侵袭性乳腺癌患者体内 CXCL8/CXCR1/R2 信号轴的临床意义。

IF 2.5 4区医学 Q3 ONCOLOGY

Oncology Letters Pub Date : 2024-04-10 DOI:10.3892/ol.2024.14393

Sebastian Stępień, Marta Smycz-Kubańska, Celina Kruszniewska-Rajs, Joanna Magdalena Gola, Jacek Kabut, Paweł Olczyk, Aleksandra Mielczarek-Palacz

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引用次数: 0

摘要

C-X-C motif趋化因子配体8（CXCL8）-C-X-C趋化因子受体（CXCR）1/2信号轴是刺激免疫系统抵御肿瘤生长并影响肿瘤微环境以促进肿瘤生长的众多机制之一。这一通路在包括乳腺癌（BC）在内的多种癌症的发病过程中发挥着重要作用。本研究的目的是分析浸润性乳腺癌女性患者血清中趋化因子CXCL8及其受体CXCR1和CXCR2的水平，并评估这些参数在mRNA水平上的表达，同时考虑到癌症的分子亚型和恶性程度。研究组由 62 名经组织病理学证实的浸润性 BC 患者组成。对照组包括18名经组织病理学确诊的乳腺纤维腺瘤（一种良性乳腺肿瘤）患者。CXCL8、CXCR1和CXCR2的水平采用夹心ELISA法测定，使用的是CLOUD-CLONE ELISA试剂盒。使用反转录定量 PCR 分析了 CXCL8、CXCR1 和 CXCR2 的转录水平。结果显示，女性浸润性BC患者血清中的CXCL8水平明显高于对照组（P<0.05）。此外，与对照组相比，在管腔 B 型人表皮生长因子受体 2+ 癌中观察到 CXCR1 水平明显升高。对女性 BC 患者血清中 CXCL8 的分析表明，临床分期 G1 和 G2（P<0.05）、G2 和 G3（P<0.01）以及 G1 和 G3（P<0.0001）之间的差异有统计学意义。另一方面，对患者血清中 CXCR1 和 CXCR2 水平的分析表明，G2 和 G3 之间的差异具有统计学意义（P<0.05）。目前的研究表明，侵袭性 BC 患者涉及 CXCL8-CXCR1/2 信号轴的免疫反应异常与这些肿瘤的发展有关。此外，蛋白质水平变化的严重程度表明，将其测定为潜在的诊断标记物在临床上可能很有用。

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Clinical significance of the CXCL8/CXCR1/R2 signalling axis in patients with invasive breast cancer.

The C-X-C motif chemokine ligand 8 (CXCL8)-C-X-C chemokine receptor (CXCR)1/2 signalling axis is among numerous mechanisms which stimulate the immune system to defend against tumour growth and influence the tumour microenvironment to promote tumour growth. This pathway plays an important role in the development of a number of cancers including breast cancer (BC). The aim of the present study was to analyse the levels of the chemokine CXCL8 and its receptors, CXCR1 and CXCR2, in the serum of female patients with invasive BC and to assess the expression of these parameters at the mRNA level, considering molecular subtypes and degrees of cancer malignancy. The study group consisted of 62 patients with histopathologically confirmed invasive BC. The control group consisted of 18 patients with histopathologically confirmed fibroadenoma, a benign breast tumour. The levels of CXCL8, CXCR1 and CXCR2 were determined by sandwich ELISA using the CLOUD-CLONE ELISA kit. CXCL8, CXCR1 and CXCR2 transcript levels were analysed using reverse transcription-quantitative PCR. Results showed that serum CXCL8 levels in female patients with invasive BC were significantly higher compared with those in the control group (P<0.05). In addition, significantly elevated CXCR1 levels were observed in luminal B human epidermal growth factor receptor 2⁺ carcinoma compared with those in the control group. Analysis of CXCL8 in the serum of female patients with BC showed a statistically significant difference between clinical stage G1 and G2 (P<0.05), G2 and G3 (P<0.01), and G1 and G3 (P<0.0001). On the other hand, the analysis of CXCR1 and CXCR2 levels in the serum of the patients revealed a statistically significant difference between G2 and G3 (P<0.05). The current study showed that abnormalities in the immune response involving the CXCL8-CXCR1/2 signalling axis in patients with invasive BC are involved in the development of these tumours. Moreover, the demonstrated severity of changes occurring at protein level may suggest the potential usefulness of their determination as potential diagnostic markers in the clinic.

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来源期刊

Oncology Letters ONCOLOGY-

CiteScore

5.70

自引率

0.00%

发文量

412

审稿时长

2.0 months

期刊介绍： Oncology Letters is a monthly, peer-reviewed journal, available in print and online, that focuses on all aspects of clinical oncology, as well as in vitro and in vivo experimental model systems relevant to the mechanisms of disease. The principal aim of Oncology Letters is to provide the prompt publication of original studies of high quality that pertain to clinical oncology, chemotherapy, oncogenes, carcinogenesis, metastasis, epidemiology and viral oncology in the form of original research, reviews and case reports.