Evin Gunenc, Rumeysa Yesim Manap, Aleyna Cagan, Taylan Dogaroglu, Vatan Taskin, İrfan Kandemir, Ersin Dogac
{"title":"土耳其两种疟疾病媒 Anopheles superpictus 和 Anopheles sacharovi 种群基于微卫星的遗传结构","authors":"Evin Gunenc, Rumeysa Yesim Manap, Aleyna Cagan, Taylan Dogaroglu, Vatan Taskin, İrfan Kandemir, Ersin Dogac","doi":"10.1007/s11756-024-01667-9","DOIUrl":null,"url":null,"abstract":"<p><i>Anopheles</i> species are freshwater mosquitoes found in Turkey, where they have been the most important malaria vectors locally from the past to the present. The implementation of the integrated vector control methods have been difficult for these important malaria vectors as the different species have evolved different levels of resistance to the presently used insecticides. Population genetics research sheds light on gene flow across mosquito populations and, as a result, the likelihood of the spread of insecticide resistance genes across geographical regions. Considering the importance of these vectors, we analyzed the genetic structure and gene flow levels of six <i>An. sacharovi</i> and <i>An. superpictus</i> populations by using eight microsatellite markers. <i>Anopheles</i> samples were collected from different areas of the Aegean region of Turkey. Genotyping was carried out by using eight microsatellite markers on the ABI Prism 310 Genetic Analyzer. Genetic analyses indicated a relatively high level of genetic variation in <i>Anopheles</i> populations. The results revealed low genetic differentiation and moderate amounts of gene flow for both species among distinct populations. Also, significant genetic differentiation between the plurality of the population pairs analyzed was not found to be relevant to the geographic distances between populations. This is the first study to investigate the population genetics of the main malaria vectors, <i>An. sacharovi</i> and <i>An. superpictus</i>. Breeding habits, host feeding of <i>Anopheles</i> species, different collection times or points, climate, and variable effective population sizes may be some factors in the genetic diversity and genetic differentiation that have been observed between the populations.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"5 1","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microsatellite-based genetic structure of two malaria vectors Anopheles superpictus and Anopheles sacharovi populations from Turkey\",\"authors\":\"Evin Gunenc, Rumeysa Yesim Manap, Aleyna Cagan, Taylan Dogaroglu, Vatan Taskin, İrfan Kandemir, Ersin Dogac\",\"doi\":\"10.1007/s11756-024-01667-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Anopheles</i> species are freshwater mosquitoes found in Turkey, where they have been the most important malaria vectors locally from the past to the present. The implementation of the integrated vector control methods have been difficult for these important malaria vectors as the different species have evolved different levels of resistance to the presently used insecticides. Population genetics research sheds light on gene flow across mosquito populations and, as a result, the likelihood of the spread of insecticide resistance genes across geographical regions. Considering the importance of these vectors, we analyzed the genetic structure and gene flow levels of six <i>An. sacharovi</i> and <i>An. superpictus</i> populations by using eight microsatellite markers. <i>Anopheles</i> samples were collected from different areas of the Aegean region of Turkey. Genotyping was carried out by using eight microsatellite markers on the ABI Prism 310 Genetic Analyzer. Genetic analyses indicated a relatively high level of genetic variation in <i>Anopheles</i> populations. The results revealed low genetic differentiation and moderate amounts of gene flow for both species among distinct populations. Also, significant genetic differentiation between the plurality of the population pairs analyzed was not found to be relevant to the geographic distances between populations. This is the first study to investigate the population genetics of the main malaria vectors, <i>An. sacharovi</i> and <i>An. superpictus</i>. Breeding habits, host feeding of <i>Anopheles</i> species, different collection times or points, climate, and variable effective population sizes may be some factors in the genetic diversity and genetic differentiation that have been observed between the populations.</p>\",\"PeriodicalId\":8978,\"journal\":{\"name\":\"Biologia\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-04-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11756-024-01667-9\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11756-024-01667-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
疟蚊是在土耳其发现的淡水蚊子,从过去到现在一直是当地最重要的疟疾病媒。对这些重要的疟疾病媒来说,综合病媒控制方法的实施一直很困难,因为不同种类的蚊子对目前使用的杀虫剂产生了不同程度的抗药性。种群遗传学研究揭示了蚊子种群间基因流动的情况,因此也揭示了抗杀虫剂基因跨地理区域传播的可能性。考虑到这些媒介的重要性,我们使用 8 个微卫星标记分析了 6 个 An. sacharovi 和 An. superpictus 种群的遗传结构和基因流动水平。按蚊样本采集自土耳其爱琴海地区的不同区域。在 ABI Prism 310 基因分析仪上使用八个微卫星标记进行了基因分型。遗传分析表明,按蚊种群的遗传变异水平相对较高。结果表明,在不同的种群中,这两个物种的遗传分化程度较低,基因流动数量适中。此外,在所分析的多个种群对之间存在的明显遗传分化与种群之间的地理距离无关。这是首次对主要疟疾病媒沙加洛维氏疟蚊(An. sacharovi)和超级疟蚊(An. superpictus)的种群遗传学进行研究。繁殖习惯、按蚊的宿主取食、不同的采集时间或采集点、气候以及不同的有效种群规模可能是造成种群间遗传多样性和遗传分化的一些因素。
Microsatellite-based genetic structure of two malaria vectors Anopheles superpictus and Anopheles sacharovi populations from Turkey
Anopheles species are freshwater mosquitoes found in Turkey, where they have been the most important malaria vectors locally from the past to the present. The implementation of the integrated vector control methods have been difficult for these important malaria vectors as the different species have evolved different levels of resistance to the presently used insecticides. Population genetics research sheds light on gene flow across mosquito populations and, as a result, the likelihood of the spread of insecticide resistance genes across geographical regions. Considering the importance of these vectors, we analyzed the genetic structure and gene flow levels of six An. sacharovi and An. superpictus populations by using eight microsatellite markers. Anopheles samples were collected from different areas of the Aegean region of Turkey. Genotyping was carried out by using eight microsatellite markers on the ABI Prism 310 Genetic Analyzer. Genetic analyses indicated a relatively high level of genetic variation in Anopheles populations. The results revealed low genetic differentiation and moderate amounts of gene flow for both species among distinct populations. Also, significant genetic differentiation between the plurality of the population pairs analyzed was not found to be relevant to the geographic distances between populations. This is the first study to investigate the population genetics of the main malaria vectors, An. sacharovi and An. superpictus. Breeding habits, host feeding of Anopheles species, different collection times or points, climate, and variable effective population sizes may be some factors in the genetic diversity and genetic differentiation that have been observed between the populations.
期刊介绍:
Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.