轴向加载能否恢复体内椎间盘的几何形状、开放压力和T2弛豫时间?

IF 3.4 3区 医学 Q1 ORTHOPEDICS
JOR Spine Pub Date : 2024-04-25 DOI:10.1002/jsp2.1322
Harrah R. Newman, Axel C. Moore, Kyle D. Meadows, Rachel L. Hilliard, Madeline S. Boyes, Edward J. Vresilovic, Thomas P. Schaer, Dawn M. Elliott
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引用次数: 0

摘要

背景 尸体椎间盘经常被用来研究各种研究问题,研究结果根据活体情况进行解释。遗憾的是,尸体椎间盘本质上并不代表活体状态,因此在尸体上测量的椎间盘结构(几何形状)、组成(T2弛豫时间)和机械功能(打开压力,OP)并不一定代表活体椎间盘。 方法 我们在尤卡坦小型猪体内对椎间盘的几何形状、T2弛豫时间和OP进行了连续评估,以量化进行性解剖时发生的变化,并使用轴向加载来恢复体内状态。 结果 我们发现,在任何参数上,LIVE 和 TORSO 都没有差异;因此,在牺牲后 2 小时内,TORSO 椎间盘可以代表 LIVE 状态。通过连续解剖和样本制备,椎间盘高度增加(SEGMENT高度比TORSO高18%),OP减少(POTTED比TORSO低67%),T2时间不变。在轴向加载时,0.20-0.33 兆帕的外加应力可使椎间盘恢复到体内状态、LIVE 椎间盘几何形状和 OP,但 T2 时间有所缩短。施加的应力与OP之间呈线性相关,这在多项研究和物种中都是一致的。 结论 为了在人体研究或其他动物模型中恢复椎间盘的活体状态,我们建议测量 OP/应力关系,并利用这种关系选择恢复活体状态所需的外加应力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Can axial loading restore in vivo disc geometry, opening pressure, and T2 relaxation time?

Can axial loading restore in vivo disc geometry, opening pressure, and T2 relaxation time?

Background

Cadaveric intervertebral discs are often studied for a variety of research questions, and outcomes are interpreted in the in vivo context. Unfortunately, the cadaveric disc does not inherently represent the LIVE condition, such that the disc structure (geometry), composition (T2 relaxation time), and mechanical function (opening pressure, OP) measured in the cadaver do not necessarily represent the in vivo disc.

Methods

We conducted serial evaluations in the Yucatan minipig of disc geometry, T2 relaxation time, and OP to quantify the changes that occur with progressive dissection and used axial loading to restore the in vivo condition.

Results

We found no difference in any parameter from LIVE to TORSO; thus, within 2 h of sacrifice, the TORSO disc can represent the LIVE condition. With serial dissection and sample preparation the disc height increased (SEGMENT height 18% higher than TORSO), OP decreased (POTTED was 67% lower than TORSO), and T2 time was unchanged. With axial loading, an imposed stress of 0.20–0.33 MPa returned the disc to in vivo, LIVE disc geometry and OP, although T2 time was decreased. There was a linear correlation between applied stress and OP, and this was conserved across multiple studies and species.

Conclusion

To restore the LIVE disc state in human studies or other animal models, we recommend measuring the OP/stress relationship and using this relationship to select the applied stress necessary to recover the in vivo condition.

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来源期刊
JOR Spine
JOR Spine ORTHOPEDICS-
CiteScore
6.40
自引率
18.90%
发文量
42
审稿时长
10 weeks
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