量级的 ECL 信号放大。在 10 fg/ml 至 10 ng/ml 的宽范围内,hs-cTnI 浓度的线性校准曲线良好,检测限为 8.720 fg/ml(S/N = 3)。这种超灵敏免疫传感器具有卓越的检测性能、稳定性、再现性和选择性。在检测人血清中的 hs-cTnI 时获得了令人满意的回收率,为临床应用提供了一种潜在的分析方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip
Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of research. For example, the detection of high-sensitivity cardiac troponin I (hs-cTnI) is strongly recommended in clinical guidelines for early diagnosis of acute myocardial infarction. Based on the use of an electrode modified by single-walled carbon nanotubes (SWCNTs) and a Ru(bpy)32+-doped silica nanoparticle (Ru@SiO2)/tripropylamine (TPA) system, a novel type of electrochemiluminescent (ECL) magneto-immunosensor is developed for ultrasensitive detection of hs-cTnI. In this approach, a large amount of [Ru(bpy)3]2+ is loaded in SiO2 (silica nanoparticles) as luminophores with high luminescent efficiency and SWCNTs as electrode surface modification material with excellent electrooxidation ability for TPA. Subsequently, a hierarchical micropillar array of microstructures is fabricated with a magnet placed at each end to efficiently confine a single layer of immunomagnetic microbeads on the surface of the electrode and enable 7.5-fold signal enhancement. In particular, the use of transparent SWCNTs to modify a transparent ITO electrode provides a two-order-of-magnitude ECL signal amplification. A good linear calibration curve is developed for hs-cTnI concentrations over a wide range from 10 fg/ml to 10 ng/ml, with the limit of detection calculated as 8.720 fg/ml (S/N = 3). This ultrasensitive immunosensor exhibits superior detection performance with remarkable stability, reproducibility, and selectivity. Satisfactory recoveries are obtained in the detection of hs-cTnI in human serum, providing a potential analysis protocol for clinical applications.
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