聚糖性质对糖肽水凝胶结构和粘弹性能的影响

IF 1.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jonas Proksch, Marlene C. S. Dal Colle, Frederick Heinz, Robert F. Schmidt, Jacqueline Gottwald, Martina Delbianco, Bettina G. Keller, Michael Gradzielski, Ulrike Alexiev, Beate Koksch
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引用次数: 0

摘要

粘液是一种复杂的生物水凝胶,几乎对所有进出人体的物质都起着屏障作用。因此,粘液在生物医学和制药领域的应用日益受到关注。除了水之外,粘液中最丰富的成分是大量密集糖基化的粘蛋白,其糖蛋白高达 20 MDa,碳水化合物含量高达 80 wt%。在这里,我们设计并探索了一个糖基化肽库,以解构粘液的复杂性。我们使用特性良好的 hFF03 盘卷系统作为水凝胶形成肽支架,系统地探究了单个聚糖对二级结构的贡献以及所形成的水凝胶的形成和粘弹性特性。我们的研究表明,聚糖装饰不会影响α螺旋和线圈的形成,但会改变凝胶的硬度。通过使用振荡宏观流变学、动态光散射微观流变学和基于荧光寿命的纳米流变学,我们在多个长度尺度上对糖肽材料进行了表征。分子模拟显示,糖基化连接体可以延伸到溶剂中,但更多地与肽相互作用,从而可能改变自组装纤维的稳定性。这项系统性研究强调了糖基结构与水凝胶特性之间的相互作用,可为合成粘液模拟物的开发提供指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Impact of glycan nature on structure and viscoelastic properties of glycopeptide hydrogels

Impact of glycan nature on structure and viscoelastic properties of glycopeptide hydrogels

Impact of glycan nature on structure and viscoelastic properties of glycopeptide hydrogels

Mucus is a complex biological hydrogel that acts as a barrier for almost everything entering or exiting the body. It is therefore of emerging interest for biomedical and pharmaceutical applications. Besides water, the most abundant components are the large and densely glycosylated mucins, glycoproteins of up to 20 MDa and carbohydrate content of up to 80 wt%. Here, we designed and explored a library of glycosylated peptides to deconstruct the complexity of mucus. Using the well-characterized hFF03 coiled-coil system as a hydrogel-forming peptide scaffold, we systematically probed the contribution of single glycans to the secondary structure as well as the formation and viscoelastic properties of the resulting hydrogels. We show that glycan-decoration does not affect α-helix and coiled-coil formation while it alters gel stiffness. By using oscillatory macrorheology, dynamic light scattering microrheology, and fluorescence lifetime-based nanorheology, we characterized the glycopeptide materials over several length scales. Molecular simulations revealed that the glycosylated linker may extend into the solvent, but more frequently interacts with the peptide, thereby likely modifying the stability of the self-assembled fibers. This systematic study highlights the interplay between glycan structure and hydrogel properties and may guide the development of synthetic mucus mimetics.

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来源期刊
Journal of Peptide Science
Journal of Peptide Science 生物-分析化学
CiteScore
3.40
自引率
4.80%
发文量
83
审稿时长
1.7 months
期刊介绍: The official Journal of the European Peptide Society EPS The Journal of Peptide Science is a cooperative venture of John Wiley & Sons, Ltd and the European Peptide Society, undertaken for the advancement of international peptide science by the publication of original research results and reviews. The Journal of Peptide Science publishes three types of articles: Research Articles, Rapid Communications and Reviews. The scope of the Journal embraces the whole range of peptide chemistry and biology: the isolation, characterisation, synthesis properties (chemical, physical, conformational, pharmacological, endocrine and immunological) and applications of natural peptides; studies of their analogues, including peptidomimetics; peptide antibiotics and other peptide-derived complex natural products; peptide and peptide-related drug design and development; peptide materials and nanomaterials science; combinatorial peptide research; the chemical synthesis of proteins; and methodological advances in all these areas. The spectrum of interests is well illustrated by the published proceedings of the regular international Symposia of the European, American, Japanese, Australian, Chinese and Indian Peptide Societies.
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