Alexis A.M. Julian , John Derek Scasta , Whitney C. Stewart
{"title":"利用粪便 DNA 代谢编码和草料采样为绵羊冬季日粮参数化提供矿物质营养管理信息","authors":"Alexis A.M. Julian , John Derek Scasta , Whitney C. Stewart","doi":"10.1016/j.rama.2024.03.003","DOIUrl":null,"url":null,"abstract":"<div><p>Mineral nutrition deficiencies commonly occur in extensive sheep production systems, yet often coincide with critical production periods of breeding and gestation when quality and quantity of winter range are most limited. Ewe productivity may be limited by clinical and subclinical mineral deficiencies when grazing extensive rangelands during the winter months. The objectives of this study were to utilize fecal meta-barcoding DNA (f.DNA) to estimate dietary composition of ewes by plant morphological group (monocots and dicots), and quantified plant nutritional composition from clipped biomass to estimate adequacy of macro- and micro-minerals of the basal diet across extensively grazed sheep operations. Ewe fecal samples for f.DNA were collected from 19 extensive sheep operations across Wyoming and Colorado, USA, and analyzed at the plant morphological group level to estimate dietary composition as 1) Monocot (≥ 70%), 2) Dicot (≥ 70%), or 3) Mixed (monocot and dicot co-dominated, both proportions < 70%). Pooled forage species nutritional composition within morphological group (monocot vs. dicot) was then multiplied by the proportion of monocotyledonous species or dicotyledonous species and intake estimates of 2% of BW on an 80 kg ewe to estimate macro- and micro-mineral intake. Results from f.DNA indicated 36% of operations were categorized as Monocot (≥ 70% dietary component), 42% Dicot (≥ 70% dietary component), or 21% Mixed. A significant effect of plant morphological group category was observed for CP, Ca, K, Mg, and S (<em>P</em> < 0.05) where dicot dominated rangelands contained higher concentrations of these minerals compared to monocot or mixed monocot-dicot rangelands. Overall, dicot dominated rangelands provided greater macro- and micro-mineral content ultimately meeting more requirements for breeding and gestation than monocot or mixed monocot-dicot rangelands. Therefore, targeted supplementation must be considered for ewes on monocot or mixed monocot-dicot rangelands during winter months that coincide with critical production stages.</p></div>","PeriodicalId":49634,"journal":{"name":"Rangeland Ecology & Management","volume":"94 ","pages":"Pages 168-177"},"PeriodicalIF":2.4000,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1550742424000435/pdfft?md5=b59903228c0d2a0d135665a3126fc2f3&pid=1-s2.0-S1550742424000435-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Sheep Winter Diets Parameterized With Fecal DNA Metabarcoding and Forage Sampling Informs Mineral Nutrition Management\",\"authors\":\"Alexis A.M. Julian , John Derek Scasta , Whitney C. Stewart\",\"doi\":\"10.1016/j.rama.2024.03.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Mineral nutrition deficiencies commonly occur in extensive sheep production systems, yet often coincide with critical production periods of breeding and gestation when quality and quantity of winter range are most limited. Ewe productivity may be limited by clinical and subclinical mineral deficiencies when grazing extensive rangelands during the winter months. The objectives of this study were to utilize fecal meta-barcoding DNA (f.DNA) to estimate dietary composition of ewes by plant morphological group (monocots and dicots), and quantified plant nutritional composition from clipped biomass to estimate adequacy of macro- and micro-minerals of the basal diet across extensively grazed sheep operations. Ewe fecal samples for f.DNA were collected from 19 extensive sheep operations across Wyoming and Colorado, USA, and analyzed at the plant morphological group level to estimate dietary composition as 1) Monocot (≥ 70%), 2) Dicot (≥ 70%), or 3) Mixed (monocot and dicot co-dominated, both proportions < 70%). Pooled forage species nutritional composition within morphological group (monocot vs. dicot) was then multiplied by the proportion of monocotyledonous species or dicotyledonous species and intake estimates of 2% of BW on an 80 kg ewe to estimate macro- and micro-mineral intake. Results from f.DNA indicated 36% of operations were categorized as Monocot (≥ 70% dietary component), 42% Dicot (≥ 70% dietary component), or 21% Mixed. A significant effect of plant morphological group category was observed for CP, Ca, K, Mg, and S (<em>P</em> < 0.05) where dicot dominated rangelands contained higher concentrations of these minerals compared to monocot or mixed monocot-dicot rangelands. Overall, dicot dominated rangelands provided greater macro- and micro-mineral content ultimately meeting more requirements for breeding and gestation than monocot or mixed monocot-dicot rangelands. 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Sheep Winter Diets Parameterized With Fecal DNA Metabarcoding and Forage Sampling Informs Mineral Nutrition Management
Mineral nutrition deficiencies commonly occur in extensive sheep production systems, yet often coincide with critical production periods of breeding and gestation when quality and quantity of winter range are most limited. Ewe productivity may be limited by clinical and subclinical mineral deficiencies when grazing extensive rangelands during the winter months. The objectives of this study were to utilize fecal meta-barcoding DNA (f.DNA) to estimate dietary composition of ewes by plant morphological group (monocots and dicots), and quantified plant nutritional composition from clipped biomass to estimate adequacy of macro- and micro-minerals of the basal diet across extensively grazed sheep operations. Ewe fecal samples for f.DNA were collected from 19 extensive sheep operations across Wyoming and Colorado, USA, and analyzed at the plant morphological group level to estimate dietary composition as 1) Monocot (≥ 70%), 2) Dicot (≥ 70%), or 3) Mixed (monocot and dicot co-dominated, both proportions < 70%). Pooled forage species nutritional composition within morphological group (monocot vs. dicot) was then multiplied by the proportion of monocotyledonous species or dicotyledonous species and intake estimates of 2% of BW on an 80 kg ewe to estimate macro- and micro-mineral intake. Results from f.DNA indicated 36% of operations were categorized as Monocot (≥ 70% dietary component), 42% Dicot (≥ 70% dietary component), or 21% Mixed. A significant effect of plant morphological group category was observed for CP, Ca, K, Mg, and S (P < 0.05) where dicot dominated rangelands contained higher concentrations of these minerals compared to monocot or mixed monocot-dicot rangelands. Overall, dicot dominated rangelands provided greater macro- and micro-mineral content ultimately meeting more requirements for breeding and gestation than monocot or mixed monocot-dicot rangelands. Therefore, targeted supplementation must be considered for ewes on monocot or mixed monocot-dicot rangelands during winter months that coincide with critical production stages.
期刊介绍:
Rangeland Ecology & Management publishes all topics-including ecology, management, socioeconomic and policy-pertaining to global rangelands. The journal''s mission is to inform academics, ecosystem managers and policy makers of science-based information to promote sound rangeland stewardship. Author submissions are published in five manuscript categories: original research papers, high-profile forum topics, concept syntheses, as well as research and technical notes.
Rangelands represent approximately 50% of the Earth''s land area and provision multiple ecosystem services for large human populations. This expansive and diverse land area functions as coupled human-ecological systems. Knowledge of both social and biophysical system components and their interactions represent the foundation for informed rangeland stewardship. Rangeland Ecology & Management uniquely integrates information from multiple system components to address current and pending challenges confronting global rangelands.