Thi Trang Nguyen, The Dan Pham, Phat Tien Do, Kieu Thi Xuan Vo, Thi Van Anh Le, Tuan Anh Tran, Hoang Ha Chu, Jong-Seong Jeon, Huong Thi Mai To
{"title":"质粒蛋白 OsGER4 参与了由叶绿素介导的水稻冠根发育过程","authors":"Thi Trang Nguyen, The Dan Pham, Phat Tien Do, Kieu Thi Xuan Vo, Thi Van Anh Le, Tuan Anh Tran, Hoang Ha Chu, Jong-Seong Jeon, Huong Thi Mai To","doi":"10.1007/s12374-024-09424-w","DOIUrl":null,"url":null,"abstract":"<p>In rice (<i>Oryza sativa</i> L.), the root system plays different essential roles, from water and nutrient uptake to responding to environmental signals. The mechanisms underlying root development are complex and involve many phytohormones, of which auxin is the most important. This study investigates the involvement of OsGER4, a putative Germin-like protein, in auxin-mediated crown root development in rice. The expression study of <i>OsGER4</i> in the <i>crl1</i> mutant confirms that OsGER4 is connected to the CRL1 signaling pathway- a master regulator for crown root development. Transgenic rice carrying the <i>promGER4::GUS</i> reporter gene revealed that <i>OsGER4</i> is mainly expressed in the initiation and emergence zone of the crown and lateral root, such as epidermal cell, vasculature, and primordia under auxin treatment condition. Moreover, fewer crown roots of <i>osger4</i> knockout mutant lines than the wild type under auxin treatment suggests that OsGER4 might function as a regulator limiting auxin flux to root growth regions under stress conditions. Besides, protein localization experiments confirmed that OsGER4 localizes to plasmodesmata, which are intercellular channels that could facilitate auxin transport. Our findings suggest that OsGER4 might play a substantial role in regulating plasmodesmata conformation to regulate auxin flow resulting in crown root developmental in rice under stress conditions.</p>","PeriodicalId":16762,"journal":{"name":"Journal of Plant Biology","volume":"31 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Plasmodesmal Protein OsGER4 is Involved in Auxin Mediated Crown Root Development in Rice\",\"authors\":\"Thi Trang Nguyen, The Dan Pham, Phat Tien Do, Kieu Thi Xuan Vo, Thi Van Anh Le, Tuan Anh Tran, Hoang Ha Chu, Jong-Seong Jeon, Huong Thi Mai To\",\"doi\":\"10.1007/s12374-024-09424-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In rice (<i>Oryza sativa</i> L.), the root system plays different essential roles, from water and nutrient uptake to responding to environmental signals. The mechanisms underlying root development are complex and involve many phytohormones, of which auxin is the most important. This study investigates the involvement of OsGER4, a putative Germin-like protein, in auxin-mediated crown root development in rice. The expression study of <i>OsGER4</i> in the <i>crl1</i> mutant confirms that OsGER4 is connected to the CRL1 signaling pathway- a master regulator for crown root development. Transgenic rice carrying the <i>promGER4::GUS</i> reporter gene revealed that <i>OsGER4</i> is mainly expressed in the initiation and emergence zone of the crown and lateral root, such as epidermal cell, vasculature, and primordia under auxin treatment condition. Moreover, fewer crown roots of <i>osger4</i> knockout mutant lines than the wild type under auxin treatment suggests that OsGER4 might function as a regulator limiting auxin flux to root growth regions under stress conditions. Besides, protein localization experiments confirmed that OsGER4 localizes to plasmodesmata, which are intercellular channels that could facilitate auxin transport. Our findings suggest that OsGER4 might play a substantial role in regulating plasmodesmata conformation to regulate auxin flow resulting in crown root developmental in rice under stress conditions.</p>\",\"PeriodicalId\":16762,\"journal\":{\"name\":\"Journal of Plant Biology\",\"volume\":\"31 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12374-024-09424-w\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12374-024-09424-w","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
The Plasmodesmal Protein OsGER4 is Involved in Auxin Mediated Crown Root Development in Rice
In rice (Oryza sativa L.), the root system plays different essential roles, from water and nutrient uptake to responding to environmental signals. The mechanisms underlying root development are complex and involve many phytohormones, of which auxin is the most important. This study investigates the involvement of OsGER4, a putative Germin-like protein, in auxin-mediated crown root development in rice. The expression study of OsGER4 in the crl1 mutant confirms that OsGER4 is connected to the CRL1 signaling pathway- a master regulator for crown root development. Transgenic rice carrying the promGER4::GUS reporter gene revealed that OsGER4 is mainly expressed in the initiation and emergence zone of the crown and lateral root, such as epidermal cell, vasculature, and primordia under auxin treatment condition. Moreover, fewer crown roots of osger4 knockout mutant lines than the wild type under auxin treatment suggests that OsGER4 might function as a regulator limiting auxin flux to root growth regions under stress conditions. Besides, protein localization experiments confirmed that OsGER4 localizes to plasmodesmata, which are intercellular channels that could facilitate auxin transport. Our findings suggest that OsGER4 might play a substantial role in regulating plasmodesmata conformation to regulate auxin flow resulting in crown root developmental in rice under stress conditions.
期刊介绍:
Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants.
The Journal publishes the following categories of paper:
Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged.
Reviews -- Invited by the EiC.
Brief Communications -- Concise but independent report representing significant contribution to plant science.
The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.