利用粪便 DNA 代谢编码和草料采样为绵羊冬季日粮参数化提供矿物质营养管理信息

IF 2.4 3区 环境科学与生态学 Q2 ECOLOGY
Alexis A.M. Julian , John Derek Scasta , Whitney C. Stewart
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引用次数: 0

摘要

矿物质营养缺乏症通常发生在广阔的绵羊生产系统中,但往往与繁殖和妊娠的关键生产期重合,而此时冬季牧场的质量和数量最为有限。在冬季放牧广阔牧场时,临床和亚临床矿物质缺乏可能会限制母羊的生产率。本研究的目的是利用排泄物元条形码 DNA(f.DNA)按植物形态组(单子叶植物和双子叶植物)估算母羊的日粮组成,并从剪下的生物量中量化植物营养成分,以估算大面积放牧的羊群基础日粮中是否含有充足的宏量和微量矿物质。从美国怀俄明州和科罗拉多州的 19 个大面积牧羊场收集母羊粪便样本进行 f.DNA 分析,并在植物形态组水平上进行分析,以估算日粮组成:1)单子叶植物(≥ 70%);2)双子叶植物(≥ 70%);或 3)混合植物(单子叶植物和双子叶植物共占优势,两者比例均为 70%)。然后,将形态组(单子叶植物与双子叶植物)内汇总的饲草物种营养成分乘以单子叶植物或双子叶植物物种的比例,再乘以 80 千克母羊体重 2% 的摄入量估计值,以估算宏观和微观矿物质的摄入量。f.DNA 的结果表明,36% 的操作被归类为单子叶植物(≥ 70% 的日粮成分)、42% 的双子叶植物(≥ 70% 的日粮成分)或 21% 的混合植物。在 CP、Ca、K、Mg 和 S 方面,观察到植物形态组类别的显着影响(P < 0.05),与单子叶植物或单子叶植物-双子叶植物混合牧场相比,双子叶植物为主的牧场含有更高浓度的这些矿物质。总体而言,与单子叶植物或单子叶-双子叶植物混合牧场相比,双子叶植物为主的牧场提供了更多的宏量和微量矿物质,最终满足了繁殖和妊娠的更多需求。因此,在冬季关键生产阶段,单子叶或单子叶-双子叶混合牧场上的母羊必须考虑有针对性地补充矿物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sheep Winter Diets Parameterized With Fecal DNA Metabarcoding and Forage Sampling Informs Mineral Nutrition Management

Mineral nutrition deficiencies commonly occur in extensive sheep production systems, yet often coincide with critical production periods of breeding and gestation when quality and quantity of winter range are most limited. Ewe productivity may be limited by clinical and subclinical mineral deficiencies when grazing extensive rangelands during the winter months. The objectives of this study were to utilize fecal meta-barcoding DNA (f.DNA) to estimate dietary composition of ewes by plant morphological group (monocots and dicots), and quantified plant nutritional composition from clipped biomass to estimate adequacy of macro- and micro-minerals of the basal diet across extensively grazed sheep operations. Ewe fecal samples for f.DNA were collected from 19 extensive sheep operations across Wyoming and Colorado, USA, and analyzed at the plant morphological group level to estimate dietary composition as 1) Monocot (≥ 70%), 2) Dicot (≥ 70%), or 3) Mixed (monocot and dicot co-dominated, both proportions < 70%). Pooled forage species nutritional composition within morphological group (monocot vs. dicot) was then multiplied by the proportion of monocotyledonous species or dicotyledonous species and intake estimates of 2% of BW on an 80 kg ewe to estimate macro- and micro-mineral intake. Results from f.DNA indicated 36% of operations were categorized as Monocot (≥ 70% dietary component), 42% Dicot (≥ 70% dietary component), or 21% Mixed. A significant effect of plant morphological group category was observed for CP, Ca, K, Mg, and S (P < 0.05) where dicot dominated rangelands contained higher concentrations of these minerals compared to monocot or mixed monocot-dicot rangelands. Overall, dicot dominated rangelands provided greater macro- and micro-mineral content ultimately meeting more requirements for breeding and gestation than monocot or mixed monocot-dicot rangelands. Therefore, targeted supplementation must be considered for ewes on monocot or mixed monocot-dicot rangelands during winter months that coincide with critical production stages.

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来源期刊
Rangeland Ecology & Management
Rangeland Ecology & Management 农林科学-环境科学
CiteScore
4.60
自引率
13.00%
发文量
87
审稿时长
12-24 weeks
期刊介绍: Rangeland Ecology & Management publishes all topics-including ecology, management, socioeconomic and policy-pertaining to global rangelands. The journal''s mission is to inform academics, ecosystem managers and policy makers of science-based information to promote sound rangeland stewardship. Author submissions are published in five manuscript categories: original research papers, high-profile forum topics, concept syntheses, as well as research and technical notes. Rangelands represent approximately 50% of the Earth''s land area and provision multiple ecosystem services for large human populations. This expansive and diverse land area functions as coupled human-ecological systems. Knowledge of both social and biophysical system components and their interactions represent the foundation for informed rangeland stewardship. Rangeland Ecology & Management uniquely integrates information from multiple system components to address current and pending challenges confronting global rangelands.
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