Tara Randall, Inbar Shlomo, Emma Wells, Breanna Real, Ben Ma, Yarrow Linden, Jasmine Gamboa, Eran Friedler, Karl G. Linden
{"title":"评估紫外线 LED 消毒对废水排放过程中生物污损的控制作用","authors":"Tara Randall, Inbar Shlomo, Emma Wells, Breanna Real, Ben Ma, Yarrow Linden, Jasmine Gamboa, Eran Friedler, Karl G. Linden","doi":"10.2166/wrd.2024.131","DOIUrl":null,"url":null,"abstract":"<div><div data- reveal-group-><div><img alt=\"graphic\" data-src=\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\" path-from-xml=\"wreuse-d-23-00131gf01.tif\" src=\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\"/><div>View largeDownload slide</div></div></div><div content- data-reveal=\"data-reveal\"><div><img alt=\"graphic\" data-src=\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\" path-from-xml=\"wreuse-d-23-00131gf01.tif\" src=\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\"/><div>View largeDownload slide</div></div><i> </i><span>Close modal</span></div></div><p>A commercially available UVLED flow-through device, operating at 40 mJ/cm<sup>2</sup>, was examined for biofilm control on irrigation pipe material fed by wastewater effluent. Biofouling was monitored through total coliform counts, crystal violet (CV) staining, and ATP analyses. A UV fluence of 40 mJ/cm<sup>2</sup> at 280 nm retarded biofilm formation; however, complete biofilm prevention by UV treatment was not achieved despite a high inactivation of planktonic cells. After 5 days of the study, the total coliform and CV biofilm quantification assays between the UV-treated and control bioreactor coupons were not statistically different. The total coliform counts indicated a stable biofilm cell concentration was reached; the CV assay showed biofilm biomass accumulation with time. The ATP results revealed higher coupon ATP on the UV-treated coupons than the control coupons by day 5. The results provoke an interesting discussion surrounding the contribution of viable cells, represented by total coliforms, and extracellular polymeric substance (EPS) to total biofilm biomass. This study also highlighted a need for further investigation into the relationship between ATP responses and complex UV-stress responses of diverse microbial communities as opposed to pure bacteria cultures.</p>","PeriodicalId":17556,"journal":{"name":"Journal of Water Reuse and Desalination","volume":"245 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent\",\"authors\":\"Tara Randall, Inbar Shlomo, Emma Wells, Breanna Real, Ben Ma, Yarrow Linden, Jasmine Gamboa, Eran Friedler, Karl G. Linden\",\"doi\":\"10.2166/wrd.2024.131\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div data- reveal-group-><div><img alt=\\\"graphic\\\" data-src=\\\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\\\" path-from-xml=\\\"wreuse-d-23-00131gf01.tif\\\" src=\\\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\\\"/><div>View largeDownload slide</div></div></div><div content- data-reveal=\\\"data-reveal\\\"><div><img alt=\\\"graphic\\\" data-src=\\\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\\\" path-from-xml=\\\"wreuse-d-23-00131gf01.tif\\\" src=\\\"https://iwa.silverchair-cdn.com/iwa/content_public/journal/jwrd/14/1/10.2166_wrd.2024.131/2/m_wreuse-d-23-00131gf01.png?Expires=1714756187&Signature=fTVDlRLmYTtk39AywKFmLtVD63~8OkgcKdEoks5P9kpqjc1xal2n0tpZpwaqB0xQBxA~LH4Il1GyVSHTLU~onGINDQPMDHaNE7Dry169v6TR473FJyD-Lnhzb7u20LLm2tyuOrsUln2hmtW0Pk5yW-wOunkS4zJXv3dUlugxAf86gnMZusLG5w463d47UO0SA8YE4h7hMY-sUumxg~Uaov~H26AbUx~2cFv~wEgmTR6v5rTuostdiOF7UbeuDsYIJ52duNzIESLYAI1cvjkG3AtxAVkO1yfG7lQGQit0n7fiszEwyd31DxJPXwi4xrXbUMIOq8u36vqPGUE4eM2o4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA\\\"/><div>View largeDownload slide</div></div><i> </i><span>Close modal</span></div></div><p>A commercially available UVLED flow-through device, operating at 40 mJ/cm<sup>2</sup>, was examined for biofilm control on irrigation pipe material fed by wastewater effluent. Biofouling was monitored through total coliform counts, crystal violet (CV) staining, and ATP analyses. A UV fluence of 40 mJ/cm<sup>2</sup> at 280 nm retarded biofilm formation; however, complete biofilm prevention by UV treatment was not achieved despite a high inactivation of planktonic cells. After 5 days of the study, the total coliform and CV biofilm quantification assays between the UV-treated and control bioreactor coupons were not statistically different. The total coliform counts indicated a stable biofilm cell concentration was reached; the CV assay showed biofilm biomass accumulation with time. The ATP results revealed higher coupon ATP on the UV-treated coupons than the control coupons by day 5. The results provoke an interesting discussion surrounding the contribution of viable cells, represented by total coliforms, and extracellular polymeric substance (EPS) to total biofilm biomass. This study also highlighted a need for further investigation into the relationship between ATP responses and complex UV-stress responses of diverse microbial communities as opposed to pure bacteria cultures.</p>\",\"PeriodicalId\":17556,\"journal\":{\"name\":\"Journal of Water Reuse and Desalination\",\"volume\":\"245 1\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Water Reuse and Desalination\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2166/wrd.2024.131\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Water Reuse and Desalination","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2166/wrd.2024.131","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 0
摘要
View largeDownload slideView largeDownload slide Close modal一种市售的紫外发光二极管直流装置，以 40 mJ/cm2 的光照强度工作，用于控制由废水流入的灌溉管道材料上的生物膜。通过总大肠菌群计数、水晶紫（CV）染色和 ATP 分析来监测生物污垢。280 纳米波长下 40 mJ/cm2 的紫外线可延缓生物膜的形成；不过，尽管浮游细胞的灭活率很高，但紫外线处理并不能完全防止生物膜的形成。研究进行 5 天后，紫外线处理过的生物反应器和对照生物反应器的总大肠菌群和 CV 生物膜定量检测结果没有统计学差异。总大肠菌群计数表明生物膜细胞浓度已经稳定；CV 检测表明生物膜生物量随着时间的推移而积累。ATP 检测结果表明，到第 5 天时，紫外线处理过的生物膜比对照生物膜的 ATP 高。这些结果引发了关于以总大肠菌群为代表的可存活细胞和细胞外聚合物质（EPS）对生物膜总生物量的贡献的有趣讨论。这项研究还强调了进一步研究 ATP 反应与不同微生物群落（而不是纯细菌培养物）的复杂紫外线压力反应之间关系的必要性。
Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent
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A commercially available UVLED flow-through device, operating at 40 mJ/cm2, was examined for biofilm control on irrigation pipe material fed by wastewater effluent. Biofouling was monitored through total coliform counts, crystal violet (CV) staining, and ATP analyses. A UV fluence of 40 mJ/cm2 at 280 nm retarded biofilm formation; however, complete biofilm prevention by UV treatment was not achieved despite a high inactivation of planktonic cells. After 5 days of the study, the total coliform and CV biofilm quantification assays between the UV-treated and control bioreactor coupons were not statistically different. The total coliform counts indicated a stable biofilm cell concentration was reached; the CV assay showed biofilm biomass accumulation with time. The ATP results revealed higher coupon ATP on the UV-treated coupons than the control coupons by day 5. The results provoke an interesting discussion surrounding the contribution of viable cells, represented by total coliforms, and extracellular polymeric substance (EPS) to total biofilm biomass. This study also highlighted a need for further investigation into the relationship between ATP responses and complex UV-stress responses of diverse microbial communities as opposed to pure bacteria cultures.
期刊介绍：
Journal of Water Reuse and Desalination publishes refereed review articles, theoretical and experimental research papers, new findings and issues of unplanned and planned reuse. The journal welcomes contributions from developing and developed countries.
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